研究教育活動 | 塚本 卓 助教

 ❊ 原著論文
 ❊ 総説・解説
 ❊ 講演
 ❊ 国際会議での発表
 ❊ 詳しい業績はこちら (1.09MB・Update:2018.9.14)

原著論文

• Tsukamoto, T., Kikuchi, C., Suzuki, H., Aizawa, T., Kikukawa, T., Demura, M. (2018) "Implications for the impairment of the rapid channel closing of Proteomonas sulcata anion channelrhodopsin 1 at high Cl^- concentrations" Sci. Rep. 8, 13445. Press release here


• Shionoya, T., Mizuno, M., Tsukamoto, T., Ikeda, K., Seki, H., Kojima, K., Shibata, M., Kawamura, I., Sudo, Y., Mizutani, Y. (2018) "High Thermal Stability of Oligomeric Assemblies of Thermophilic Rhodopsin in a Lipid Environment" J. Phys. Chem. B 122(27), 6945-6953.


• Takayama, R., Kaneko, A., Okitsu, T., Tsunoda, S.P., Shimono, K., Mizuno, M., Kojima, K., Tsukamoto, T., Kandori, H., Mizutani, Y., Wada, A., Sudo, Y. (2018) "Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative" J. Am. Chem. Soc. 9(11), 2857-2862.


• Nakajima, Y., Tsukamoto, T., Kumagai, Y., Ogura, Y., Hayashi, T., Jaeho, S., Kikukawa, T., Demura, M., Kogure, K., Sudo, Y., Yoshizawa, S. (2018) "Presence of a Haloarchaeal Halorhodopsin-Like Cl^- Pump in Marine Bacteria" Microbes. Environ. 33, 89-97.


• Inoue, S., Yoshizawa, S., Nakajima, Y., Kojima, K., Tsukamoto, T., Kikukawa, T., Sudo, Y. "Spectroscopic characteristics of Rubricoccus marinus xenorhodopsin (RmXeR) and a putative model for its inward H⁺ transport mehcanism" Phys. Chem. Chem. Phys. 20, 3172-3183.


• Honda, N., Tsukamoto, T., Sudo, Y. “Comaparative evaluation of the stability of seven-transmembrane microbial rhodopsins to various physicochemical stimuli” Chem. Phys. Lett. 682, 6-14.


• Niho, A., Yoshizawa, S., Tsukamoto, T., Kurihara, M., Tahara, S., Nakajima, Y., Mizuno, M., Kuramochi, H., Tahara, T., Mizutani, Y., Sudo, Y. “Demonstration of a Light-Driven SO₄^2- Transporter and Its Spectroscopic Characteristics” J. Am. Chem. Soc. 139(12), 4376-4389.


• Tsukamoto, T.*, Yoshizawa, S., Kikukawa, T., Demura, M., Sudo, Y. (2017) “Implications for the Light-Driven Chloride Ion Transport Mechanism of Nonlabens marinus Rhodopsin 3 by Its Photochemical Characteristics” J. Phys. Chem. B 121(9), 2027-2038.


• Kanehara, K., Yoshizawa, S., Tsukamoto, T., Sudo, Y. (2017) “A phylogenetically distinctive and extremely heat stable light-driven proton pump from the eubacterium Rubrobacter xylanophilus DSM 9941^T” Sci. Rep. 7, 44427.


• Doi, S., Tsukamoto, T., Yosizawa, S., Sudo, Y. (2017) “An inhibitory role of Arg-84 in anion channelrhodopsin-2 expressed in Escherichia coli” Sci. Rep. 7, 41879.


• Tsukamoto, T., Mizutani, K., Hasegawa, T., Takahashi, M., Honda, N., Hashimoto, N., Shimono, K., Yamashita, K., Yamamoto, M., Miyauchi, S., Takagi, S., Hayashi, S., Mutara, T., Sudo, Y. (2016) “X-ray Crystallographic Structure of Thermophilic Rhodopsin: Implications for High Thermal Stability and Optogenetic Function” J. Biol. Chem. 291, 12223-12232.


• Sakamoto, A., Tsukamoto, T., Furutani, Y., Sudo, Y., Shimada, K., Tomita, A., Kiyoi, H., Kato, T., Funatsu, T. (2016) “Live-cell single-molecule imaging of the cytokine receptor MPL for analysis of dynamic dimerization”
  J. Mol. Cell Biol., 8(6), 553-555.


• Doi, S., Mori, A., Tsukamoto, T., Reissig, L., Ihara, K., Sudo, Y. (2015) “Structural and Functional Roles of the N- and C-terminal Extended Modules in Channelrhodopsin-1” Photochem. Photobiol. Sci. 14, 1628-1636.


• Kikukawa, T., Kusakabe, C., Kokubo, A., Tsukamoto, T., Kamiya, M., Aizawa, T., Ihara, K., Kamo, N., Demura, M. (2015) “Probing the Cl^-pumping Photocycle of pharaonis Halorhodopsin: Examinations with Bacterioruberin, an Intrinsic Dye, and Membrane Potential-Induced Modulation of the Photocycle” Biochim. Biophys. Acta (Bioenergetics) 1847, 748-758.


