研究成果

• 原著論文
• 総説・解説等

原著論文

2022

• Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant
  Y. Doi, J. Watanabe, R. Nii, T. Tsukamoto, M. Demura, Y. Sudo, T. Kikukawa
  Sci Rep., 12, 16422 (2022)


• Reisomerization of retinal represents a molecular switch mediating Na⁺ uptake and release by a bacterial sodium-pumping rhodopsin
  T. Fujisawa, K. Kinoue, R. Seike, T. Kikukawa, M. Unno
  J. Biol. Chem., 296, 102366 (2022)

2021

• Pore Size Distributions Related with Spontaneous Purple Membrane Stacking in Porous Hydrogels
  M. Karasawa, Y. Yokoyama, K. Takiguchi, H. Takahashi, T. Kikukawa, M. Sonoyama, K. Takenaka
  J. Phys. Soc. Jpn., 90, 103801 (2021)


• Replaceability of Schiff base proton donors in light-driven proton pump rhodopsins
  S. Sasaki, J. Tamogami, K. Nishiya, M. Demura, T. Kikukawa
  J. Biol. Chem., 97, 101013 (2021)


• Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization
  M. Kikuchi, K. Kojima, S. Nakao, S. Yoshizawa, S. Kawanishi, A. Shibukawa, T. Kikukawa, Y. Sudo
  Sci Rep. , 11, 14765 (2021)


• Comparison of Functionality and Structural Stability of Bacteriorhodopsin Reconstituted in Partially Fluorinated Dimyristoylphosphatidylcholine Liposomes with Different Perfluoroalkyl Chain Lengths
  M. Hashimoto, Y. Murai, K. Morita, T. Kikukawa, T. Takagi, H. Takahashi, Y. Yokoyama, H. Amii, M. Sonoyama
  Biochim. Biophys. Acta, Biomembr., 1863, 183686 (2021)


• Protonation State of a Histidine Residue in Human Oligopeptide Transporter 1 (hPEPT1) Regulates hPEPT1-Mediated Efflux Activity
  A. Omori, Y. Fujisawa, S. Sasaki, K. Shimono, T. Kikukawa, S. Miyauchi
  Biol. Pharm. Bull., 44, 678-685 (2021)


• Real-Time Identification of Two Substrate-Binding Intermediates for the Light-Driven Sodium Pump Rhodopsin
  T. Kato, T. Tsukamoto, M. Demura, T. Kikukawa
  J. Biol. Chem., 296, 100792 (2021)


• Preference of Proteomonas sulcata anion channelrhodopsin for NO₃⁻ revealed using a pH electrode method
  C. Kikuchi, H. Kurane, T. Watanabe, M. Demura, T. Kikukawa, T. Tsukamoto
  Sci Rep. , 11, 7908 (2021)


• Effects of salt and gel network structures on purple membrane stacking in hydrogels immobilized with poly(vinyl alcohol)
  Y. Yokoyama, S. Yano, R. Kurita, M. Karasawa, H. Tanaka, H. Takahashi, T. Kikukawa, M. Sonoyama, K. Takenaka
  J. Appl. Phys., 129, 014701 (2021)


• Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport
  Y. Nakamizo, T. Fujisawa, T. Kikukawa, A. Okamura, H. Baba, M. Unno
  Phys. Chem. Chem. Phys., 23, 2072-2079 (2021)

2020

• Lokiarchaeota archaeon schizorhodopsin-2 (LaSzR2) is an inward proton pump displaying a characteristic feature of acid-induced spectral blue-shift
  K. Kojima, S. Yoshizawa, M. Hasegawa, M. Nakama, M. Kurihara, T. Kikukawa, Y. Sudo
  Sci Rep. , 10, 20857 (2020)


• “Watching” a Molecular Twist in a Protein by Raman Optical Activity
  J. Matsu, T. Kikukawa, T. Fujisawa, W. D. Hoff, M. Unno
  J. Phys. Chem. Lett., 11, 8579–8584


• Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin.
  K. Murabe, T. Tsukamoto, T. Aizawa, M. Demura, T. Kikukawa
  J Am Chem Soc., 142, 16023-16030 (2020)


• Spectroscopic Characterization of Halorhodopsin Reconstituted into Nanodisks Using Native Lipids.
  A. Yamamoto, T. Tsukamoto, K. Suzuki, E. Hashimoto, Y. Kobashigawa, K. Shibasaki, T. Uchida, F. Inagaki, M. Demura, K. Ishimori
  Biophys J., 118, 2853-2865 (2020)


• Methodology for Further Thermostabilization of an Intrinsically Thermostable Membrane Protein Using Amino Acid Mutations with Its Original Function Being Retained
  S. Yasuda, T. Akiyama, S. Nemoto, T. Hayashi, T. Ueta, K. Kojima, T. Tsukamoto, S. Nagatoishi, K. Tsumoto, Y. Sudo, M. Kinoshita, T. Murata
  J. Chem. Inf. and Model., 60, 1709-1716 (2020)

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解説・総説など

• 菊川峰志, Unique Cl^- pump rhodopsin with close similarity to H⁺ pump rhodopsin, Biophys. Physicobiol., Vol.18, 317-326 (2021)
• 菊川峰志, Cl^–ポンプロドプシンの分子機構とH⁺ポンプへの機能変換, 生物物理 61: 12-15 (2021)
• Kikukawa, T., Functional Mechanism of Cl⁻-Pump Rhodopsin and Its Conversion into H⁺ Pump, In Optogenetics 2nd Ed.: Light-Sensing Proteins and Their Applications in Neuroscience and Beyond (Yawo., H., Kandori, H., Koizumi, A., Kageyama, R., Eds.), pp 55-71, ISBN: 978-981-15-8763-4, Springer (2021)
• 出村誠、シルクと持続可能な開発目標(SDGs)、繊維機械学会誌『せんい』, Vol.73 (2020)
• 出村誠・朝倉哲郎、タンパク質、『基礎高分子科学』改訂版・９章 生体高分子、東京化学同人、2020
• 出村誠、カイコ絹糸の模倣からイノベーションへ, 特集 生物機能模倣技術, アグリバイオ, vol.1, 235-239 (2017)
• 菊川峰志, プロテオロドプシン, 「光と生命の事典」, pp. 58-59, ISBN: 978-4-254-17161-7, 朝倉書店 (2016)
• 出村誠、光のエネルギー利用27 ハロロドプシン『光と生命の事典』（日本光生物学協会「光と生命の事典」編集委員会 編）朝倉書店 2016.2.25
• Takashi Kikukawa, Naoki Kamo and Makoto Demura, Photochemistry of Halorhodopsin in Optogenetics (Eds. Hideki Kandori, Hiromu Yawo, Amane Koizumi) Springer 2015, pp47-62.

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