著書（解説・総説等）_その２

 
研究発表論文（著書（解説・総説等）、原著論文、その他）　　　 　出村　誠　

著書（解説・総説等）
31. 出村誠、シルクと持続可能な開発目標(SDGs)、繊維機械学会誌『せんい』, Vol.73 (2020), 印刷中

30. 出村誠・朝倉哲郎、タンパク質、『基礎高分子科学』改訂版・９章 生体高分子、東京化学同人、2020

29. 出村誠、カイコ絹糸の模倣からイノベーションへ, 特集 生物機能模倣技術, アグリバイオ, vol.1, 235-239 (2017)

28. 出村誠、光のエネルギー利用27 ハロロドプシン『光と生命の事典』（日本光生物学協会「光と生命の事典」編集委員会 編）朝倉書店 2016.2.25　

27. Takashi Kikukawa, Naoki Kamo and Makoto Demura, 
Photochemistry of Halorhodopsin in Optogenetics (Eds. Hideki Kandori, Hiromu Yawo, Amane Koizumi) 
Springer 2015, pp47-62.

26. 出村誠、菊川峰志、加茂直樹　ハロロドプシンの構造と分子機能解析
　　『オプトジェネティクス（光遺伝学）-光工学と遺伝子による行動制御技術の最前線-』p24-33,エヌ・ティー・エス(2013) 

25. 相沢智康、出村誠、河野敬一　鱗翅目昆虫で働く生体防御タンパク質の構造生物学, 蚕糸・昆虫バイオテック, 81, 115-123 (2012)

24. T. Kikukawa, J. Tamogami, K. Shimono, M. Demura, T. Nara, N. Kamo
Photo-induced Proton Transfers of Microbial Rhodopsins in Photochemistry, InTech (ISBN 979-953-307-364-3) 2011

23. 出村誠 ペプチドの機能と構造：最小へリックスの暗号、『広がるNMRの世界 -40人の研究者からの熱いメッセージ-』朝倉哲郎編、コロナ社（2011）

22. M. Mizuguchi, T. Aizawa, K. Kawano, M. Demura, 
NMR studies of protein folding: Folding studies of calcium-binding lysozyme and alpha-lactalbumin, 
Annual Reports on NMR Spectroscopy, 65, 2009, 53-76. [URL]

21. 出村誠、神谷昌克、相沢智康　光イオンポンプ『基礎から学ぶ構造生物学』河野・田之倉編　共立出版　150-156, 2008.3

20. 出村誠、光で機能するレチナール膜タンパク質のナノ構造『バイオとナノの融合I新生命科学の基礎』北海道大学出版会　141-152,2007

19. 出村誠・朝倉哲郎、タンパク質、『基礎高分子科学』・８章 生体高分子、東京化学同人、401-417, 2006

18. M. Demura, NMR insight of structural stability and folding of calcium-
binding lysozyme in Modern Magnetic Resonance, Part I Application in Chemistry, 
Biological and Marine Sciences, Springers, 493-497 (2006).

17. M. Demura, J. H. Yeo, K.G. Lee and Y.W. Lee, Functional Silk Proteins: Molecular 
Structure and Application to Biomaterials, Int. J. Industrial Entomology, 4, 1-4 (2002).

16. 出村誠、FORTRANプログラムの作成と実行、生物工学実験書（改訂版）、培風館、439-449, 2002.

15. 出村誠、UNIX言語とファイルの扱い方、生物工学実験書（改訂版）、培風館、427-438, 2002.

14. 出村誠、脂質二重層の相変化を誘起するマストパランの結合様式、生物物理,  41, 20-23, 2001.

13. T. Asakura, M. Demura, H. Yoshimizu and N. Nishikawa, Fibrous Proteins in Solid State NMR 
of Polymers (I. Ando and T. Asakura Eds.) Elsevier, 853-890, 1998.

12. T. Asakura and M. Demura, Oriented Polymers in Solid State NMR of Polymers (I. Ando and T. 
Asakura Eds.) Elsevier, 307-326, 1998.

11. 出村誠、21世紀の核磁気共鳴NMR構造解析-超電導磁石の開発と新測定法-、化学、化学同人、53, 67-68, 1998.

10. 朝倉哲郎、出村誠、シルク研究の新展開、高分子、47, 390-393, 1998.

 9. S. Kurosawa, D. B. Radloff, N. Minoura, J. Miyake and M. Demura, Detection of fluorinated 
gases using piezoelectric quartz crystal coated with plasma-polymerized styrene derivatives, in 
Microsim II Simulation and Design of Microsystem and Microstructures (Eds. by R. A. Adey and 
Ph. Renaud) Computational Mechanics Publications, Southampton, UK, 1997, pp.53-58.

 8.  T. Asakura, M. Demura and N. Nishikawa, Structural Analysis of Oriented 
Polymers by Solid State NMR, Annual Reports on NMR Spectroscopy, Academic Press, 34, 301-346, 1997.

 7. 朝倉哲郎、出村誠、絹生産設計、新繊維科学ーニューフロンティアへの挑戦ー、通商産業調査会, p.475-479, 1995.

 6. T. Asakura, M. Demura and T. Hayashi , 13C NMR Assignments of Polyolefins 
and Olefine Copolymers Based on the 13C NMR Chemical Shift Calculation and 2D 
INADEQUATE NMR, Annual Reports on NMR Spectroscopy, Academic Press, 1994, 29, 325-404.

 5. T. Asakura, M. Demura, A. Uyama, K. Ogawa, K. Ogawa, L. K. 
Nicholson and Timothy A. Cross, NMR Characterization of Silk Proteins, ACS Symposium 
Series; Silks; Biology, Structure, Properties, Gentics, American Chemical Society Books 
Department,1994, 148-154.  

 4. 出村誠、朝倉哲郎、生体高分子, 「高分子材料の機器分析事例集」、技術情報協会, 1994,  229-265.

 3. 朝倉哲郎、出村誠、絹の新しい利用とカイコ体外での生産、高分子加工, 41, 442-446, 1992

 2. 朝倉哲郎、出村誠、グルコースセンサーへの不織布の応用、バイオインダストリｰ、7, 676-682, 1990.

 1. 朝倉哲郎、出村誠、シルクフィブロインの構造特性と酵素センサー膜への利用、繊維学会誌、45, P252-P257､1989.



研究発表論文（原著論文、著書（解説・総説等）、その他）　　　 　出村　誠　

原著論文
185. Azusa Iizuka, Kousuke Kajimoto, Tomotsumi Fujisawa, Takashi Tsukamoto, Tomoyasu Aizawa, Naoki Kamo, Kwang-Hwan Jung, Masashi Unno, Makoto Demura, Takashi Kikukawa
Functional importance of the oligomer formation of the cyanobacterial H+ pump Gloeobacter rhodopsin
Scientific Reports, 9, 10711 (2019)

184. Takatoshi Hasemi, Takashi Kikukawaa, Yumi Watanabe, Tomoyasu Aizawa, Seiji Miyauchi, Naoki Kamo, and Makoto Demura
Photochemical study of a cyanobacterial chloride-ion pumping rhodopsin
Biochim. Biophys. Acta - Bioenergetics, 1860, 136-146 (2019)

183.Takashi Tsukamoto, Chihiro Kikuchi, Hiromu Suzuki, Tomoyasu Aizawa,Takashi Kikukawa & Makoto Demura, 
Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl− concentration, 
Scientific Reports, 8:13445 (2018).

