原著論文・Recent Publication

2022

• Mutations conferring SO42− pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant
  Y. Doi, J. Watanabe, R. Nii, T. Tsukamoto, M. Demura, Y. Sudo, T. Kikukawa
  Sci Rep., 12, 16422 (2022)


• Reisomerization of retinal represents a molecular switch mediating Na⁺ uptake and release by a bacterial sodium-pumping rhodopsin
  T. Fujisawa, K. Kinoue, R. Seike, T. Kikukawa, M. Unno
  J. Biol. Chem., 296, 102366 (2022)

2021

• Pore Size Distributions Related with Spontaneous Purple Membrane Stacking in Porous Hydrogels
  M. Karasawa, Y. Yokoyama, K. Takiguchi, H. Takahashi, T. Kikukawa, M. Sonoyama, K. Takenaka
  J. Phys. Soc. Jpn., 90, 103801 (2021)


• Replaceability of Schiff base proton donors in light-driven proton pump rhodopsins
  S. Sasaki, J. Tamogami, K. Nishiya, M. Demura, T. Kikukawa
  J. Biol. Chem., 97, 101013 (2021)


• Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization
  M. Kikuchi, K. Kojima, S. Nakao, S. Yoshizawa, S. Kawanishi, A. Shibukawa, T. Kikukawa, Y. Sudo
  Sci Rep. , 11, 14765 (2021)


• Comparison of Functionality and Structural Stability of Bacteriorhodopsin Reconstituted in Partially Fluorinated Dimyristoylphosphatidylcholine Liposomes with Different Perfluoroalkyl Chain Lengths
  M. Hashimoto, Y. Murai, K. Morita, T. Kikukawa, T. Takagi, H. Takahashi, Y. Yokoyama, H. Amii, M. Sonoyama
  Biochim. Biophys. Acta, Biomembr., 1863, 183686 (2021)


• Preference of Proteomonas sulcata anion channelrhodopsin for NO₃⁻ revealed using a pH electrode method
  C. Kikuchi, H. Kurane, T. Watanabe, M. Demura, T. Kikukawa, T. Tsukamoto
  Sci Rep. , 11, 7908 (2021)


• Effects of salt and gel network structures on purple membrane stacking in hydrogels immobilized with poly(vinyl alcohol)
  Y. Yokoyama, S. Yano, R. Kurita, M. Karasawa, H. Tanaka, H. Takahashi, T. Kikukawa, M. Sonoyama, K. Takenaka
  J. Appl. Phys., 129, 014701 (2021)


• Low-temperature Raman spectroscopy of sodium-pump rhodopsin from Indibacter alkaliphilus: Insight of Na+ binding for active Na+ transport
  Y. Nakamizo, T. Fujisawa, T. Kikukawa, A. Okamura, H. Baba, M. Unno
  Phys. Chem. Chem. Phys., 23, 2072-2079 (2021)

2020

• Lokiarchaeota archaeon schizorhodopsin-2 (LaSzR2) is an inward proton pump displaying a characteristic feature of acid-induced spectral blue-shift
  K. Kojima, S. Yoshizawa, M. Hasegawa, M. Nakama, M. Kurihara, T. Kikukawa, Y. Sudo
  Sci Rep. , 10, 20857 (2020)


• “Watching” a Molecular Twist in a Protein by Raman Optical Activity
  J. Matsu, T. Kikukawa, T. Fujisawa, W. D. Hoff, M. Unno
  J. Phys. Chem. Lett., 11, 8579–8584


• Direct Detection of the Substrate Uptake and Release Reactions of the Light-Driven Sodium-Pump Rhodopsin.
  K. Murabe, T. Tsukamoto, T. Aizawa, M. Demura, T. Kikukawa
  J Am Chem Soc., 142, 16023-16030 (2020)


• Spectroscopic Characterization of Halorhodopsin Reconstituted into Nanodisks Using Native Lipids.
  A. Yamamoto, T. Tsukamoto, K. Suzuki, E. Hashimoto, Y. Kobashigawa, K. Shibasaki, T. Uchida, F. Inagaki, M. Demura, K. Ishimori
  Biophys J., 118, 2853-2865 (2020)


• Methodology for Further Thermostabilization of an Intrinsically Thermostable Membrane Protein Using Amino Acid Mutations with Its Original Function Being Retained
  S. Yasuda, T. Akiyama, S. Nemoto, T. Hayashi, T. Ueta, K. Kojima, T. Tsukamoto, S. Nagatoishi, K. Tsumoto, Y. Sudo, M. Kinoshita, T. Murata
  J. Chem. Inf. and Model., 60, 1709-1716 (2020)

2019

• Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome
  T. Sato, T. Kikukawa, R. Miyoshi, K. Kajimoto, C. Yonekawa, T. Fujisawa, M. Unno, T. Eki, Y. Hirose
  J. Biol. Chem., 294, 1767 (2019)


• Low-Temperature Raman Spectroscopy of Halorhodopsin from Natronomonas pharaonis: Structural Discrimination of Blue-Shifted and Red-Shifted Photoproducts
  T. Fujisawa, H. Kiyota, T. Kikukawa, M. Unno
  Biochemistry, 58, 4159-4167 (2019)


• Functional importance of the oligomer formation of the cyanobacterial H⁺ pump Gloeobacter rhodopsin
  A. Iizuka, K. Kajimoto, T. Fujisawa, T. Tsukamoto, T. Aizawa, N. Kamo, K.H. Jung, M. Unno, M. Demura, T. Kikukawa
  Scientific Reports, 9, 10711 (2019)


• Two Consecutive Polar Amino Acids at the End of Helix E are Important for Fast Turnover of the Archaerhodopsin Photocycle
  X. Geng, G. Dai, L. Chao, D. Wen, T. Kikukawa, T. Iwasa
  Photochemistry and Photobiology, 95, 980-989 (2019)


• Quantitation of the neural silencing activity of anion channelrhodopsins in Caenorhabditis elegans and their applicability for long-term illumination
  T. Yamanashi, M. Maki, K. Kojima, A. Shibukawa, T. Tsukamoto, S. Chowdhury, A. Yamanaka, S. Takagi, Y. Sudo
  Sci. Rep., 9, 7863 (2019)


• Comparison of two different partially fluorinated phosphatidylcholines with the perfluorobutyl group on thermotropic properties of the bilayer membrane and reconstituted bacteriorhodopsin.
  T. Yanagi, T. Takagi, H. Takahashi, T. Kikukawa, H. Amii, M. Sonoyama
  Biophys. Rev., 11, 395-398 (2019)


• Studies on the quality control of fermented brown rice and rice bran with Aspergillus oryzae by metabolome analysis using ¹H-NMR
  Y. Horie, H. Nemoto, J. Fujita, S. Ikegawa, Y. Kumaki, Y. Ohnishi, H. , M. Demura, T. Aizawa
  Nippon Shokuhin Kagaku Kogaku Kaishi, 66, 139-146 (2019)


