Articles

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2024

Research Article

Sumire Ishida-Ishihara^#, Kan Yaguchi^#, Sena Miura^#, Ryoto Nomura, QiJiao Wang, Koya Yoshizawa, Kimino Sato, Guang Yang, Krisztina Veszelyi, Gabor Banhegyi, Eva Margittai, and Ryota Uehara* (*corresponding author; ^# equally first author)

Fragility of ER homeostatic regulation underlies haploid instability in human somatic cells

Journal of Biological Chemistry (2024) In press プレスリリース

 

Kan Yaguchi#, Daiki Saito#, Triveni Menon, Akira Matsura, Miyu Hosono, Takeomi Mizutani, Tomoya Kotani, Sreelaja Nair*, Ryota Uehara* (#equally first authors; *co-corresponding author)

Haploidy-linked cell proliferation defects limit larval growth in Zebrafish

Open Biology (2024) In press  プレスリリース

 

Saugata Sahu, Koya Yoshizawa, Takahiro Yamamoto, Ryota Uehara, Nobuyuki Tamaoki

Photoswitchable Auxin-Inducible Degron System for Conditional Protein Degradation with Spatiotemporal Resolution

Journal of the American Chemical Society (2024)link

 

Kazuya Matsuo,   Ryota Uehara,  Takashi Kikukawa,  Tomonori Waku,  Akio Kobori,  and  Nobuyuki Tamaoki

Spatiotemporal regulation of CENP-E-guided chromosomes using a fast-relaxing arylazopyrazole photoswitch

Chemical Communications (2024) link

 

Method paper

Akira Matsura, Shota Hiruma, Nobuyuki Tamaoki, and Ryota Uehara* (*corresponding author)

Photochemical control of cell division using a photoswitchable CENP-E inhibitor

Methods in Molecular Biology (2025) 2872: 101-113 Link

 

Koya Yoshizawa, and Ryota Uehara* (*corresponding author)

Comparative pharmacological analysis of mitotic inhibitors using isogenic ploidy series of HAP1 cells 

Methods in Molecular Biology (2025) 2872: 207-219 Link

2023

Research Article

Takahiro Yamamoto, and  Ryota Uehara* (*corresponding author)

Cell shape instability during cytokinesis in tetraploid HCT116 cells

Biochemical and Biophysical Research Communications (2023) In press

 

Koya Yoshizawa, Akira Matsura, Masaya Shimada, Sumire Ishida-Ishihara, Fuyu Sato, Takahiro Yamamoto, Kan Yaguchi, Eiji Kawamoto, Taruho Kuroda, Kazuya Matsuo, Nobuyuki Tamaoki, Ryuichi Sakai, Yasuhito Shimada, Mithilesh Mishra, and Ryota Uehara* (*corresponding author)

Tetraploidy-linked sensitization to CENP-E inhibition in human cells

Molecular Oncology (2023)17: 1148-1166

プレスリリース　2023ハイライトに採用

2022

Research Article

Tomoko Kamasaki, Ryota Uehara, Yasuyuki Fujita

Ultrastructural Characteristics of Finger-Like Membrane Protrusions in Cell Competition

Microscopy (2022) in press

 

Takafumi Kamada, Kohei Otomo, Takashi Murata, Kaito Nakata, Shota Hiruma, Ryota Uehara, Mitsuyasu Hasebe, Tomomi Nemoto

Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy

Scientific Reports (2022) 12, 809

2021

Research Article

Kosuke Nagata, Ken-ichi Bajo, Hideyuki Mitomo, Ryosuke Fujita, Ryota Uehara, Kuniharu Ijiro, Hisayoshi Yurimoto

Visualization of DNA replication in single chromosome by stable isotope labeling

Cell Structure and Function (2021) 46:95-101

 

Tomoko Kamasaki, Yumi Miyazaki, Susumu Ishikawa, Kazuya Hoshiba, Keisuke, Kuromiya, Nobuyuki Tanimura, Yusuke Mori, Motosuke Tsutsumi, Tomomi Nemoto, Ryota Uehara, Shiro Suetsugu, Toshiki Itoh, and Yasuyuki Fujita

FBP17-mediated finger-like membrane protrusions in cell competition between normal and RasV12-transformed cells

iScience (2021) 24:102994

 

Guang Yang, Shota Hiruma, Akira Kitamura, Masataka Kinjo, Mithilesh Mishra, and Ryota Uehara* (*corresponding author)

Molecular basis of functional exchangeability between ezrin and other actin-membrane associated proteins during cytokinesis

Experimental Cell Research (2021) 403(2):112600

 