182. Gang Dai, Xiong Geng, Chaoluomeng, J. Tamogami, T. Kikukawa, M. Demura, N. Kamo, T. Iwasa
Photocycle of Sensory Rhodopsin II from Halobacterium salinarum (HsSRII): Mutation of D103 Accelerates M Decay and Changes the Decay Pathway of a 13‐cis O‐like Species
Photochemistry and Photobiology, 94, 705–714 (2018)

181. J. Tamogami, T. Kikukawa, K. Ohkawa, N. Ohsawa, T. Nara, M. Demura, S. Miyauchi, T. Kimura-Someya, M. Shirouzu, S. Yokoyama, K. Shimono, N. Kamo
Interhelical interactions between D92 and C218 in the cytoplasmic domain regulate proton uptake upon N-decay in the proton transport of Acetabularia rhodopsin II
Journal: Journal of Photochemistry & Photobiology, B: Biology,183, 35-45 (2018)

180.Y. Nakajima, T. Tsukamoto, Y. Kumagai, Y. Ogura, T. Hayashi, J. Song, T. Kikukawa, M. Demura, K. Kogure, Y. Sudo, S. Yoshizawa
Presence of a Haloarchaeal Halorhodopsin-Like Cl- Pump in Marine Bacteria
Microbes Environ. 2018 Mar 16. doi: 10.1264/jsme2.ME17197. 

179. Yukiko Horie, Hideyuki Nemoto*, Jin Fujita, Shigeo Ikegawa, Yasuhiro Kumaki, Yuki Ohnishi, Hiroyuki Kumeta, Makoto Demura, Tomoyasu Aizawa 
Studies on the quality control of fermented brown rice and rice bran with Aspergillus oryzae by metabolome analysis using 1H-NMR
Nippon Shokuhin Kagaku Kogaku Kaishi, in press.
堀江裕紀子, 根本英幸*, 藤田仁, 池川繁男, 熊木康裕,　大西裕季, 久米田博之, 出村誠, 相沢智康
1H-NMRメタボローム解析を用いた玄米米糠麹(FBRA)の品質評価に関する基礎的検討
日本食品科学工学会誌,in press (2017)

178. Md. Ruhul Kuddus, Megumi Yamano, Farhana Rumi, Takashi Kikukawa, Makoto Demura, Tomoyasu Aizawa
Enhanced expression of cysteine-rich antimicrobial peptide snakin-1 in Escherichia coli using an aggregation-prone protein coexpression system 
Biotechnology progress , 2017 May 27. doi: 10.1002/btpr.2508.

177. K. Kajimoto, T. Kikukawa, H. Nakashima, H. Yamaryo, Y. Saito, T. Fujisawa, M. Demura, M. Unno
Transient Resonance Raman Spectroscopy of a Light-Driven Sodium-Ion-Pump Rhodopsin from Indibacter alkaliphilus
Journal of Physical Chemistry B, 121, 4431-4437 (2017)

176. J. Tamogami, T. Kikukawa, T. Nara, M. Demura, T. Kimura-Someya, M. Shirouzu, S. Yokoyama, S. Miyauchi, K. Shimono, N. Kamo
Existence of two O-like intermediates in the photocycle of Acetabularia rhodopsin II, a light-driven proton pump from a marine alga
Biophysics and Physicobiology, 14, 49-55 (2017)

175. T. Tsukamoto, S. Yoshizawa, T. Kikukawa, M. Demura, Y. Sudo
Implications for the Light-Driven Chloride Ion Transport Mechanism of Nonlabens marinus Rhodopsin 3 by Its Photochemical Characteristics
Journal of Physical Chemistry B, 121, 2017-2038 (2017)

174. S. Nakamura, T. Kikukawa, J. Tamogami, M. Kamiya, T. Aizawa, Martin W Hahn, K. Ihara, N. Kamo, M. Demura
Photochemical characterization of actinorhodopsin and its functional existence in the natural host 
Biochim. Biophys. Acta - Bioenergetics, 1857, 1900-1908 (2016)

173. M. Baek, M. Kamiya, T. Kushibiki, T. Nakazumi, S. Tomisawa, C. Abe, Y. Kumaki, T. Kikukawa, M. Demura, K. Kawano, Keiichi, T. Aizawa
Lipopolysaccharide-bound structure of the antimicrobial peptide cecropin P1 determined by nuclear magnetic resonance spectroscopy
Journal of Peptide Science, 22, 214-221 (2016)

172. Md. Ruhul Kuddus, Farhana Rumi, M. Tsutsumi, R. Takahashi, M. Yamano, M. Kamiya, T. Kikukawa, M. Demura, T. Aizawa
Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris
Protein Expression and Purification, 122, 15-22 (2016)

171. J. Tamogami, K. Sato, S. Kurokawa, T. Yamada, T. Nara, M. Demura, S. Miyauchi, T. Kikukawa, E. Muneyuki, N. Kamo
Formation of M-Like Intermediates in Proteorhodopsin in Alkali Solutions (pH >8.5) Where the Proton Release Occurs First 
in Contrast to the Sequence at Lower pH
Biochemistry, 55, 1036-1048 (2016)

170. H. Tamaki, A. Egawa, K. Kido, T. Kameda, M. Kamiya, T. Kikukawa, T. Aizawa, T. Fujiwara, M. Demura
Structure determination of uniformly 13C, 15N labeled protein using qualitative distance restraints 
from MAS solid-state 13C-NMR observed paramagnetic relaxation enhancement
Journal of Biomolecular NMR, 64,　87-101　(2016).

169. M. Tsutsumi, H.Muto, S. Myoba, M. Kimoto, A. Kitamura, M. Kamiya, T. Kikukawa, S. Takiya, M. Demura, K. Kawano, M. Kinjo, T. Aizawa
In vivo fluorescence-correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor
FEBS Open Bio, First published: 27 January 2016.

168. T. Hasemi, T. Kikukawa, N. Kamo, M. Demura
Characterization of a Cyanobacterial Chloride-Pumping Rhodopsin and Its Conversion into a Proton Pump
J. Biol. Chem., 291, 355-362 (2016)

167. T. Ose, A. Oikawa, Y. Nakamura, K. Maenaka, Y. Higuchi, Y. Satoh, Shiho Fujiwara, M. Demura, T. Sone, M. Kamiya, 
Solution structure of an avirulence protein, AVR-Pia, from Magnaporthe oryzae
Journal of Biomolecular NMR, 63, 229-235 (2015)

166. M. Furuse, J. Tamogami, T. Hosaka, T. Kikukawa, N. Shinya, M. Hato, N. Ohsawa, S. Y. Kim, K.-H. Jung, M. Demura, 
S. Miyauchi, N. Kamo, K. Shimono, T. Kimura-Someya, S. Yokoyama, M. Shirouzu
Structural Basis for Slow Photocycle and Late Proton Release in Acetabularia Rhodopsin I from the Marine Plant, 
Acetabularia acetabulum, and membrane potential-induced modulation of the photocycle
Acta Crystallographica Section D, 71, 2203-2216 (2015)

165. T. Kikukawa, C. Kusakabe, A. Kokubo, T. Tsukamoto, M. Kamiya, T. Aizawa, K. Ihara, N. Kamo, M. Demura
Probing the Cl--pumping photocycle of pharaonis halorhodopsin:  Examinations with bacterioruberin, an intrinsic dye, 
and membrane potential-induced modulation of the photo-cycle
Biochim. Biophys. Acta - Bioenergetics, 1847, 748-758 (2015).

164. S. Tomisawa, Y. Sato, M. Kamiya, Y. Kumaki, T. Kikukawa, K. Kawano, M. Demura, K. Nakamura, T. Ayabe, T. Aizawa
Efficient production of a correctly folded mouse a-defensin, cryptdin-4, by refolding during inclusion body solubilization
Protein Expression and Purification, 112, 21-28 (2015).

163. J. Tamogami, K. Iwano, A. Matsuyama, T. Kikukawa, M. Demura, T. Nara, N. Kamo
The effects of chloride ion binding on the photochemical properties of sensory rhodopsin II from Natronomonas pharaonis 
Journal of Photochemistry and Photobiology B: Biology, 141, 192-201 (2014).

162. T. Tsukamoto, M. Demura, Y. Sudo
Irreversible Trimer to Monomer Transition of Thermophilic Rhodopsin upon Thermal Stimulation
Journal of Physical Chemistry B, 118, 12383-12394 (2014).