• Stability of the two-dimensional lattice of bacteriorhodopsin reconstituted in partially fluorinated phosphatidylcholine bilayers
  H. Takahashi, M. Yoshino, K. Morita, T. Takagi, Y. Yokoyama, T. Kikukawa, H. Amii, T. Kanamori, M. Sonoyama
  Biochimica et Biophysica Acta - Biomembranes, 1861, 631-642 (2019)


• Photochemical study of a cyanobacterial chloride-ion pumping rhodopsin
  T. Hasemi, T. Kikukawaa, Y. Watanabe, T. Aizawa, S. Miyauchi, N. Kamo, M. Demura
  Biochim. Biophys. Acta - Bioenergetics, 1860, 136-146 (2019)

2018

• Correction to “High Thermal Stability of Oligomeric Assemblies of Thermophilic Rhodopsin in a Lipid Environment”
  T. Shionoya, M. Mizuno, T. Tsukamoto, K. Ikeda, H. Seki, K. Kojima, M. Shibata, I. Kawamura, Y. Sudo, Y. Mizutani
  J. Phys. Chem. B, 122, 9826 (2018)


• Low-temperature Raman spectroscopy reveals small chromophore distortion in primary photointermediate of proteorhodopsin
  T. Fujisawa, M. Abe, J. Tamogami, T. Kikukawa, N. Kamo, M. Unno
  FEBS Letters, 592, 3054-3061 (2018)


• Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl− concentration
  T. Tsukamoto, C. Kikuchi, H. Suzuki, T. Aizawa,T. Kikukawa, M. Demura
  Scientific Reports, 8, 13445 (2018)


• Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative
  R. Takayama, A. Kaneko, T. Okitsu, S. P. Tsunoda, K. Shimono, M. Mizuno, K. Kojima, T. Tsukamoto, H. Kandori, Y. Mizutani, A. Wada, Y. Sudo
  J. Phys. Chem. Lett., 9, 2857-2862 (2018)


• Photocycle of Sensory Rhodopsin II from Halobacterium salinarum (HsSRII): Mutation of D103 Accelerates M Decay and Changes the Decay Pathway of a 13‐cis O‐like Species
  Gang Dai, Xiong Geng, Chaoluomeng, J. Tamogami, T. Kikukawa, M. Demura, N. Kamo, T. Iwasa
  Photochemistry and Photobiology, 94, 705–714 (2018)


• High Thermal Stability of Oligomeric Assemblies of Thermophilic Rhodopsin in a Lipid Environment
  T. Shionoya, M. Mizuno, T. Tsukamoto, K. Ikeda, H. Seki, K. Kojima, M. Shibata, I. Kawamura, Y. Sudo, Y. Mizutani
  J. Phys. Chem. B, 122, 6945-6953 (2018)


• Interhelical interactions between D92 and C218 in the cytoplasmic domain regulate proton uptake upon N-decay in the proton transport of Acetabularia rhodopsin II
  J. Tamogami, T. Kikukawa, K. Ohkawa, N. Ohsawa, T. Nara, M. Demura, S. Miyauchi, T. Kimura-Someya, M. Shirouzu, S. Yokoyama, K. Shimono, N. Kamo
  Journal of Photochemistry & Photobiology, B: Biology, 183, 35-45 (2018)


• Presence of a Haloarchaeal Halorhodopsin-Like Cl- Pump in Marine Bacteria
  Y. Nakajima, T. Tsukamoto, Y. Kumagai, Y. Ogura, T. Hayashi, J. Song, T. Kikukawa, M. Demura, K. Kogure, Y. Sudo, S. Yoshizawa
  Microbes Environ., 33, 89-97 (2018)


• Spectroscopic Characteristics of Rubricoccus marinus Xenorhodopsin (RmXeR) and a Putative Model for its Inward H+ Transport mechanism
  S. Inoue, S. Yoshizawa, Y. Nakajima, K. Kojima, T. Tsukamoto, T. Kikukawa, Y. Sudo
  Phys. Chem. Chem. Phys., 20, 3172-3183 (2018)

2017

• Targeted Activation of Molecular Transportation by Visible Light
  A. S. Amrutha, K. R. Sunil Kumar, T. Kikukawa, N. Tamaoki
  ACS Nano, 11, 12292–12301 (2017)


• Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system
  Md. R. Kuddus, M. Yamano, F. Rumi, T. Kikukawa, M. Demura, T. Aizawa
  Biotechnology progress, 33, 1520-1528 (2017)


• Spontaneous stacking of purple membranes during immobilization with physical cross-linked poly(vinyl alcohol) hydrogel with retaining native-like functionality of bacteriorhodopsin
  Y. Yokoyama, H. Tanaka, S. Yano, H. Takahashi, T. Kikukawa, M. Sonoyama, K. Takenaka
  J. Appl. Phys., 121, 204701 (2017)


• Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus
  K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno, Masashi
  Journal of Physical Chemistry B, 121, 4431-4437 (2017)


• Existence of two O-like intermediates in the photocycle of Acetabularia rhodopsin II, a light-driven proton pump from a marine alga
  J. Tamogami, T. Kikukawa, T. Nara, M. Demura, T. Kimura-Someya, M. Shirouzu, S. Yokoyama, S. Miyauchi, K. Shimono, N. Kamo
  Biophysics and Physicobiology, 14, 49-55 (2017)


• Implications for the Light-Driven Chloride Ion Transport Mechanism of Nonlabens marinus Rhodopsin 3 by Its Photochemical Characteristics
  T. Tsukamoto, S. Yoshizawa, T. Kikukawa, M. Demura, Y. Sudo
  Journal of Physical Chemistry B, 121, 2017-2038 (2017)

2016

• Photochemical characterization of actinorhodopsin and its functional existence in the natural host
  S. Nakamura, T. Kikukawa, J. Tamogami, M. Kamiya, T. Aizawa, Martin W Hahn, K. Ihara, N. Kamo, M. Demura
  Biochim. Biophys. Acta - Bioenergetics, 1857, 1900-1908 (2016)


• Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris
  Md. Ruhul Kuddus, Farhana Rumi, M. Tsutsumi, R. Takahashi, M. Yamano, M. Kamiya, T. Kikukawa, M. Demura, T. Aizawa
  Protein Expression and Purification, 122, 15-22 (2016).