Kan Yaguchi, Kimino Sato, Koya Yoshizawa, Daisuke Mikami, Kohei Yuyama, Yasuyuki Igarashi, Gabor Banhegyi, Eva Margittai, and Ryota Uehara* (*corresponding author)

Mevalonate pathway-mediated ER homeostasis is required for haploid stability in human somatic cells

Cell Structure and Function (2021) 46:1-9

 

2020

Research Article

Koya Yoshizawa, Kan Yaguchi, and Ryota Uehara* (*corresponding author)

Uncoupling of DNA replication and centrosome duplication cycles is a primary cause of haploid instability in mammalian somatic cells

Frontiers in Cell and Developmental Biology (2020) 8:721 https://doi.org/10.3389/fcell.2020.00721

 

Noushaba Mafy, Kazuya Matsuo, Shota Hiruma, Ryota Uehara*, and Nobuyuki Tamaoki* (*co-corresponding author)

2018

Research Article

 

Yaguchi, K., Yamamoto, T., Matsui, R., Shimada, M., Shibanuma, A., Kamimura, K., Koda, T., and Uehara, R.* (*corresponding author)

Tetraploidy-Associated Centrosome Overduplication in Mouse Early Embryos

Communicative & Integrative Biology (2018) e1526605-1-e1526605-4 [Full text (via HUSCAP)]

Yaguchi, K.^#, Yamamoto, T.^#, Shimada, M.^#, Sugimoto, R.^#, Nakamura, K., Ayabe, T., and Uehara, R.* (*corresponding author; ^# equally contributed)

Ploidy-dependent change in cyclin D2 expression and sensitization to cdk4/6 inhibition in human somatic haploid cells

Biochemical and Biophysical Research Communications (2018) 504(1):231-237 [Journal site]

 

Yaguchi, K., Yamamoto, T.^#, Matsui, R.^#, Tsukada, Y., Shibanuma, A., Kamimura, K., Koda, T., and Uehara, R.* (*corresponding author; ^# equally contributed)

Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals

Journal of Cell Biology (2018) 217: 2463-2483 [Journal site] [Press release][Full text (via HUSCAP)]

 

2017

Research Article

Hiruma, S., Kamasaki, T., Otomo, K., Nemoto, T., and Uehara, R.* (*corresponding author)

Dynamics and function of ERM proteins during cytokinesis in human cells

FEBS Letters 591: 3296-3309(2017) Full text

Preprint

Kan Yaguchi, Ryo Matsui, Takahiro Yamamoto, Yuki Tsukada, Atsuko Shibanuma, Keiko Kamimura, Toshiaki Koda, and Ryota Uehara* (*corresponding author)

Uncoordinated centrosome duplication cycle underlies the instability of non-diploid states in mammalian somatic cells

BioRχiv doi: https://doi.org/10.1101/194746

2016

Research Article

Uehara, R.*, Kamasaki, T., Hiruma, S., Poser, I., Yoda, K., Yajima, J., Gerlich, D.W., and Goshima, G. (*corresponding author)

Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission.

Molecular Biology of the Cell  1; 27; 812-827 (2016)

Full text (via HUSCAP)

2015　以前

Research Article

Uehara, R.*, Tsukada, Y., Kamasaki, T., Poser, I., Yoda, K., Gerlich, D.W., and Goshima, G.* (*co-corresponding authors)

Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase.

Journal of Cell Biology 202:623-636 (2013) (Article)

 

Nakaoka, Y., Miki, T., Fujioka, R., Uehara, R., Tomioka, A., Obuse, C., Kubo, M., Hiwatashi, Y., and Goshima, G.

An inducible RNAi system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation.

Plant Cell  24:1478-1493 (2012)

 

Uehara, R. and Goshima, G.

Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase.

Journal of Cell Biology 191: 259-267 (2010)

 

Uehara, R., Goshima, G., Mabuchi, I., Vale, R.D., Spudich, J. A., Griffis, E. R.

Determinants of myosin II cortical localization during cytokinesis.

Current Biology 20: 1080-1085 (2010)

 

Uehara, R., Nozawa, R., Tomioka, A., Petry, S., Vale, R., Obuse, C., and Goshima, G.

The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.

Proc Natl Acad Sci USA 106: 6998-7003 (2009)

 

Uehara, R., Hosoya, H., and Mabuchi, I.

In vivo phosphorylation of regulatory light chain of myosin II in sea urchin eggs and its role in controlling myosin localization and function during cytokinesis.

Cell Motility and Cytoskelton 65: 100-115 (2008)