161. T. Kushibiki, M. Kamiya, T. Aizawa, Y. Kumaki, T. Kikukawa, M. Mizuguchi, M. Demura, S.-I. Kawabata, K. Kawano 
Interaction between tachyplesin I, an antimicrobial peptide derived from horseshoe crab, and lipopolysaccharide.
Biochim. Biophys. Acta - Proteins and Proteomics, 1844, 527-534 (2014).

160. K. Shibasaki, H. Shigemura, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura 
Role of Thr218 in the Light-Driven Anion Pump Halorhodopsin from Natronomonas pharaonis.
Biochemistry, 52, 9257-9268 (2013).

159. T. Asakura, Y. Suzuki, A. Nagano, D. Knight, M. Kamiya, M. Demura
Synthesis and Characterization of Water-soluble Silk Peptides and Recombinant Silk Protein containing Polyalanine, 
the Integrin Binding Site and Two Glutamic Acids at Each Terminal Site as a Possible Candidate for Use in Bone Repair Materials
Biomacromolecules, 14, 3731-3741 (2013).

158. T. Tsukamoto, X. Li, H. Morita, T. Minowa, T. Aizawa, N. Hanagata, M. Demura
Role of S-palmitoylation on IFITM5 for the interaction with FKBP11 in osteoblast cells
PLoS One, 8, e75831 (2013).

157. S. Tomisawa, E. Hojo, Y. Umetsu, S. Ohki, Y. Kato, M. Miyazawa, M. Mizuguchi, M. Kamiya, Y. Kumaki, T. Kikukawa, K. Kawano, M. Demura, T. Aizawa
Overexpression of an antimicrobial peptide derived from C. elegans using an aggregation-prone protein coexpression system
AMB Express, 3, 45 (2013).

156. S. Tomisawa, C. Abe, M. Kamiya, T. Kikukawa, M. Demura, K. Kawano, T. Aizawa
A new approach to detect small peptides clearly and sensitively by Western blotting using a vacuum-assisted detection method,
Biophysics, 9, 79-83, 2013.

155. T. Nakamura, T. Aizawa, R. Kariya, S. Okada, M. Demura, K. Kawano, K. Makabe, K. Kuwajima
Molecular mechanisms of the cytotoxicity of human alpha-lactalbumin made lethal to tumor cells (HAMLET) and 
other protein-oleic acid complexes
J. Biol. Chem., 288, 14408-14416, 2013.

154. S. Hayashi, J. Tamogami, T. Kikukawa, H. Okamoto, K. Shimono, S. Miyauchi, M. Demura, T. Nara, N. Kamo
Thermodynamic Parameters of Anion Binding to Halorhodopsin from Natronomonas pharaonis by Isothermal Titration Calorimetry
Biophysical Chemistry, 172, 61-67 (2013).

153. T. Tsukamoto,T. Kikukawa, T. Kurata, K.H. Jung, N.Kamo, M. Demura
Salt Bridge in the Conserved His-Asp Cluster in Gloeobacter Rhodopsin Contributes to Trimer Formation
FEBS Lett., 587, 322-327 (2013).

152. 原田尚樹, 柏崎晴彦, 赤澤敏之, 村田勝, 相沢智康, 出村誠, 田中順三, 飯塚正, 井上農夫男
ラット頭頂骨骨膜下におけるrhBMP-2添加多孔性キトサン/ハイドロキシアパタイト複合体による骨形成
北海道歯誌, 32, 166-176 (2012).

151. J. Tamogami, T. Kikukawa, T. Nara, K. Shimono, M. Demura, N. Kamo, Naoki
The Photo-induced Proton Release in Proteorhodopsin at Low pH: The Possibility of the pKa Decrease of Asp227
Biochemistry, 51, 9290-9301 (2012).

150. L. Reissig, T. Iwata, T. Kikukawa, M. Demura, N. Kamo, H. Kandori, Y. Sudo
Influence of halide binding on the hydrogen bonding network in the active site of Salinibacter sensory rhodopsin I
Biochemistry, 51, 8802-8813 (2012)..

149. Y. Furutani, K. Fujiwara, T. Kimura, T. Kikukawa, M. Demura, H. Kandori
Dynamics of Dangling Bonds of Water Molecules in pharaonis Halorhodopsin during Chloride Ion Transportation
J.Phys.Chem.Lett., 3, 2964-2969 (2012).

148. M. Mizuguchi, M. Takeuchi, Y. Nabeshima, S. Ohki, T. Kouno, T. Aizawa, M. Demura, K. Kawano, K. Yutani
Structural characterization of a trapped folding intermediate of pyrrolidone carboxyl peptidase from a hyperthermophile
Biochemistry, 51, 6089-6096 (2012).

147. K. Muroda, K. Nakashima, M. Shibata, M. Demura, H. Kandori 
Protein-Bound Water as the Determinant of Asymmetric Functional Conversion between Light-Driven Proton and Chloride Pumps
Biochemistry, 51, 4677-4684 (2012).

146. T. Tsukamoto, T. Sasaki, K. J. Fujimoto, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
Homo-trimer Formation and Dissociation of pharaonis Halorhodopsin in Detergent System
Biophysical J., 102, 2906-2915 (2012).

145. J. Tamogami, T. Kikukawa, Y. Ikeda, M. Demura, T. Nara, N. Kamo
Photo-induced Bleaching of Sensory Rhodopsin II (Phoborhodopsin) from Halobacterium salinarum by Hydroxylamine: 
Identification of the Responsible Intermediates
Journal of Photochemistry and Photobiology B: Biology, 106, 87-94 (2012).

144. T. Asakura, H. Nishi, A. Nagano, A. Yoshida, Y. Nakazawa, M. Kamiya, M. Demura
NMR analysis of the fibronectin cell-adhesive sequence, Arg-Gly-Asp, in a recombinant silk-like protein and a model peptide
Biomacromolecules, 12, 3910-3916 (2011).

143. Y. Yamashita, T. Kikukawa, T. Tsukamoto, M. Kamiya, T. Aizawa, K. Kawano, S. Miyauchi, N. Kamo, M. Demura,
Expression of salinarum halorhodopsin in Escherichia coli cells: solubilization in the presence of retinal yields the natural state
Biochim. Biophys. Acta, 1808, 2905-2912, (2011).

142. G. Dai, Y. Zhang, J. Tamogami, M. Demura, N. Kamo, H. Kandori, T. Iwasa
An amino acid residue (S201) in the retinal binding pocket regulates the photoreaction pathway of phoborhodopsin
Biochemistry, 50, 7177-7183 (2011).

141. T. Sasaki, M. Demura, N. Kato, Y. Mukai
Sensitive detection of protein-lipid interaction change on bacteriorhodopsin using Dodecyl-β-D-maltoside
Biochemistry,50, 2283-2290 (2011). 

140. T. Kouno, N. Watanabe, N. Sakai, T. Nakamura, Y. Nabeshima, M. Morita, M. Mizuguchi, T. Aizawa, M. Demura, T. Imanaka, I. Tanaka, K. Kawano
The structure of Physarum polycephalum hemagglutinin I suggests a minimal carbohydrate recognition domain of legume lectin fold
J. Mol. Biol., 405, 560-569 (2011).

139.K. Higa, S. Shimmura, N. Takeshima, F. Moro, T. Kawakita, M. Kawashima, M. Demura, J. Shimazaki, T. Asakura, K. Tsubota
Porous silk fibroin film as a transparent carrier for cultivated corneal epithelial sheets
Journal of Biomaterials Science: Polymer Edition, 22, 2261-2276 (2011).

138. W. Wang, S. Itoh, T. Aizawa, K. Sakai, T. Ohkuma, M. Demura
Development of an injectable chitosan/marine collagen composite gel
Biomedical Materials, 5 065009 (2010).

137. Y. Nonaka, H. Muto, T. Aizawa, E. Okabe, S. Myoba, T. Yokoyama, S. Saito, F. Tatami, Y. Kumaki, 
M. Kamiya, T. Kikukawa, M. Mizuguchi, S. Takiya, M. Kinjo, M. Demura, K. Kawano
STPR, A 23-amino-acid tandem repeat domain, found in the human function-unknown protein ZNF821
Biochemistry, 49, 8367-8375 (2010).