• Lipopolysaccharide-bound structure of the antimicrobial peptide cecropin P1 determined by nuclear magnetic resonance spectroscopy
  M. Baek, M. Kamiya, T. Kushibiki, T. Nakazumi, S. Tomisawa, C. Abe, Y. Kumaki, T. Kikukawa, M. Demura, K. Kawano, T. Aizawa
  Journal of Peptide Science, 22, 214-221 (2016)


• Formation of M-Like Intermediates in Proteorhodopsin in Alkali Solutions (pH > 8.5) Where the Proton Release Occurs First in Contrast to the Sequence at Lower pH
  J. Tamogami, K. Sato, S. Kurokawa, T. Yamada, T. Nara, M. Demura, S. Miyauchi, T. Kikukawa, E. Muneyuki, N. Kamo
  Biochemistry, 55, 1036-1048 (2016) Jan 26. [Epub ahead of print]


• Structure determination of uniformly ¹³C, ¹⁵N labeled protein using qualitative distance restraints from MAS solid-state ¹³C-NMR observed paramagnetic relaxation enhancement
  H. Tamaki, A. Egawa, K. Kido, T. Kameda, M. Kamiya, T. Kikukawa, T. Aizawa, T. Fujiwara, M. Demura
  Journal of Biomolecular NMR, 64, 87-101 (2016) , First online: 04 January 2016

2015

• In vivo fluorescence-correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor
  M. Tsutsumi, H.Muto, S. Myoba, M. Kimoto, A. Kitamura, M. Kamiya, T. Kikukawa, S. Takiya, M. Demura, K. Kawano, M. Kinjo, T. Aizawa
  FEBS Open Bio, 6, 106-125 (2015)


• Characterization of a Cyanobacterial Chloride-Pumping Rhodopsin and Its Conversion into a Proton Pump
  T. Hasemi, T. Kikukawa, N. Kamo, M. Demura
  J. Biol. Chem., 291, 355-362 (2015)


• Structural Basis for Slow Photocycle and Late Proton Release in Acetabularia Rhodopsin I from the Marine Plant, Acetabularia acetabulum, and membrane potential-induced modulation of the photocycle
  M. Furuse, J. Tamogami, T. Hosaka, T. Kikukawa, N. Shinya, M. Hato, N. Ohsawa, S. Y. Kim, K.-H. Jung, M. Demura, S. Miyauchi, N. Kamo, K. Shimono, T. Kimura-Someya, S. Yokoyama, M. Shirouzu
  Acta Crystallographica Section D, 71, 2203-2216 (2015)


• Solution structure of an avirulence protein, AVR-Pia, from Magnaporthe oryzae
  T. Ose, A. Oikawa, Y. Nakamura, K. Maenaka, Y. Higuchi, Y. Satoh, Shiho Fujiwara, M. Demura, T. Sone, M. Kamiya
  Journal of Biomolecular NMR, 63, 229-235 (2015)


• Microbial rhodopsins of Halorubrum species isolated from Ejinoor salt lake in Inner Mongolia of China
  Chaoluomeng, G. Dai, T. Kikukawa, K. Ihara, T. Iwasa
  Photochem Photobiol Sci., 14, 1974-1982 (2015)


• Probing the Cl^--pumping photocycle of pharaonis halorhodopsin: Examinations with bacterioruberin, an intrinsic dye, and membrane potential-induced modulation of the photocycle
  T. Kikukawa, C. Kusakabe, A. Kokubo, T. Tsukamoto, M. Kamiya, T. Aizawa, K. Ihara, N. Kamo, M. Demura
  Biochim. Biophys. Acta - Bioenergetics, 1847, 748-758 (2015)


• Efficient production of a correctly folded mouse a-defensin, cryptdin-4, by refolding during inclusion body solubilization
  S. Tomisawa, Y. Sato, M. Kamiya, Y. Kumaki, T. Kikukawa, K. Kawano, M. Demura, K. Nakamura, T. Ayabe, T. Aizawa
  Protein Expression and Purification, 112, 21-28 (2015)

2014

• The effects of chloride ion binding on the photochemical properties of sensory rhodopsin II from Natronomonas pharaonis
  J. Tamogami, K. Iwano, A. Matsuyama, T. Kikukawa, M. Demura, T. Nara, N. Kamo
  Journal of Photochemistry and Photobiology B: Biology, 141, 192-201 (2014)


• Irreversible Trimer to Monomer Transition of Thermophilic Rhodopsin upon Thermal Stimulation
  T. Tsukamoto, M. Demura, Y. Sudo
  Journal of Physical Chemistry B, 118, 12383-12394 (2014)


• Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide
  T. Kushibiki, M. Kamiya, T. Aizawa, Y. Kumaki, T. Kikukawa, M. Mizuguchi, M. Demura, S.-I. Kawabata, K. Kawano
  Biochim. Biophys. Acta - Proteins and Proteomics, 1844, 527-534 (2014)

2013

• Role of Thr218 in the Light-Driven Anion Pump Halorhodopsin from Natronomonas pharaonis.
  K. Shibasaki, H. Shigemura, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
  Biochemistry, 52, 9257-9268 (2013).


• Synthesis and Characterization of Water-soluble Silk Peptides and Recombinant Silk Protein containing Polyalanine,
  the Integrin Binding Site and Two Glutamic Acids at Each Terminal Site as a Possible Candidate for Use in Bone Repair Materials
  T. Asakura, Y. Suzuki, A. Nagano, D. Knight, M. Kamiya, M. Demura
  Biomacromolecules, 14, 3731-3741 (2013)


• Role of S-palmitoylation on IFITM5 for the interaction with FKBP11 in osteoblast cells
  T. Tsukamoto, X. Li, H. Morita, T. Minowa, T. Aizawa, N. Hanagata, M. Demura
  PLoS One, 8, e75831 (2013)


• Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system
  S. Tomisawa, E. Hojo, Y. Umetsu, S. Ohki, Y. Kato, M. Miyazawa, M. Mizuguchi, M. Kamiya, Y. Kumaki, T. Kikukawa, K. Kawano, M. Demura, T. Aizawa
  AMB Express, 3, 45 (2013)


• A new approach to detect small peptides clearly and sensitively by Western blotting using a vacuum-assisted detection method
  S. Tomisawa, C. Abe, M. Kamiya, T. Kikukawa, M. Demura, K. Kawano, T. Aizawa
  Biophysics, 9, 79-83 (2013)


• Molecular mechanisms of the cytotoxicity of human alpha-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes
  T. Nakamura, T. Aizawa, R. Kariya, S. Okada, M. Demura, K. Kawano, K. Makabe, K. Kuwajima
  J. Biol. Chem., 288, 14408-14416 (2013)


• Physicochemical Studies of Bacteriorhodopsin Reconstituted in Partially Fluorinated Phosphatidylcholine Bilayers
  M. Yoshino, T. Kikukawa, H. Takahashi, T. Takagi, Y. Yokoyama, H. Amii, T. Baba, T. Kanamori, M. Sonoyama
  J. Phys. Chem. B, 117, 5422–5429 (2013)


• Exploring the active site structure of a photoreceptor protein by Raman optical activity
  M. Unno, T. Kikukawa, M. Kumauchi, N. Kamo
  J. Phys. Chem. B, 117, 1321–1325 (2013)