• Inoue, K., Tsukamoto, T., Shimono, K., Suzuki, Y., Miyauchi, S., Hayashi, S., Kandori, H., Sudo, Y. (2015) “Converting a Light-Driven Proton Pump into a Light-Gated Proton Channel” J. Am. Chem. Soc. 137, 3291-3299.


• Tsukamoto, T., Demura, M., Sudo, Y. (2014) “Irreversible Trimer to Monomer Transition of Thermophilic Rhodopsin upon Thermal Stimulation” J. Phys. Chem. B 118(43), 12383-12394.


• Tsukamoto, T.*, Li, X., Morita, H., Minowa, T., Aizawa, T., Hanagata, N., Demura, M. (2013) “Role of S-palmitoylation on IFITM5 for the Interaction with FKBP11 in Osteoblast Cells” PLoS ONE 8(9), e75831.


• Tsukamoto, T., Inoue, K., Kandori, H., Sudo, Y. (2013) “Thermal and Spectroscopic Characterization of a Proton Pumping Rhodopsin from an Extreme Thermophile” J. Biol. Chem. 288, pp. 21581-21592. 【Selected article on “Faculty of 1000 Biology (Microbiology)”】


• Tsukamoto, T., Kikukawa, T., Kurata, T., Jung, K.-H., Kamo, N., Demura, M. (2013) “Salt-bridge in the conserved His-Asp cluster in Gloeobacter rhodopsin contributes to trimer formation” FEBS Lett. 587, 322-327.


• Tsukamoto, T., Sasaki, T., Fujimoto, K. J., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Kamo, N., Demura, M. (2012) “Homotrimer formation and dissociation of pharaonis halorhodopsin in detergent system” Biophys. J. 102, 2906-2915.


• Yamashita, Y., Kikukawa, T., Tsukamoto, T., Kamiya, M., Aizawa, T., Kawano, K., Miyauchi, S., Kamo, N., Demura, M. (2011) “Expression of salinarum halorhodopsin in Escherichia coli cells: Solubilization in the presence of retinal yields the natural state” Biochim. Biophys. Acta (Biomembrane) 1808, 2905-2912.

総説・解説

• Tsukamoto, T., Sudo, Y. (2014) “Sensory rhodopsins” eLS (Microbiology), DOI: 10.1002/9780470015902.a0022838.


• Inoue, K., Tsukamoto, T., Sudo, Y. (2014) “Molecular and Evolutionary Aspects of Microbial Sensory Rhodopsins” Biochim. Biophys. Acta 1837, 562-577.

(Japanese articles)
• Tsukamoto, T., Sudo, Y. (2015) “Retinal Proteins in Thermophilic Bacteria” SEIBUTSU BUTSURI 55(2), 92-94.


• Mizutani, K., Hashimoto, N., Tsukamoto, T., Sudo, Y., Murata, T. (2014) “Structural Study of Bacterial Thermophilic Rhodopsin” Photon Factory Activity Report 2013 #31, B.


• Sudo, Y., Tsukamoto, T. (2014) “Solubilization of Membrane Proteins (2)” Protein Science Society of Japan Archive 7, e079.

講演

• Takashi Tsukamoto. "Photochemical Properties of Microbial Rhodopsin Pumps and Channels" (Invited talk, APSBMS 2018 Annual Meeting, July 21, 2018, Sapporo)


• 塚本卓.“好熱性バクテリアのレチナールタンパク質：発見と物性解析のこれまでとこれから”(invited talk, 第54回日本生化学会北海道支部例会（2017 年7月、札幌市）


• 塚本卓，須藤雄気. “微生物型ロドプシンの光応答性と応用技術への展開” (Invited talk)，新学術領域研究「人工光合成」若手育成シンポジウム〜生物から学び、応用する光反応〜 (2016年11月，滋賀県草津市)


• 塚本卓, 本田尚也, 西川佳吾, 林重彦, 村田武士, 須藤雄気. “高度好熱菌Thermus thermophilus 由来サーモフィリックロドプシンの物性研究” I-1-3 (Talk and Poster), 日本生物物理学会中四国支部講演会 (2016年5月, 高松市)


• 塚本卓, 須藤雄気. “高度好熱菌Thermus thermophilus由来微生物型ロドプシンの分光学的解析” T1 (Talk), P18（Poster), 2012年度生物物理学会中部支部講演会 (2013年2月, 名古屋市)


• 塚本卓, 菊川峰志, 倉田拓郎, K.-H. Jung, 加茂直樹, 出村誠. “DDM可溶化系におけるGloeobacter rhodopsinの多量体形成に関する研究” No.6 (Talk）, 日本生物物理学会2011年度北海道支部例会 (2012年3月, 旭川市)


• 塚本卓, 李香蘭, 花方信孝, 出村誠. “The role of cysteines in the first transmembrane domain of IFITM5 for the intermolecular interaction with FKBP11” 3T18a-5 (Talk & Poster), 第34回日本分子生物学会年会 (2011年12月, 横浜市)