136. M. Takahashi, M. Mizuguchi, H. Shinoda, T. Aizawa, M.Demura, H. Okazawa, K. Kawano
Polyglutamine tract-binding protein-1 binds to U5-15kD via a continuous 23-residue segment of the C-terminal domain
Biochim. Biophys. Acta - Proteins and Proteomics, 1804, 1500-1507 (2010).

135. M. Kamiya, K. Oyauchi, Y. Sato, T. Yokoyama, M. Wang, T. Aizawa, Y. Kumaki, M. Mizuguchi, 
K. Imai, M. Demura, K. Suzuki, K. Kawano
Structure-activity relationship of a novel pentapeptide with cancer cell growth-inhibitory activity. 
J.Peptide Sci., 16, 242-248 (2010).

134.　J. Tamogami, T. Kikukawa, Y. Ikeda, A. Takemura, M. Demura,，N. Kamo
The photochemical reaction cycle and photo-induced proton transfer of sensory rhodopsin II (phoborhodopsin) 
from Halobacterium salinarum
Biophys. J., 98, 1353-1363 (2010). 

133. T. Sasaki, T. Aizawa, M. Kamiya, T. Kikukawa, K. Kawano, N. Kamo, M. Demura
Effect of chloride binding on the thermal trimer-monomer conversion of halorhodopsin in the solubilized system
Biochem., 48, 12089-12095 (2009).

132. M. Sonoyama, T. Kikukwa, Y. Yokoyama, M. Demura, N. Kamo, S. Mitaku
Effect of Molecular Assembly on Photocycle of Reconstituted Bacteriorhodopsin: 
Significant Blue Shift of the Late M Photointermediate in the Liquid Crystalline Phase
Chem. Lett.,　38, 1134-1135 (2009).

131. Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K.
C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity
J Biol Chem., 284, 29625-29634 (2009).

129.　H. Ishihara, G. Matsuo, T. Sasaki, Y. Saito, M. Demura, K. Tsujii
Electrophoresis of Dyes and Proteins in Poly(Acrylamide) Gel Containing Immobilized Bilayer Membranes
Progress in Colloid and Polymer Science, 136, 143-148 (2009)

128.T. Kouno , M. Mizuguchi , T. Aizawa , H. Shinoda , M. Demura , S.-i. Kawabata and K. Kawano
A novel -defensin structure: big defensin changes its N-terminal structure to associate with the target membrane
Biochemistry, 48, 7629-7635 (2009).

127. K. Nakashima, T. Nakamura, S. Takeuchi, M. Shibata, M. Demura, T. Tahara, H. Kandori
Property of Anion Binding Site of pharaonis Halorhodopsin Studied by Ultrafast Pump-Probe Spectroscopy and 
Low-Temperature FTIR Spectroscopy
Journal of Physical Chemistry, 113, 8429-8434 (2009). 

126. S. Nakatogawa, Y. Oda, M. Kamiya, T. Kamijima, T. Aizawa, K. D. Clark, M. Demura, K. Kawano, M. R. Strand, Y. Hayakawa
A Novel Peptide Mediates Aggregation and Migration of Hemocytes From an Insect
Current Biology, 19, 779-785 (2009).

125. S. Takiya, S. Saito, T. Yokoyama, D. Matsumoto, T. Aizawa, M. Kamiya, M. Demura, and Keiichi Kawano
DNA-Binding Property of the Novel DNA-Binding Domain STPR in FMBP-1 of the Silkworm Bombyx mori
J. Biochem., 146, 103-111 (2009).

124. M. Takahashi, M. Mizuguchi, H. Shinoda, T. Aizawa, M. Demura, H. Okazawa,  K. Kawano
Polyglutamine tract binding protein-1 is an intrinsically unstructured protein
Biochimica et Biophysica Acta - Proteins and Proteomics, 1794, 936-943 (2009). 

123. Nonaka, Y.; Akieda, D.; Aizawa, T.; Watanabe, N.; Kamiya, M.; Kumaki, Y.; Mizuguchi, M.; Kikukawa, T.; Demura, M.; Kawano, K.
X-ray crystallography and structural stability of digestive lysozyme from cow stomach
FEBS J., 276, 2192-2200 (2009).

122. K. Inoue, M. Kubo, M. Demura, N. Kamo, M. Terazima
Reaction dynamics of halorhodopsin studied by time-resolved diffusion method
Biophys. J., 96, 3724-3734 (2009).

121. M. Kubo, T. Kikukawa, S. Miyauchi, A. Seki, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo, M. Demura
Role of Arg123 in light-driven anion pump mechanisms of pharaonis halorhodopsin
Photochem. Photobiol., 85, 547-555 (2009).

120. T. Kamijima, A. Ohmura; T. Sato, K. Akimoto M. Itabashi,　M. Mizuguchi, M. Kamiya, T. Kikukawa, 
T. Aizawa, M. Takahashi, K. Kawano, M. Demura
Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death
Biochem.Biophys.Res.Commun., 376, 211-214 (2008), Epub 2008 Sep 5.

119. Kouno, T.; Fujitani, N.; Mizuguchi, M.; Osaki, T.; Nishimura, S.-I.; Kawabata, S.-i.; Aizawa, T.; 
Demura, M.; Nitta, K.; Kawano, K., 
A Novel beta-defensin Structure: a Strategy of Big Defensin for a Resistance by Gram-positive Bacteria
Biochemistry, 47, 10611-10619 (2008).

118. T. Nakamura, S. Takeuchi, M. Shibata, M. Demura, H. Kandori, T. Tahara
Ultrafast Pump-Probe Study of the Primary Photoreaction Process in pharaonis Halorhodopsin: 
Halide-Ion Dependence and Isomerization Dynamics
Journal of Physical Chemistry B, 112, 12795-12800 (2008).

117. T. Sasaki, M. Kubo, T. Kikukawa, M. Kamiya, T. Aizawa, K. Kawano, N. Kamo and M. Demura
Halorhodopsin from Natronomonas pharaonis forms a trimer even in the presence of a detergent, 
dodecyl-β-D-maltoside.
Photochem. Photobiol., 85, 130-136, 2009.

116. Mizuguchi M, Hayashi A, Takeuchi M, Dobashi M, Mori Y, Shinoda H, Aizawa T, Demura M, Kawano K.
Unfolding and aggregation of transthyretin by the truncation of 50 N-terminal amino acids.
Proteins, 72, 261-269 (2008).

115. Wang W, Itoh S, Matsuda A, Aizawa T, Demura M, Ichinose S, Shinomiya K, Tanaka J.,
Enhanced nerve regeneration through a bilayered chitosan tube: The effect of introduction of 
glycine spacer into the CYIGSR sequence
J Biomed Mater Res A, 85A, 919-928 (2008)

114. S. Saito, T. Yokoyama, T. Aizawa, K. Kawaguchi, T. Yamaki, D. Matsumoto, T. Kamijima, M. Kamiya, Y. Kumaki, 
M. Mizuguchi, S. Takiya, M. Demura, K. Kawano,
Structural Properties of the DNA-Bound Form of a Novel Tandem Repeat DNA-Binding Domain, STPR
Proteins, 72, 414-426 (2008)

113. Y. Nonaka, T. Aizawa, D. Akieda, M. Yasui, M. Watanabe, N. Watanabe, I. Tanaka, M. Kamiya, M. Mizuguchi, 
M. Demura, K. Kawano,
Spontaneous asparaginyl deamidation of canine milk lysozyme under mild conditions
Proteins, 72, 313-322 (2008)

112. Kouno T, Mizuguchi M, Tanaka H, Yang P, Mori Y, Shinoda H, Unoki K, Aizawa T, 
Demura M, Suzuki K, Kawano K., The Structure of a Novel Insect Peptide Explains 
Its Ca(2+) Channel Blocking and Antifungal Activities,　
Biochemistry, 46, 13733-13741 (2007).