• Thermodynamic Parameters of Anion Binding to Halorhodopsin from Natronomonas pharaonis by Isothermal Titration Calorimetry
  S. Hayashi, J. Tamogami, T. Kikukawa, H. Okamoto, K. Shimono, S. Miyauchi, M. Demura, T. Nara, N. Kamo
  Biophysical Chemistry, 172, 61-67 (2013)


• Salt Bridge in the Conserved His-Asp Cluster in Gloeobacter Rhodopsin Contributes to Trimer Formation
  T. Tsukamoto,T. Kikukawa, T. Kurata, K.H. Jung, N.Kamo, M. Demura
  FEBS Lett., 587, 322-327 (2013)

2012

• The Photo-induced Proton Release in Proteorhodopsin at Low pH: The Possibility of the pKa Decrease of Asp227
  J. Tamogami, T. Kikukawa, T. Nara, K. Shimono, M. Demura, N. Kamo
  Biochemistry, 51, 9290-9301 (2012)


• Influence of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I
  L. Reissig, T. Iwata, T. Kikukawa, M. Demura, N. Kamo, H. Kandori, Y. Sudo
  Biochemistry, 51, 8802-8813 (2012)


• Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation
  Y. Furutani, K. Fujiwara, T. Kimura, T. Kikukawa, M. Demura, H. Kandori
  J.Phys.Chem.Lett., 3, 2964-2969 (2012)


• Structural characterization of a trapped folding intermediate of pyrrolidone carboxyl peptidase from a hyperthermophile
  M. Mizuguchi, M. Takeuchi, Y. Nabeshima, S. Ohki, T. Kouno, T. Aizawa, M. Demura, K. Kawano, K. Yutani
  Biochemistry, 51, 6089-6096 (2012)


• Homo-trimer Formation and Dissociation of pharaonis Halorhodopsin in Detergent System
  T. Tsukamoto, T. Sasaki, K. J. Fujimoto, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
  Biophys. J., 102, 2906-2915 (2012)


• Protein-Bound Water as the Determinant of Asymmetric Functional Conversion between Light-Driven Proton and Chloride Pumps
  K. Muroda, K. Nakashima, M. Shibata, M. Demura, H. Kandori
  Biochemistry, 51, 4677-4684 (2012). [chosen to be highlighted]


• Bone formation by porous chitosan / hydroxyapatite composite with rhBMP-2 on rat calvaria
  N. Harada, H. Kashiwazaki, T. Akazawa, M. Murata, T. Aizawa, M. Demura, J. Tanaka, T. Iizuka, N. Inoue
  Hokkaido J. Dent. Sci., 32, 166-176 (2012)


• Photo-induced Bleaching of Sensory Rhodopsin II (Phoborhodopsin) from Halobacterium salinarum by Hydroxylamine: Identification of the Responsible Intermediates
  J. Tamogami, T. Kikukawa, Y. Ikeda, M. Demura, T. Nara, N. Kamo
  Journal of Photochemistry and Photobiology B: Biology, 106 , 87-94 (2012)

2011

• Porous silk fibroin film as a transparent carrier for cultivated corneal epithelial sheets
  K. Higa, S. Shimmura, N. Takeshima, F. Moro, T. Kawakita, M. Kawashima, M. Demura, J. Shimazaki, T. Asakura, K. Tsubota
  Journal of Biomaterials Science: Polymer Edition, 22, 2261-2276 (2011)


• Expression of salinarum halorhodopsin in Escherichia coli cells: solubilization in the presence of retinal yields the natural state
  Y. Yamashita, T. Kikukawa, T. Tsukamoto, M. Kamiya, T. Aizawa, K. Kawano, S. Miyauchi, N. Kamo, M. Demura
  Biochim. Biophys. Acta, 1808, 2905-2912, (2011)


• NMR analysis of the fibronectin cell-adhesive sequence, Arg-Gly-Asp, in a recombinant silk-like protein and a model peptide
  T. Asakura, H. Nishi, A. Nagano, A. Yoshida, Y. Nakazawa, M. Kamiya, M. Demura
  Biomacromolecules, 12, 3910-3916 (2011)


• Photochemistry of Acetabularia Rhodopsin II from a Marine Plant, Acetabularia acetabulum
  T. Kikukawa, K. Shimono, J. Tamogami, S. Miyauchi, S. Y. Kim, T. Kimura-Someya, M. Shirouzu, K. H. Jung, S. Yokoyama, N. Kamo
  Biochemistry, 50, 8888–8898 (2011)


• Crystal structure of the eukaryotic light-driven proton pumping rhodopsin, Acetabularia rhodopsin II, from marine alga
  T. Wada, K. Shimono, T. Kikukawa, M. Hato, N. Shinya, S. Y. Kim, T. Kimura-Someya, M. Shirouzu, J. Tamogami, S. Miyauchi, K. H. Jung, N. Kamo, S. Yokoyama
  J. Mol. Biol., 422, 986-998 (2011)


• An amino acid residue (S201) in the retinal binding pocket regulates the photoreaction pathway of phoborhodopsin
  G. Dai, Y. Zhang, J. Tamogami, M. Demura, N. Kamo, H. Kandori, T. Iwasa
  Biochemistry, 50, 7177-7183 (2011)


• Sensitive detection of protein-lipid interaction change on bacteriorhodopsin using Dodecyl-β-D-maltoside
  T. Sasaki, M. Demura, N. Kato, Y. Mukai
  Biochemistry, 50, 2283-2290 (2011)


• Spectroscopic Evidence for the Formation of an N Intermediate during the Photocycle of Sensory Rhodopsin II (Phoborhodopsin) from Natronobacterium pharaonis
  Y. Tateishi, T. Abe, J. Tamogami, Y. Nakao, T. Kikukawa, N. Kamo, M Unno
  Biochemistry, 50, 2135–2143 (2011)


• A microbial rhodopsin with a unique retinal composition shows both sensory rhodopsin II and bacteriorhodopsin-like properties
  Y. Sudo, K. Ihara, S. Kobayashi, D. Suzuki, H. Irieda, T. Kikukawa, H. Kandori, M. Homma
  J Biol Chem., 286, 5967-5976 (2011)


• The structure of Physarum polycephalum hemagglutinin I suggests a minimal carbohydrate recognition domain of legume lectin fold
  T. Kouno, N. Watanabe, N. Sakai, T. Nakamura, Y. Nabeshima, M. Morita, M. Mizuguchi, T. Aizawa, M. Demura, T. Imanaka, I. Tanaka, K. Kawano
  J. Mol. Biol., 405, 560-569 (2011)