• 塚本卓, 寺田浩太, 中川摩里恵, 田巻初, 神谷昌克, 菊川峰志, 相沢智康, 河野敬一, 出村誠. “Role of Ser171 for the stabilization of NpHR trimer” 1B1536 (Talk), 日本生物物理学会第49回年会 (2011年9月, 姫路市)


• 塚本卓, 佐々木貴規, 久保恵美, 神谷昌克, 相沢智康, 河野敬一, 出村誠. “ハロロドプシン三量体の界面活性剤中での解離” 1aC02 (Talk), 1P-091 (Poster), 日本生物物理学会第45回年会 (2007年12月, 横浜市)

国際会議での発表

• Tsukamoto, T., Kikuchi, C., Suzuki, H., Aizawa, T., Kikukawa, T., Demura, M. "Anion concentration dependency on the photocycle of PsuACR1: Implications for the impairment of its fast channel closing", (Poster talk & Poster, 18^th International Conference on Retinal Proteins, Sept. 2018, Ontario, Canada)


• Niho, A., Yoshizawa, S., Tsukamoto, T., Kurihara, M., Tahata, S., Nakajima, Y., Mizuno, M.,Kuramochi, H., Tahara, T., Mizutani, Y., Sudo, Y. “Demonstration of a light-driven SO₄^2- transporter from the cyanobacterium and its spectroscopic characterization” P-18, International Symposium on Biophysics of Rhodopsins (Poster, May, 2017, Kyoto, Japan)


• Inoue, S., Yoshizawa, S., Tsukamoto, T., Sudo, Y. “Functional and spectroscopic characterization of RmXeR, a xenorhodopsin from a marine bacterium Rubricoccus marinus SG-29^T” P-3, International Symposium on Biophysics of Rhodopsins (Poster, May,2017, Kyoto, Japan)


• Doi, S., Tsukamoto, T., Yoshizawa, S., Sudo, Y. “Functional Expression of the Eukaryotic Guillardia theta Anion Channel Rhodopsin (GtACR2) in Escherichia coli” P10, The 7^th Asia and Oceania Conference on Photobiology (Poster, Nov. 2015, Taipei, Taiwan)


• Takahashi, M., Okazaki, A., Tsukamoto, T., Sudo, Y., Takagi, S. “Behavioral analysis of C. elegans is useful for estimating availability of novel optogenetic tools” P-66, 16^th International Conference on Retinal Proteins (Poster, Oct. 2014, Nagahama, Japan)


• Reissig, L., Dalgleish, S., Tsukamoto, T., Matsushita, M., Sudo, Y., Awaga, K. “Towards Biological Photodetectors” P-52, 16th International Conference on Retinal Proteins (Poster, Oct. 2014, Nagahama, Japan)


• Mizutani, K., Hashimoto, N., Tsukamoto, T., Sudo, Y., Murata, T. “X-ray crystal structure of the proton pumping rhodopsin TR from the hyperthermophile Thermus thermophilus” P-45, 16^th International Conference on Retinal Proteins (Poster, Oct. 2014, Nagahama, Japan)


• Tsukamoto, T., Sudo, Y. “Physicochemical Characterization of a Light-Driven Proton Pump from an Extreme Thermophile” P-68, (Poster, Oct. 2014, Nagahama, Japan)


• Takahashi, M., Okazaki, A., Tsukamoto, T., Sudo, Y., Takagi, S. “Novel optogenetic tools for silencing neural activity in C. elegens” 105, Neuronal Development, Synaptic Function & Behavior C. elegans Topic Meeting 2014. (Poster, July. 2014, Madison UW, USA)


• Tsukamoto, T., Kikukawa, T., Kurata, T., Jung, K.-H., Kamo, N., Demura, M. “Homo-trimeric structure of Gloeobacter rhodopsin is regulated by the protonation state of Asp121, a counterion of the protonated Schiff base” P-54, 15^th International Conference on Retinal Proteins (Poster, Oct. 2012, Ascona, Switzerland)


• Kikukawa, T., Kokubo, A., Tsukamoto, T., Ihara, K., Kamo, N., Demura, M. “When does halorhodopsin capture and release Cl^- during photocycle? Examination by spectrum changes of an intrinsic voltage-sensitive dye, bacterioruberin” P-22, 15^th International Conference on Retinal Proteins (Poster, Oct. 2012, Ascona, Switzerland)


• Tsukamoto, T., Sasaki, T., Fujimoto, K. J., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Demura, M. “Trimer formation of N. pharaonis halorhodopsin is stabilized by the intermolecular interaction among Phe150 residues and leads to functional modulation in detergent solution” P-36, 14^th International Conference on Retinal Proteins (Poster, Aug. 2010, Santa Cruz, CA, USA)


• Tsukamoto, T., Sasaki, T., Kikukawa, T., Kamiya, M., Aizawa, T., Kawano, K., Kamo, N., Demura, M. “Trimer assembly of Natronomonas pharaonis halorhodopsin, NpHR, in lipid bilayer and detergent” P-14, 13^th International Conference on Retinal Proteins (Poster, Jun. 2008, Barcelona, Spain)