111. Fujitani N, Kouno T, Nakahara T, Takaya K, Osaki T, Kawabata S, Mizuguchi M, Aizawa T, 
Demura M, Nishimura S, Kawano K.
The solution structure of horseshoe crab antimicrobial peptide tachystatin B with an 
inhibitory cystine-knot motif" 
J Pept Sci. , 13, 269-279 (2007). 

110.  H. Nakatani, K. Maki,K. Saeki, T. Aizawa, M. Demura, K. Kawano, 
S. Tomoda, K. Kuwajima, Kunihiro
Equilibrium and Kinetics of the Folding and Unfolding of Canine Milk Lysozyme
Biochemistry, 46, 5238 - 5251 (2007).

109. S. Saito, T. Aizawa, K. Kawaguchi, T. Yamaki, D. Matsumoto, M. Kamiya, Y. Kumaki, 
M. Mizuguchi, S. Takiya, M. Demura, K. Kawano
Structural Approach to a Novel Tandem Repeat DNA-Binding Domain, STPR, by CD and NMR
Biochemistry, 46, 1703-1713 (2007).

108. A. Seki, S. Miyauchi, S. Hayashi, T. Kikukawa, M. Kubo, M. Demura, V. Ganapathy, N. Kamo
Heterologous expression of pharaonis halorhodopsin in Xenopus laevis oocytes and 
electrophysiological characterization of its light-driven Cl- pump activity
Biophys. J., 92, 2559-2569 (2007).

107. M. Shibata, Y. Saito, M. Demura, H. Kandori
Deprotonation of Glu234 during the photocycle of Natronomonas pharaonis halorhodopsin
Chemical Physics Letters, 432, 545-547 (2006).

106. Takeuchi, M., Mizuguchi, M., Kouno, T., Shinohara, Y., Aizawa, T., Demura, M., 
Mori, Y., Shinoda H. and Kawano, K.
Destabilization of Transthyretin by Pathogenic Mutations in the DE loop
PROTEINS: Structure, Function, and Bioinformatics, 66, 716-725 (2007).

105. S. Watanabe, M. Tada, T. Aizawa, M. Yoshida, T. Sugaya, M. Taguchi, T. Kouno, T. 
Nakamura, M. Mizuguchi, M. Demura, Y. Hayakawa,  K. Kawano
N-Terminal Mutational Analysis of the Interaction between Growth-Blocking Peptide (GBP) 
and Receptor of Insect Immune Cells
Protein & Peptide Lett., 13, 815-822 (2006).

104.C. Hasegawa, T. Kikukawa, S. Miyauchi, A. Seki,Y. Sudo, M. Kubo, M. Demura, N. Kamo
Interaction of the Halobacterial Transducer to a Halorhodopsin Mutant Engineered so as to 
Bind the Transducer: Cl- Circulation within the Extracellular Channel
Photochem. Photobiol., 83, 293-302 (2007).

103. M. Yasui, T. Miyahara, T. Aizawa, M. Demura, K. Nitta
Differential Scanning Calorimetry of a Metalloprotein under Controlled Metal-Ion Activity
Protein J., 25, 475-482 (2006).

102. K. Arita, M. Akiyama, T. Aizawa, Y. Umetsu, I. Segawa, M. Goto, D. Sawamura, M. Demura, K. 
Kawano, H. Shimizu
A Novel N14Y Mutation in Connexin26 in KID Syndrome ? Analyses of Altered Gap Junctional Communication 
and Molecular Structure of N-Terminus of Mutated Connexin26
Am. J. Pathology, 169, 416-423 (2006).

101. N. Muneda, M. Shibata, M. Demura, H. Kandori
Internal Water Molecules of the Proton-Pumping Halorhodopsin in the Presence of Azide
J. Am. Chem. Soc., 128, 6294-6295 (2006).

100. M. Tada, Y. Shinohara, I. Kato, K. Hiraga, T. Aizawa, M. Demura,Y. Mori, H. Shinoda,
 M. Mizuguchi, K. Kawano
Preparation and Observation of Fresh-frozen Sections of the Green Fluorescent Protein 
Transgenic Mouse Head
Acta Histochemica et Cytochemica, 39, 31-34 (2006).

99.  J.-H. Yeo, K.-G. Lee, H.-Y. Kweon, S.-O. Woo, S.-M. Han, S.-S. Kim, M. Demura
Fractionation of Silk Fibroin Hydrolysate and Its Protective Function of Hydrogen Peroxide Toxicity
J. Appl. Polym. Sci., 102, 772-776 (2006).

98.　M. Kubo, M. Sato, T. Aizawa, C. Kojima, N. Kamo, M. Mizuguchi, K. Kawano, M. Demura
Disassembling and bleaching of chloride-free pharaonis halorhodopsin by octyl-beta-glucoside
Biochemistry, 44, 12923-12931 (2005).

97. M. Shibata , N. Muneda , T. Sasaki , K. Shimono, N. Kamo, M. Demura, H. Kandori 
Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule 
in the Chloride Pump of Natronobacterium pharaonis
Biochemistry, 44, 12279-12286 (2005).

96. T. Sasaki, M. Sonoyama, M. Demura, S. Mitaku, 
Photobleaching of bacteriorhodopsin solubilized with Triton X-100, 
Photochemistry and Photobiology, 81, 1131-1137 (2005).

95.　M. Mizuguchi, A. Matsuura, Y. Nabeshima, K. Masaki, M. Watanabe, T. Aizawa, 
M. Demura, K. Nitta, Y. Mori, H. Shinoda, K. Kawano
Effects of the Stabilization of the Molten Globule State on the Folding Mechanism of 
α-Lactalbumin: A Study of a Chimera of Bovine and Human α-Lactalbumin,  
Proteins, 61, 356-365 (2005).

94.　M. Sato, M. Kubo, T. Aizawa, N. Kamo, T. Kikukawa, K. Nitta, and M. Demura
Role of Putative Anion-Binding Sites in Cytoplasmic and Extracellular Channels 
of Natronomonas pharaonis Halorhodopsin, Biochemistry, 44, 4775-4784 (2005).

93. M. Demura, T. Yoshida, T. Hirokawa, Y. Kumaki, T. Aizawa, K. Nitta, I. Bitter, 
K. Toth, Interaction of dopamine and acetylcholine with an amphiphilic resorcinarene  
receptor in aqueous micelle system, Bioorganic & Medicinal Chemistry Letters, 
15, 1367-1370 (2005)

92. M. Yoshida, T. Aizawa, T. Nakamura, K. Shitara, Y. Hayakawa, K. Matsubara, 
K. Miura, T. Kouno, K. D. Clark, M. R. Strand, M. Mizuguchi, M. Demura, K. Nitta, 
K. Kawano, The Gly-Gly Linker Region of the Insect Cytokine Growth Blocking Peptide 
(GBP) is Essential for Activity, J. Biol. Chem., 279, 51331-51337 (2004).

91. M. Watanabe, T. Aizawa, M. Demura, and K. Nitta, Effect of Hydrostatic Pressure on 
Conformational Changes of Canine Milk Lysozyme between the Native, Molten Globule, and 
Unfolded States, Biochim. Biophys. Acta, 1702, 129-136 (2004).

90. M. Shibata, N. Muneda, K. Ihara, T. Sasaki, M. Demura, and H. Kandori,
Internal Water Molecules of Light-Driven Chloride Pump Proteins, Chem. Phys. Lett., 392, 
330-333 (2004).

89. M. Watanabe, Y. Kobashigawa, T. Aizawa, M. Demura, and K. Nitta, A Non-Native 
alpha-Helix Is Formed in the beta-Sheet Region of the Molten Globule State of 
Canine Milk Lysozyme, Protein Journal, 23, 335-342 (2004).

88. M. Watanabe, Y. Kobashigawa, T. Aizawa, M. Demura, K. Nitta, 
Volumetric behavior of the molten globule state of canine milk lysozyme, 
Protein Pept. Lett.,11, 325-330 (2004). 