• Photochemistry of a putative new class of sensory rhodopsin (SRIII) coded by xop2 of Haloarcular marismortui
  Y. Nakao, T. Kikukawa, K. Shimono, J. Tamogami, N. Kimitsuki, T. Nara, M. Unno, K. Ihara, N. Kamo
  J Photochem Photobiol B., 102, 45-54 (2011)

2010

• Development of an injectable chitosan/marine collagen composite gel
  W. Wang, S. Itoh, T. Aizawa, K. Sakai, T. Ohkuma, M. Demura
  Biomedical Materials, 5, 065009 (2010)


• STPR, A 23-amino-acid tandem repeat domain, found in the human function-unknown protein ZNF821
  Y. Nonaka, H. Muto, T. Aizawa, E. Okabe, S. Myoba, T. Yokoyama, S. Saito, F. Tatami, Y. Kumaki, M. Kamiya, T. Kikukawa, M. Mizuguchi, S. Takiya, M. Kinjo, M. Demura, K. Kawano
  Biochemistry, 49, 8367-8375 (2010)


• Polyglutamine tract-binding protein-1 binds to U5-15kD via a continuous 23-residue segment of the C-terminal domain.
  M. Takahashi, M. Mizuguchi, H. Shinoda, T. Aizawa, M.Demura, H. Okazawa, K. Kawano
  Biochim. Biophys. Acta - Proteins and Proteomics, 1804, 1500-1507 (2010)


• Structure-activity relationship of a novel pentapeptide with cancer cell growth-inhibitory activity.
  M. Kamiya, K. Oyauchi, Y. Sato, T. Yokoyama, M. Wang, T. Aizawa, Y. Kumaki, M. Mizuguchi, K. Imai, M. Demura, K. Suzuki, K. Kawano
  J.Peptide Sci., 16, 242-248 (2010)


• The photochemical reaction cycle and photo-induced proton transfer of sensory rhodopsin II (phoborhodopsin) from Halobacterium salinarum
  J. Tamogami, T. Kikukawa, Y. Ikeda, A. Takemura, M. Demura,，N. Kamo
  Biophys. J., 98, 1353-1363 (2010). [Selected as Featured Article; available for free.]


• Photoreaction cycle of phoborhodopsin (sensory rhodopsin II) from Halobacterium salinarum expressed in Escherichia coli
  G. Dai, Y. Ohno, Y. Ikeda, J. Tamogami, T. Kikukawa, N. Kamo, T. Iwasa
  Photochem Photobiol, 86, 571-579 (2010)


• Spectroscopic Studies of a Sensory Rhodopsin I Homologue from the Archaeon Haloarcula vallismortis
  J. Yagasaki, D. Suzuki, K. Ihara, K. Inoue, T. Kikukawa, M. Sakai, M. Fujii, M. Homma, H. Kandori, Y. Sudo
  Biochemistry, 49, 1183–1190 (2010)

2009

• Effect of chloride binding on the thermal trimer-monomer conversion of halorhodopsin in the solubilized system
  T. Sasaki, T. Aizawa, M. Kamiya, T. Kikukawa, K. Kawano, N. Kamo, M. Demura
  Biochemistry, 48, 12089-12095 (2009) Epub; November 18, 2009


• Effect of Molecular Assembly on Photocycle of Reconstituted Bacteriorhodopsin: Significant Blue Shift of the Late M Photointermediate in the Liquid Crystalline Phase
  M. Sonoyama, T. Kikukwa, Y. Yokoyama, M. Demura, N. Kamo, S. Mitaku
  Chem. Lett., 38, 1134-1135 (2009)


• C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity
  Y. Umetsu, T. Aizawa, K. Muto, H. Yamamoto, M. Kamiya, Y. Kumaki, M. Mizuguchi, M. Demura, Y. Hayakawa, K. Kawano
  J. Biol. Chem., 284 , 29625-29634 (2009)


• Electrophoresis of Dyes and Proteins in Poly(Acrylamide) Gel Containing Immobilized Bilayer Membranes
  H. Ishihara, G. Matsuo, T. Sasaki, Y. Saito, M. Demura, K. Tsujii
  Progress in Colloid and Polymer Science, 136, 143-148 (2009)


• Effects of chloride ion binding on the photochemical properties of Salinibacter sensory rhodopsin I
  D. Suzuki, Y. Furutani, K. Inoue, T. Kikukawa, M. Sakai, M. Fujii, H. Kandori, M. Homma, Y. Sudo
  J Mol Biol., 392, 48-62 (2009)


• A novel β-defensin structure: big defensin changes its N-terminal structure to associate with the target membrane
  T. Kouno, M. Mizuguchi, T. Aizawa, H. Shinoda, M. Demura, S. I. Kawabata, K. Kawano
  Biochemistry, 48, 7629-7635 (2009)


• DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
  S. Takiya, S. Saito, T. Yokoyama, D. Matsumoto, T. Aizawa, M. Kamiya, M. Demura, Keiichi Kawano
  J. Biochem., 146, 103-111 (2009) [Epub 2009 Mar 20.]


• Property of Anion Binding Site of pharaonis Halorhodopsin Studied by Ultrafast Pump-Probe Spectroscopy and Low-Temperature FTIR Spectroscopy
  K. Nakashima, T. Nakamura, S. Takeuchi, M. Shibata, M. Demura, T. Tahara, H. Kandori
  J. Phys. Chem., 113, 8429-8434 (2009) [Epub 2009 May 22]


• Polyglutamine tract binding protein-1 is an intrinsically unstructured protein
  M. Takahashi, M. Mizuguchi, H. Shinoda, T. Aizawa, M. Demura, H. Okazawa, K. Kawano
  Biochim. Biophys. Acta - Proteins and Proteomics, 1794, 936-943 (2009) [Epub 2009 Mar 17]


• A Novel Peptide Mediates Aggregation and Migration of Hemocytes From an Insect
  S. Nakatogawa, Y. Oda, M. Kamiya, T. Kamijima, T. Aizawa, K. D. Clark, M. Demura, K. Kawano, M. R. Strand, Y. Hayakawa
  Current Biology, 19, 779-785 (2009) [Epub 2009 Apr 16]


• Reaction dynamics of halorhodopsin studied by time-resolved diffusion method
  K. Inoue, M. Kubo, M. Demura, N. Kamo, M. Terazima
  Biophys. J., 96, 3724-3734 (2009)


• X-ray crystallography and structural stability of digestive lysozyme from cow stomach
  Y. Nonaka, D. Akieda, T. Aizawa, N. Watanabe, M. Kamiya, Y. Kumaki, M. Mizuguchi, T. Kikukawa, M. Demura, M. K. Kawano
  FEBS J., 276, 2192-2200 (2009)


• Role of Arg123 in light-driven anion pump mechanisms of pharaonis halorhodopsin
  M. Kubo, T. Kikukawa, S. Miyauchi, A. Seki, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
  Photochem. Photobiol., 85, 547-555 (2009)


• Halorhodopsin from Natronomonas pharaonis forms a trimer even in the presence of a detergent, dodecyl-beta-D-maltoside
  T. Sasaki, M. Kubo, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
  Photochem. Photobiol., 85, 130-136 (2009) Published Online: Aug 12 2008 9:37AM

2008

• Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death
  T. Kamijima, A. Ohmura; T. Sato, K. Akimoto M. Itabashi,　M. Mizuguchi, M. Kamiya, T. Kikukawa, T. Aizawa, M. Takahashi, K. Kawano, M. Demura
  Biochem.Biophys.Res.Commun., 376, 211-214 (2008) Epub 2008 Sep 5.


• Ultrafast Pump-Probe Study of the Primary Photoreaction Process in pharaonis Halorhodopsin: Halide-Ion Dependence and Isomerization Dynamics
  T. Nakamura, S. Takeuchi, M. Shibata, M. Demura, H. Kandori, T. Tahara
  Journal of Physical Chemistry B, 112, 12795-12800 (2008)


• A Novel beta-defensin Structure: a Strategy of Big Defensin for a Resistance by Gram-positive Bacteria
  T. Kouno, N.Fujitani, M. Mizuguchi, T. Osaki, S.-I. Nishimura, S.-i. Kawabata, T. Aizawa, M. Demura, K. Nitta, K. Kawano
  Biochemistry, 47, 10611-10619 (2008)


• The structure of S100A11 fragment explains a local structural change induced by phosphorylation
  T. Kouno, M. Mizuguchi, M. Sakaguchi, E. Makino, Y. Mori, H. Shinoda, T. Aizawa, M. Demura, N.H. Huh, K. Kawano
  J Pept Sci., 14, 1129-1138 (2008)


• Enhanced nerve regeneration through a bilayered chitosan tube: The effect of introduction of glycine spacer into the CYIGSR sequence
  W. Wang, S. Itoh, A. Matsuda, T. Aizawa, M. Demura, S. Ichinose, K. S., J. Tanaka
  J Biomed Mater Res A, 85A, 919-928 (2008)


• Structural Properties of the DNA-Bound Form of a Novel Tandem Repeat DNA-Binding Domain, STPR
  S. Saito, T. Yokoyama, T. Aizawa, K. Kawaguchi, T. Yamaki, D. Matsumoto, T. Kamijima, M. Kamiya, Y. Kumaki, M. Mizuguchi, S. Takiya, M. Demura, K. Kawano,
  Proteins, 72, 414-426 (2008)


• Spontaneous asparaginyl deamidation of canine milk lysozyme under mild conditions
  Y. Nonaka, T. Aizawa, D. Akieda, M. Yasui, M. Watanabe, N. Watanabe, I. Tanaka, M. Kamiya, M. Mizuguchi, M. Demura, K. Kawano
  Proteins, 72, 313-322 (2008)


• Unfolding and aggregation of transthyretin by the truncation of 50 N-terminal amino acids.
  M. Mizuguchi, A. Hayashi, M. Takeuchi, M. Dobashi, Y. Mori, H. Shinoda, T. Aizawa, M. Demura, K. Kawano
  Proteins, 72, 261-269 (2008)

2007

• The Structure of a Novel Insect Peptide Explains Its Ca(2+) Channel Blocking and Antifungal Activities
  T. Kouno, M. Mizuguchi, H. Tanaka, P. Yang, Y. Mori, H. Shinoda, K. Unoki, T. Aizawa, M. Demura, K. Suzuki, K. Kawano
  Biochemistry, 46, 13733-13741 (2007)


• Heterologous expression of pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl^- pump activity
  A. Seki, S. Miyauchi, S. Hayashi, T. Kikukawa, M. Kubo, M. Demura, V. Ganapathy, N. Kamo
  Biophys. J., 92, 2559-2569 (2007)


• The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an inhibitory cystine-knot motif
  N. Fujitani, T. Kouno, T. Nakahara, K. Takaya, T. Osaki, S. Kawabata, M. Mizuguchi, T. Aizawa, M. Demura, S. Nishimura, K. Kawano
  J. Pept. Sci., 13, 269-279 (2007)


• Equilibrium and Kinetics of the Folding and Unfolding of Canine Milk Lysozyme
  H. Nakatani, K. Maki,K. Saeki, T. Aizawa, M. Demura, K. Kawano, S. Tomoda, K. Kuwajima
  Biochemistry, 46, 5238 - 5251 (2007)


• Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to Bind the Transducer: Cl^- Circulation within the Extracellular Channel
  C. Hasegawa, T. Kikukawa, S. Miyauchi, A. Seki,Y. Sudo, M. Kubo, M. Demura, N. Kamo
  Photochem. Photobiol., 83, 293-302　（2007）


• Structural Approach to a Novel Tandem Repeat DNA-Binding Domain, STPR, by CD and NMR
  S. Saito, T. Aizawa, K. Kawaguchi, T. Yamaki, D. Matsumoto, M. Kamiya, Y. Kumaki, M. Mizuguchi, S. Takiya, M. Demura, K. Kawano
  Biochemistry, 46, 1703-1713 (2007)


• Destabilization of Transthyretin by Pathogenic Mutations in the DE loop
  M. Takeuchi, M. Mizuguchi, T. Kouno, Y. Shinohara, T. Aizawa, M. Demura, Y. Mori, H. Shinoda, K. Kawano
  PROTEINS: Structure, Function, and Bioinformatics, 66, 716-725 (2007)

2006

• Deprotonation of Glu234 during the photocycle of Natronomonas pharaonis halorhodopsin
  M. Shibata, Y. Saito, M. Demura, H. Kandori
  Chemical Physics Letters, 432, 545-547 (2006)


• Differential Scanning Calorimetry of a Metalloprotein under Controlled Metal-Ion Activity
  M. Yasui, T. Miyahara, T. Aizawa, M. Demura, K. Nitta
  Protein J., 25, 475-482 (2006)


• Fractionation of silk fibroin hydrolysate and its protective function of hydrogen peroxide toxicity
  J.-H. Yeo, K.-G. Lee, H.-Y. Kweon, S.-O. Woo, S.-M. Han, S.-S. Kim, M. Demura
  J. Appl. Polym. Sci., 102, 772-776 (2006)


• N-Terminal Mutational Analysis of the Interaction between Growth-Blocking Peptide (GBP) and Receptor of Insect Immune Cells
  S. Watanabe, M. Tada, T. Aizawa, M. Yoshida, T. Sugaya, M. Taguchi, T. Kouno, T. Nakamura, M. Mizuguchi, M. Demura, Y. Hayakawa, K. Kawano
  Protein & Peptide Lett., 13, 815-822 (2006)