87. M. Sato, T. Kikukawa, T. Araiso, H. Okita, K. Shimono, N. Kamo, M. Demura, and K. Nitta, 
Role of Ser130 and Thr126 in the Chloride Binding and Photocycle of pharaonis Halorhodopsin, 
J. Biochem.,134,　151-158 (2003). 

86. M. Sato, T. Kikukawa, T. Araiso, H. Okita, K. Shimono, N. Kamo, M. Demura, and K. Nitta, 
Ser-130 of Natronobacterium pharaonis Halorhodopsin Is Important for the Chloride Binding, 
Biophys. Chem., 104,　209-216 (2003).

85. N. Fujitani, M. Kanagawa, T. Aizawa, T. Ohkubo, S. Kaya, M. Demura, K. Kawano, K. Taniguchi, 
K. Nitta, Structure Determination and Conformational Change Induced by Tyrosine Phosphorylation 
of the N-terminal Domain of the a-Chain of Pig Gastric H+/K+-ATPase, Biochem. Biophys. Res. Commun., 
300, 223-229 (2003)

84. A. Matsuura, M. Yao, T. Aizawa, N. Koganesawa, K. Masaki, M. Miyazawa, M. Demura, I. Tanaka, 
 K. Kawano, K. Nitta, Structural Analysis of an Insect Lysozyme Exhibiting Catalytic Efficiency 
 at Low Temperatures, Biochemistry, 41, 12086-12092 (2002).

83. M. Tada, Y. Kobashigawa, M. Mizuguchi, K. Miura, T. Kouno, Y. Kumaki, M. Demura, K. Nitta, 
K. Kawano, Stabilization of protein by replacement of a fluctuating loop: structural analysis 
of a chimera of bovine alpha-lactalbumin and equine lysozyme., Biochemistry, 41, 13807-13813 (2002).

82. T. Suetake, T. Aizawa, N. Koganesawa, T. Osaki, Y. Kobashigawa, M. Demura, S.Kawabata, K.Kawano, 
S.Tsuda, and K.Nitta, Production and Characterization of Recombinant Tachycitin, the Chitin-Binding 
Protein, Protein Engineering, 15, 763-769(2002). 

81. K. Sasahara, M. Demura, and K. Nitta, Equilibrium and Kinetic Folding of Hen Egg-white 
Lysozyme under Acidic Conditions, Proteins, 49, 472-82 (2002).

80. N. Fujitani, S. Kawabata, T. Osaki, Y. Kumaki, M. Demura. K. Nitta and K. Kawano, 
Structure of the antimicrobial peptide Tachystatin A, J. Biol. Chem., 277, 23651-23657 (2002).

79. M.Mizuguchi, Y. Kobashigawa, Y. Kumaki, M. Demura, K. Kawano and K. Nitta, 
Effects of a Helix Stabilization on the Folding Mechanism of alpha-lactalbumin, 
Proteins, 49, 95-103 (2002).

78. N. Koganesawa, T.Aizawa, H.Shimojo, K.Miura, A.Ohnishi, M. Demura, Y.Hayakawa, K.Nitta, K.Kawano, 
Expression and purification of small cytokine growth-blocking peptide (GBP) from armyworm 
Pseudaletia separata by the optimized fermentation method using methylotrophic yeast Pichia 
pastoris, Protein Expression and Purification, 25, 416-425 (2002).

77. T. Miyazaki, T. Kaneko, J.P. Gong, Y. Osada, M. Demura, M. Suzuki, Water-Induced 
Crystallization of Hydrogels, Langmuir, 18, 965-967, (2002).

76. M. Sato, T. Kanamori, N. Kamo, M. Demura and K. Nitta, Stopped-Flow Analysis on Anion Binding 
to Blue-form Halorhodopsin from Natronobacterium pharaonis: Comparison with Anion-Uptake Process 
during Photocycle", Biochemistry, 41, 2452-2458 (2002).

75. M. Demura, M. Noda, T. Kajimoto, T. Uchiyama, K. Umemoto, C.-H. Wong, T. Asakura, Solution 
Structure of Sialyl Lewis X mimic Studied by Two-dimensional NMR, J.Mol.Struc., 602, 215-222
(2002).

74. T. Koshiba, Y. Kobashigawa, M. Demura, and K. Nitta, Energetics of three-state unfolding 
of a protein: canine milk lysozyme, Protein Eng. 14, 967-974 (2001).

73. Aizawa T, Hayakawa Y, Ohnishi A, Fujitani N, Clark KD, Strand MR, Miura K, Koganesawa N, 
Kumaki Y, Demura M, Nitta K, Kawano K., Structure and activity of the insect cytokine growth-
blocking peptide. Essential regions for mitogenic and hemocyte-stimulating activities are 
separate, J. Biol. Chem., 276, 31813-31818 (2001).

72. Y. Kurokawa, N. Koganesawa, Y. Kobashigawa, T.Koshiba, M. Demura, and K. Nitta, Oxidative 
Folding of Human Lysozyme: the Effects of the Loss of Two Disulfide Bonds and the 
Introduction of a Calcium-Binding Site, J. Protein Chem.,20, 293-303 (2001).

71. T. Niidome, H. Murakami,  M. Kawazoe, T. Hatakeyama, Y. Kobashigawa, M. Matsushita, Y. 
Kumaki, M. Demura, K. Nitta, and H. Aoyagi, Carbohydrate Recognition of Gramicidin S Analogs 
in Aqueous Medium, Bioorganic Medicinal Chemistry Letters, 11, 1893-1896 (2001).

70. Y. Kobashigawa, K. Miura, M. Demura, N. Nemoto, T. Koshiba, K. Nitta, and S. Tsuda, 
Assignment of 1H, 13C, and 15N Resonances of Canine Milk Lysozyme, Journal of Biomolecular 
NMR, 19, 387-388 (2001).

69. Y. Hori, M. Demura, M. Iwadate, A. S. Ulrich, T. Niidome, H. Aoyagi, and T. Asakura, Interaction 
of Mastoparan with Membranes Studied by 1H-NMR in Detergent Micelles and by Solid State 
2H-NMR and 15N-NMR in Oriented Lipid Bilayers, Eur. J. Biochem., 267, 302-309 (2001).

68. J.D.van Beek, L. Beaulieu, H. Schafer, M. Demura, T. Asakura and B.H. Meier, Solid-state NMR 
determination of the secondary structure of Samia cynthia ricini silk, Nature, 405, 1077-1079 
(2000).

67. Y. Kobashigawa, M. Demura, T. Koshiba, Y. Kumaki, K. Kuwajima, K. Nitta, Hydrogen Exchange 
Study of Canine Milk Lysozyme: Stabilization Mechanism of the Molten Globule, Proteins, 40, 
579-589 (2000).

66. K. Sasahara, M. Demura, K. Nitta, Partially Unfolded Equilibrium State of Hen Lysozyme Studied 
by Circular Dichroism Spectroscopy, Biochemistry, 39, 475-6482 (2000).

65. M. Mizuguchi, K. Masaki, M. Demura and K. Nitta, Local and Long-range Interactions in the 
Molten Globule State: a study of chimeric proteins of bovine and human alpha-lactalbumin. J. 
Mol. Biol., 298, 985-995 (2000). 

64. M. Osanai, M. Okudaira, J. Naito, M. Demura, T. Asakura, Biosynthesis of L-alanine, a major 
amino acid of fibroin in Samia Cynthia ricini, Insect Biochemistry and Molecular Biology, 30, 
225-232 (2000).

63. T. Koshiba, M. Yao, Y. Kobashigawa, M. Demura, A. Nakagawa, I. Tanaka, K. Kuwajima and K. 
Nitta, Structure and thermodynamics of the extraordinarily stable molten globule state of canine 
milk lysozyme, Biochem., 39, 3248-3257 (2000).

62. T. Ito, Y. Maruhashi, M. Demura and T. Asakura, Carbon-13 solid state NMR study on uniaxially 
oriented poly(L-lactic) films, Polymer 41, 859-866 (2000).