• A Novel N14Y Mutation in Connexin26 in KID Syndrome ? Analyses of Altered Gap Junctional Communication and Molecular Structure of N-Terminus of Mutated Connexin26
  K. Arita, M. Akiyama, T. Aizawa, Y. Umetsu, I. Segawa, M. Goto, D. Sawamura, M. Demura, K. Kawano, H. Shimizu
  Am. J. Pathology, 169, 416-423 (2006)


• Internal Water Molecules of the Proton-Pumping Halorhodopsin in the Presence of Azide
  N. Muneda, M. Shibata, M. Demura, H. Kandori
  J. Am. Chem. Soc., 128, 6294-6295 (2006)


• Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein Transgenic Mouse Head
  M. Tada, Y. Shinohara, I. Kato, K. Hiraga, T. Aizawa, M. Demura,Y. Mori, H. Shinoda, M. Mizuguchi, K. Kawano
  Acta Histochemica et Cytochemica, 39, 31-34 (2006)


• NMR insight of structural stability and folding of calcium-binding lysozyme
  M. Demura
  Modern Magnetic Resonance, Part I, 497-501 (2006)

2005

• Effects of the Stabilization of the Molten Globule State on the Folding Mechanism of alpha-Lactalbumin: A Study of a Chimera of Bovine and Human alpha-Lactalbumin
  M. Mizuguchi, A. Matsuura, Y. Nabeshima, K. Masaki, M. Watanabe, T.Aizawa, M. Demura, K. Nitta, Y. Mori, H. Shinoda K. Kawano
  Proteins, 61, 356-365 (2005)


• Disassembling and bleaching of chloride-free pharaonis halorhodopsin by octyl-beta-glucoside
  M. Kubo, M. Sato, T. Aizawa, C. Kojima, N. Kamo, M. Mizuguchi, K. Kawano, M. Demura
  Biochemistry, 44, 12923-12931 (2005)


• Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule in the Chloride Pump of Natronobacterium pharaonis
  M. Shibata , N. Muneda , T. Sasaki , K. Shimono, N. Kamo, M. Demura, H. Kandori
  Biochemistry, 44, 12279-12286 (2005)


• Photobleaching of bacteriorhodopsin solubilized with Triton X-100
  T. Sasaki, M. Sonoyama, M. Demura, S. Mitaku
  Photochemistry and Photobiology, 81, 1131-1137(2005)


• Role of Putative Anion-Binding Sites in Cytoplasmic and Extracellular Channels of Natronomonas pharaonis Halorhodopsin
  M. Sato, M. Kubo, T. Aizawa, N. Kamo, T. Kikukawa, K. Nitta, M.Demura
  Biochemistry, 44, 4775-4784 (2005)


• Interaction of dopamine and acetylcholine with an amphiphilic resorcinarene receptor in aqueous micelle system
  M. Demura, T. Yoshida, T. Hirokawa, Y. Kumaki, T. Aizawa, K. Nitta, I.Bitter, K. Toth
  Bioorganic & Medicinal Chemistry Letters, 15, 1367-1370 (2005)

2004

• The Gly-Gly Linker Region of the Insect Cytokine Growth Blocking Peptide(GBP) is Essential for Activity
  M. Yoshida, T. Aizawa, T. Nakamura, K. Shitara, Y. Hayakawa, K. Matsubara, K. Miura, T. Kouno, K. D. Clark,M. R. Strand, M. Mizuguchi, M. Demura, K. Nitta, K. Kawano
  J. Biol. Chem., 279, 51331-51337 (2004)


• Volumetric behavior of the molten globule state of canine milk lysozyme
  M. Watanabe, Y. Kobashigawa, T. Aizawa, M. Demura, K. Nitta
  Protein Pept Lett., 11, 325-330 (2004).


• Effect of Hydrostatic Pressure on Conformational Changes of Canine Milk Lysozyme between the Native, Molten Globule, and Unfolded States
  M. Watanabe, T. Aizawa, M. Demura, K. Nitta
  Biochim. Biophys. Acta, 1702, 129-136 (2004)


• Internal Water Molecules of Light-Driven Chloride Pump Proteins
  M. Shibata, N. Muneda, K. Ihara, T. Sasaki, M. Demura, H. Kandori
  Chem. Phys. Lett., 392, 330-333 (2004)


• A Non-Native alpha-Helix Is Formed in the beta-Sheet Region of the Molten Globule State of Canine Milk Lysozyme
  M. Watanabe, Y. Kobashigawa, T. Aizawa, M. Demura, K. Nitta
  Protein J., 23, 335-342 (2004)

2003

• Roles of Ser130 and Thr126 in Chloride Binding and Photocycle of pharaonis Halorhodopsin
  M. Sato, T. Kikukawa, T. Araiso, H. Okita, K. Shimono, N. Kamo, M. Demura, K. Nitta
  J. Biochem., 134, 151-158 (2003)


• Ser-130 of Natronobacterium pharaonis Halorhodopsin Is Important for the Chloride Binding
  M. Sato, T. Kikukawa, T. Araiso, H. Okita, K. Shimono, N. Kamo, M. Demura, K. Nitta
  Biophys. Chem., 104, 209-216 (2003)
• Structure Determination and Conformational Change Induced by Tyrosine Phosphorylation of the N-terminal Domain of the alpha-Chain of Pig Gastric H⁺/K⁺-ATPase
  N. Fujitani, M. Kanagawa, T. Aizawa, T. Ohkubo, S. Kaya, M. Demura, K. Kawano, K. Taniguchi, K. Nitta
  Biochem. Biophys. Res. Commun., 300, 223-229 (2003)

2002

• Stabilization of protein by replacement of a fluctuating loop: structural analysis of a chimera of bovine alpha-lactalbumin and equine lysozyme
  M. Tada, Y. Kobashigawa, M. Mizuguchi, K. Miura, T. Kouno, Y. Kumaki, M. Demura, K. Nitta, K. Kawano
  Biochemistry, 41, 13807-13813 (2002)


• Equilibrium and Kinetic Folding of Hen Egg-white Lysozyme under Acidic Conditions
  K. Sasahara, M. Demura, K. Nitta
  Proteins, 49, 472-82 (2002)


• Effects of a Helix Stabilization on the Folding Machanism of alpha-lactalbumin
  M.Mizuguchi, Y. Kobashigawa, Y. Kumaki, M. Demura, K. Kawano, K. Nitta
  Proteins, 49,95-103 (2002)


• Structural Analysis of an Insect Lysozyme Exhibiting Catalytic Efficiency at Low Temperatures
  A. Matsuura, M. Yao, T. Aizawa, N. Koganesawa, K. Masaki, M. Miyazawa, M. Demura, I. Tanaka, K. Kawano, K. Nitta
  Biochemistry, 41, 12086 - 12092 (2002)