61. A. Higuchi, S. Uchiyama, M. Demura, T. Asakura, C.-S. Cho, T. Akaike, H. Takarada and M. Hara, 
Enhanced CEA production associated with aspirin in a culture of CW-2 cells on some polymeric 
films" Cytotechnology, 31, 233-242 (1999).

60. T. Asakura and M. Demura, Structure and Dynamics of Silk Fibroin Studied with 13C, 15N and 
2H Solid State NMR, Macromol., Macromolecular Symposia, 143, 1-10 (1999). 

59. Y. Hori, M. Demura, T. Niidome, H. Aoyagi, T. Asakura,  Orientational behavior of phospholipid 
membranes with mastoparan studied by 31P solid state NMR, FEBS Lett. 455,228-232 (1999).

58. T. Asakura, M. Iwadate, M. Demura and M. P. Williamson, Structural analysis of silk with 13C 
NMR chemical shift contour plots, International Journal of Biological Macromolecules, 24, 167-
171 (1999).

57. T. Asakura, Y. Yamazaki,  W.-S. Koo  and M. Demura, Determination of the Mutual Orientation of 
the 15N and 13C NMR Chemical Shift Tensors of 13C-15N Double Labeled Model Peptides for 
Silk Fibroin from the Dipolar-Coupled Powder Patterns, J. Molecular Structure, 446, 179-190 
(1998).

56. M. Demura, M. Minami, T. Asakura, and T. A. Cross, Structure of Bombyx mori Silk Fibroin based 
on Solid State NMR Orientational Constraints and Fiber Diffraction Unit Cell Parameters, J. Am. 
Chem. Soc., 120, 1300-1308 (1998).

55. M. Demura, Y. Yamazaki, T. Asakura and K. Ogawa, Structure of Uniaxially Aligned 13C Labeled 
Silk Fibroin Fibers with Solid State 13C-NMR, J. Molecular Structure, 441,155-163 (1998).

54. T. Asakura, M. Minami, R. Shimada, M. Demura, M. Osanai, T. Fujito, M. Imanari, and A. S. Ulrich. 
1997. 2H Labeling of Bombyx mori Silk Fibroin and The Structural Characterization Using Solid 
State 2H NMR. Macromolecules, 30, 2429-2435 (1997).

53. T. Asakura, M. Demura, T. Date, M. Miyashita, K. Ogawa, and M. P. Williamson. NMR Study of 
Silk I Structure of Bombyx mori  Silk Fibroin with 15N and 13C NMR Chemical Shift Contour 
Plots, Biopolymers, 41, 193-203 (1997).

52. T. Asakura, M. Iwadate, T. Date, M. Demura and K. Nokihara, Refinement of the Solution 
Structure of Neurokinin A (4-10) on the Basis of 1H NMR Chemical Shift Calculations, Peptide 
Chem. 345-348 (1996).

51. T. Asakura, A. Aoki, T. Date, M. Demura, High-Resolution Solid State 13C NMR Spectroscopy of 
Syndiotactic Polypropylene, Polymer J., 28, 24-29 (1996).

50. T. Asakura, T. Konakazawa, M. Demura, T. Ito, and Y. Maruhashi. Structural analysis of uniaxially 
oriented 13C-labelled poly(ethylene terephthalate) films studied with solid-state 13C nuclear 
magnetic resonance spectroscopy. Polymer, 37, 1965-1973 (1996). 

49. T. Asakura, K. Taoka, M. Demura and M. P. Williamson, The Relationship between Amide Proton 
Chemical Shifts and Secondary Structure in Proteins, J. Biomol. NMR, 6, 227-236 (1995).

48. M. Demura and T. Asakura, Structural Characterization of Samia cynthia ricini Silk Fibroin as 
Biomaterial, Int. J. Wild Silkmoth & Silk, 1, 139-141 (1994).

47. T. Asakura, A. Aoki, M. Demura, J. M. Joers, R. C. Rosanske, and T. Gullion, Structure of Bombyx 
mori Silk Fibroin Studied by REDOR NMR Spectroscopy, Polymer J., 26, 1405 - 1408 (1994).

46. 北村愛夫、陳開利、柴本秋男、出村誠、有機溶媒処理絹の凝集構造変化、日本蚕糸学雑誌, 63, 415- 419 (1994).

45. 呂奏洪、出村誠、小中沢岳仁、朝倉哲郎、伊藤卓郎、藤戸輝昭、今成司、固体15N NMRによる ポリアミド系繊維の構造解析、
高分子論文集、51, 47-51 (1994).

44. J.-H. Yeo, M. Demura, T. Asakura, T. Fujito, M. Imanari, L. K. Nicholson and T. A. Cross, 
Structural analysis of highly oriented poly (P-phenylene-ferephthalamide ) by 15N solid-state 
nuclear magnetic resonance, Solid State Nuclear Magnetic Resonance 3, 209-218 (1994).

43. M. Minami, R. Takatsu, M. Demura and T. Asakura, A Study on the Hydration of Bombyx mori 
Silk Fibroin by Nuclear Magnetic Resonance Spectroscopy, Sen-i Gakkaishi, 50, 498-504 (1994).

42. T. Asakura, M. Demura, Y. Hiraishi, K. Ogawa, and A. Uyama, Determination of the Structure of 
[1-13C]Glycine-[15N]Alanine Double Labeled Bombyx mori Silk Fibroin Fibers Using Solid State 
15N NMR, Chem. Lett., 2249-2252 (1994).

41. A. Ono, S. Miyauchi, M. Demura, T. Asakura and N. Kamo, Activation Energy for Permeation of 
Phoshonium Cations Through Phospholipid Bilayer Membrane, Biochemistry, 33,  4312- 4318 
(1994).

40. T. Asakura, R. Sakaguchi, M. Demura, K. Ogawa, M. Osanai,  In vitro Production of Bombyx mori 
Silk Fibroin by Organ-Culture of the Posterior Silk Glands, and the Isotope Labeling and 
Fluorination of the Silk Fibroin, Biotechnol.Bioeng., 41, 245-252 (1993).

39. L. K. Nicholson, T. Asakura, M. Demura, T. A. Cross,  A Method for Studying the Structure of 
Uniaxially Aligned Biopolymers Using Solid State 15N NMR: Application to Bombyx mori Silk 
Fibroin Fibers, Biopolymers, 33, 847-861 (1993).

38. T. Asakura, J.-H. Yeo, M. Demura, T. Itoh, T. Fujito, M. Imanari, L. K. Nicholson, T. A. Cross, 
Structural Analysis of Uniaxially Aligned Polymers Using Solid State 15N NMR, Macromolecules, 
26, 6660-6663 (1993). 

37. 北村愛夫、陳開利、柴本秋男、出村誠、絹の有機溶媒中における伸長、収縮、日本蚕糸学会誌, 61, 215-220 (1992).

36. 出村誠、竹之下仁子、朝倉哲郎、酒井治利、栗岡聡、小松計一、金子正夫、 絹セリシン／絹フィブロインブレンド
コーティングによる酵素固定化不織布、日本蚕糸学雑誌、61, 66-72 (1992).

35. M. Demura, T. Takekawa, T. Asakura and A. Nishikawa, Characterization of Low Temperature 
Plasma-Treated Silk Fibroin Fabrics by ESCA and  the Use of the Fabrics to an Enzyme- 
Immobilized Support, Biomaterials, 13, 276-280 (1992).

34. T. Asakura, M. Kitaguchi, M. Demura, H. Sakai and K. Komatsu,  Immobilization of Glucose 
Oxidase on Non-Woven Fabrics with Bombyx mori Silk  Fibroin gel,  J. Appl. Polym. Sci., 46, 49 
- 53 (1992).

33. T. Asakura, M. Demura, Y. Watanabe, K. Sato, 1H pulsed NMR Study of Bombyx mori Silk 
Fibroin: Dynamics of Silk Fibroin and of Absorbed Water, J. Polym. Sci., Part B: Polym. Phys., 30, 
693 - 699 (1992). 

32. M. P. Williamson, T. Asakura, E. Nakamura and M. Demura, A method for the calculation of 
protein α-CH chemical shifts, J. Biomolecular NMR, 2, 83-89 (1992).

31. 朝倉哲郎、出村誠、中村英二、安藤勲、Basic Pancreatic Trypsin Inhibitorのα-CHおよびNH 1H　NMR化学シフトの理論計算、
高分子論文集、49, 281-287 (1992).  

30. T. Asakura, N. Nakayama, M. Demura, A. Asano, Carbon-13 Spectral NMR  Assignment of 
regioirregular Polypropylene Determined from Two-Dimensional  INADEQUATE Spectrum and 
the Chemical Shift Calculation, Macromolecules, 25, 4876-4881 (1992).

29. 朝倉哲郎、北口源啓、出村誠、酒井治利、金子正夫、栗岡聡、小松計一、不織布表面にコｰ ティン グされたスピンラベル
グルコースオキシダーゼならびにスピンラベル絹フィブロインのＥＳＲ解析, 日本蚕糸学雑誌、60, 466-474 (1991).

28. M. Demura and T. Asakura, Porous Membrane of Bombyx mori Silk Fibroin: Structure 
Characterization, Physical Properties and Application to Glucose Oxidase- Immobilization, J. 
Membrane Sci., 59, 39-52 (1991).

27. M. Demura, T. Komura and T. Asakura, Membrane Potential of Bombyx mori  Silk Fibroin  
Membrane Induced by an Immobilized Enzyme Reaction, Bioelectrochem. Bioenerg., 26, 167-175 (1991).

26. T. Asakura, M. Demura, M. Nagashima, R. Sakaguchi, M. Osanai and K. Ogawa, Metabolic Flux 
and Incorporation of [2-13C]Glycine into Silk Fibroin  Studied by 13C NMR in vivo and in vitro, 
Insect Biochem., 21, 743-748 (1991). 

25. T. Asakura, M. Demura, H. Ogawa, K. Matsushita and M. Imanari, NMR Imaging of Diffusion of 
Small Organic Molecules in Silk  Fibroin Gel, Macromolecules, 24, 620-622 (1991).

24. T. Asakura, E.Nakamura, H. Asakawa, M. Demura, Ring-Current Effects and Magnetic 
Anisotropy Effects of Carbonyl Groups on the α-CH Proton Chemical Shifts of the Basic 
Pancreatic  Trypsin Inhibitor and Tendamistat, J. Magn. Reson., 93, 355-360 (1991).

23. T. Asakura, M. Demura, Y. Nishiyama, Carbon-13 NMR Spectral Assignment of Five Polyolefins 
Determined from the Chemical Shift Calculation and the Polymerization Mechanism, 
Macromolecules, 24, 2334 - 2340 (1991).

22. T. Asakura, K. Hirano, M. Demura, K. Kato, 2D-INADEQUATE 13C NMR Spectra of Atactic 
Polyolefins, Makromol. Chem. Rapid Commun., 12, 215-220 (1991).

21. 北村愛夫、柴本秋男、出村誠、有機溶媒の絹への溶媒和・拡散と膨潤現象、日本蚕糸学雑誌, 59, 43-48 (1990).

20. 出村誠、香村恭史、平出啓、朝倉哲郎、リパーゼ固定化絹フィブロイン膜の酵素性能とその膜電位応答性、
繊維学会誌、46, 391-396 (1990).  

19. S. Lenka, M. Demura and T. Asakura, 13C NMR Studies of polymerization mechanism and 
dynamics of poly (alkyl methacrylate) grafted onto silk fiber, Makromol. Chem., 191, 1321-1327 
(1990). 

18. T. Asakura, H. Yamada, M. Demura and M. Osanai, 13C and 31P NMR Analysis of Cultivated 
Posterior Silkgland of the Silkworm, Bombyx mori for Silk   Fibroin Production and Effect of 
Sorbitol-6-Phosphate on the Cultivated Silkgland, Insect Biochem., 20, 261-266 (1990).

17. N. Kamo, M. Demura and Y. Kobatake, Linear equation for calibration of mitochondrial 
membrane potential, Bioelectrochem. Bioenerg., 21, 33-46 (1989).

16. M. Demura and T. Asakura, Immobilization of Glucose Oxidase with Bombyx mori Silk Fibroin by 
Only Stretching Treatment and Its Application to Glucose Sensor, Biotechnol. Bioeng., 33, 598 - 
603 (1989).

15. T. Asakura, J. Kanetake and M. Demura, Preparation and Properties of Covalently Immobilized 
Alkanine Phosphatase on Bombyx mori Silk Fibroin Fiber, Polymer-Plastic Technol. Eng., 28, 
453 - 469 (1989).

14. M. Demura, T. Asakura and E. Nakamura, Immobilization of Peroxidase with Bombyx mori Silk 
Fibroin Membrane and Its Application to Biophotosensor, J. Biotech., 10, 113-120 (1989).  

13. M. Demura, T. Asakura and T. Kuroo, Immobilization of Biocatalysts with Bombyx mori Silk 
Fibroin by Several Kinds of Physical Treatment and Its Application to Glucose sensor, 
Biosensors, 4, 361 - 372  (1989).

12. T. Asakura, M. Demura and M. Tsutsumi, 23Na and 27Al NMR Studies of the interaction between 
Bombyx mori silk fibroin and metal ions trapped in the porous silk fibroin membrane, Makromol. 
Chem. Rapid Commun., 9, 835-839 (1988).

11. T. Asakura and M. Demura, NMR of Silk Fibroin 11. 1H NMR Analysis of Water Orientation in 
Porous Silk Fibroin Membrane, Sen-i Gakkaishi, 44,535-540 (1988).

10. M. Demura, A. Kitamura, A. Shibamoto and T. Asakura, Water Sorption, Membrane Potential and 
Ion Permeability of Styrene Grafted Bombyx mori Silk Fibroin Membrane. J. Appl. Polym. Sci., 
36, 535 - 543 (1988).

  9. T. Asakura, Y. Kawaguchi, M. Demura and M. Osanai, 13C and 31P NMR studies on sugar 
metabolism in Bombyx mori and Philosamia cynthia ricini larvae, Insect Biochem.,18, 531-538 
(1988).

  8. A. Kitamura, A. Shibamoto and M. Demura, Application of silk fibroin membrane; Relationship 
between membrane preparation, membrane potential and ion permeability, Sen-i Gakkaishi, 44,193-198 (1988).

  7. M. Demura, N. Kamo and Y. Kobatake,  Mitochondrial membrane potential estimated with the 
correction of probe binding,  Biochim. Biophys. Acta, 894, 355-364 (1987).

  6. M. Demura, N. Kamo and Y. Kobatake, Binding of lipophilic cations to the liposomal membrane: 
Thermodynamic analysis, Biochim. Biophys.Acta, 903, 303-308 (1987).

  5. T. Asakura, M. Demura, K. Yamamoto and R. Chujo, Polymerization Mechanism and 
Conformation of Poly(1-butene).  Polymer, 28, 1037-1040 (1987).

  4. N. Kamo, Makoto Demura and Yonosuke Kobatake, Monitoring of cell potentials with an 
electrode sensitive to lipophilic ions, J. Membrane Science, 27, 233-239 (1986).

  3. M. Demura, N. Kamo and Y. Kobatake, Transport rate of various lipophilic ions through 
membranes of Halobacterium  halobium, Bioelectrochem. Bioenerg., 14, 439-448 (1985).

  2. M. Demura, N. Kamo and Y. Kobatake, Determination of membrane potential with lipophilic 
cations:correction of probe binding, Biochim. Biophys. Acta, 820, 207-215 (1985).

  1. M. Demura, N. Kamo and Y. Kobatake, Determination of membrane potential with lipophilic 
cations. Comparison of estimated values with various phosphonium ions, Biochim. Biophys. Acta, 
812, 377-386 (1985).