• Production and Characterization of Recombinant Tachycitin, the Chitin-Binding Protein
  T. Suetake, T. Aizawa, N. Koganesawa, T. Osaki, Y. Kobashigawa, M. Demura, S. Kawabata, K. Kawano, S. Tsuda, K. Nitta
  Protein Engineering, 15, 763-769(2002)


• Expression and purification of small cytokine growth-blocking peptide(GBP) from armyworm Pseudaletia separata by the optimized fermentation metnod using methylotrophic yeast Pichia pastoris
  N. Koganesawa, T.Aizawa, H.Shimojo, K.Miura, A.Ohnishi, M. Demura, Y.Hayakawa, K.Nitta, K.Kawano
  Protein Expression and Purification, 25, 416-425(2002)


• Structure of the antimicrobial peptide Tachystatin A
  N. Fujitani, S. Kawabata, T. Osaki, Y. Kumaki, M. Demura. K. Nitta, K. Kawano
  J. Biol. Chem., 277, 23651-23657 (2002)


• Stopped-Flow Analysis on Anion Binding to Blue-form Halorhodopsin from Natronobacterium pharaonis: Comparison with Anion-Uptake Process during Photocycle
  M. Sato, T.Kanamori, N. Kamo, M. Demura, K. Nitta
  Biochemistry, 41, 2452-2458 (2002)


• Water-Induced Crystallization of Hydrogels
  T. Miyazaki, T. Kaneko, J.P. Gong, Y. Osada, M. Demura, M. Suzuki
  Langmuir, 18,965-967 (2002)


• Solution Structure of Sialyl Lewis X mimic Studied by Two-dimensional NMR
  M. Demura, M. Noda, T. Kajimoto, T. Uchiyama, K. Umemoto, C. H. Wong, T. Asakura
  J. Mol. Struc., 602, 215-222 (2002)


• Functional Silk Proteins: Molecular Structure and Application to Biomaterials
  M. Demura, J. H. Yeo, K.G. Lee, Y.W. Lee
  Int. J. Industrial Entomology, 4, 1-4 (2002)

2001

• Energetics of three-state unfolding of a protein: canine milk lysozyme
  T. Koshiba, Y. Kobashigawa, M. Demura, K. Nitta
  Protein Eng., 14, 967-974 (2001)


• Structure and activity of the insect cytokine growth-blocking peptide. Essential regions for mitogenic and hemocyte-stimulating activities are separate.
  T. Aizawa, Y. Hayakawa, A. Ohnishi, N. Fujitani, K. D. Clark, M.R. Strand, K. Miura, N. Koganesawa, Y. Kumaki, M. Demura, K. Nitta, K. Kawano
  J. Biol. Chem., 276, 31813-31818 (2001)


• Carbohydrate Recognition of Gramicidin S Analogs in Aqueous Medium
  T. Niidome, H. Murakami, M. Kawazoe, T. Hatakeyama, Y. Kobashigawa, M. Matsushita, Y. Kumaki, M. Demura, K. Nitta, H. Aoyagi
  Bioorganic Medicinal Chemistry Letters, 11,1893-1896(2001)


• Binding Feature of Mastoparan Inducing Phase Change of Lipid Bilayers
  M. Demura
  Seibutsubutsuri, 41, 20-23 (2001)
• Assignment of ¹H, ¹³C, and ¹⁵N Resonances of Canine Milk Lysozyme
  Y. Kobashigawa, K. Miura, M. Demura, N. Nemoto, T. Koshiba, K. Nitta, S. Tsuda
  J. Biomol. NMR, 19, 387-388 (2001)


• Oxidative Folding of Human Lysozyme: the Effects of the Loss of Two Disulfide Bonds and the Introduction of a Calcium-Binding Site
  Y. Kurokawa, N. Koganesawa, Y. Kobashigawa, T.Koshiba, M. Demura, K. Nitta
  J. Protein Chem., 20, 293-303 (2001)


• Interaction of Mastoparan with Membranes Studied by ¹H-NMR in Detergent Micelles and by Solid State ²H-NMR and ¹⁵N-NMR in Oriented Lipid Bilayers
  Y. Hori, M. Demura, M. Iwadate, A.S. Ulrich, T. Niidome, H. Aoyagi, T. Asakura
  Eur. J. Biochem., 267, 302-309 (2001)

2000

• Hydrogen Excahnge Study of Canine Milk Lysozyme: Stabilization Mechamism of the Molten Globule
  Y. Kobashigawa, M. Demura, T. Koshiba, Y. Kumaki, K. Kuwajima, K. Nitta
  Proteins, 40, 579-589 (2000)


• Solid-state NMR determination of the secondary structure of Samia cynthia ricini silk
  J.D.van Beek, L. Beaulieu, H. Schafer, M. Demura, T. Asakura, B.H. Meier
  Nature, 405, 1077-1079 (2000)


• Partially Unfolded Equilibrium State of Hen Lysozyme Studied by Circular Dichroism Spectroscopy
  K. Sasahara, M. Demura, K. Nitta
  Biochemistry, 39, 6475-6482 (2000)


• Structure and Thermodynamics of the Extraordinary Stable Molten Globule state of Canine Milk Lysozyme
  T. Koshiba, M. Yao, Y. Kobashigawa, M. Demura, A. Nakagawa, I. Tanaka, K. Kuwajima, K. Nitta
  Biochemistry, 39, 3248-3257 (2000)


• Local and Long-range Interactions in the Molten Globule State: a study of chimeric proteins of bovine and human alpha-lactalbumin
  M. Mizuguchi, K. Masaki, M. Demura, K. Nitta
  J. Mol. Biol., 298, 985-995 (2000)


• Biosynthesis of L-alanine, a major amino acid of fibroin in Samia cynthia ricini
  M. Osanai, M Okudaira, J. Naito, M, Demura, T. Asakura
  Insect Biochemistry and Molecular Biology, 30, 225-232 (2000)

1999

• Orientational behavior of phospholipid membranes with mastoparan studied by ³¹P solid state NMR
  Y. Hori, M. Demura, T. Niidome, H. Aoyagi and T. Asakura
  FEBS Lett., 455, 228-232 (1999)
• Structural analysis of silk with ¹³C NMR chemical shift contour plots
  T. Asakura, M. Iwadate, M. Demura, and M.P. Williamson
  Int. J. Biol. Macromol., 24, 167-171 (1999)

1998

• Structure of Bombyx mori Silk Fibroin based on Solid State NMR Orientational Constraints and Fiber Diffraction Unit Cell Parameters
  M. Demura, M. Minami, T. Asakura, and T. A. Cross
  J. Am. Chem. Soc., 120, 1300-1308 (1998)


• Structure of Uniaxially Aligned ¹³C Labeled Silk Fibroin Fibers with Solid State ¹³C-NMR
  M. Demura, Y. Yamazaki, T. Asakura and K. Ogawa
  J. Molecular Structure, 441, 155-163 (1998)