芳賀 永 HAGA Hisashi　教授

• 北海道大学 大学院先端生命科学研究院（教授）
  芳賀 永　はが　ひさし
  
  English Page座右の銘　「虚心坦懐」

経歴

• 昭和42年 北海道上川郡剣淵町生まれ
• 昭和60年 旭川東高等学校卒業
• 平成 1年 北海道大学理学部物理学科卒業
• 平成 1年 株式会社 ほくさん（現：エア・ウォーター）入社 （同年 退職）
• 平成 4年 北海道大学大学院理学研究科物理学専攻修士課程修了
• 平成 7年 北海道大学大学院理学研究科物理学専攻博士後期課程修了
• 平成 7年 米国マサチューセッツ工科大学 化学科 博士研究員
• 平成 9年 北海道大学大学院理学研究科物理学専攻 助手
• 平成14年 北海道大学大学院理学研究科生物科学専攻（高分子機能学）助教授
• 平成19年 北海道大学大学院理学研究院生命理学部門 准教授
• 平成22年 北海道大学大学院先端生命科学研究院 准教授
• 平成25年 北海道大学大学院先端生命科学研究院 教授
• 令和5年 北海道大学大学院先端生命科学研究院 研究院長

現在の研究分野

細胞生物学、腫瘍生物学、再生医学

主な研究テーマ

• 上皮細胞集団の協調運動と3次元形態形成
• がん細胞の集団浸潤運動
• 基質の硬さに誘引されるがん細胞の悪性化
• 軟らかいゲル基質を用いたES細胞の神経分化
• 細胞集団に形態形成を促す培養技術の開発

所属学会

日本メカノバイオロジー学会、日本細胞生物学会、日本癌学会

担当講義

• はじめての生命科学　（学部1年生オムニバス）
• 生物系の量子力学　（学部2年生）
• 生物系の統計力学　（学部3年生）
• ソフトマター科学概論　（大学院修士課程オムニバス）

ライフワーク

ゲーテ的自然科学、グノーシス主義、チベット仏教

趣味

ロープクライミング、登山、演劇鑑賞

研究業績

2024年

• A. Choisez, S. Ishihara, T. Ishii, Y. Xu, S. D Firouzjah, H. Haga, R. Nagatomi, and J. Kusuyama: Matrix Stiffness Regulates the Triad Communication of Adipocytes/macrophages/endothelial Cells through CXCL13, Journal of Lipid Research, 65, 100620 (2024).

2023年

• R. Kobayashi, E. Hoshikawa, T. Saito, O. Suebsamarn, E. Naito, A. Suzuki, S. Ishihara, H. Haga, K. Tomihara, and K. Izumi: The EGF/EGFR Axis and its Downstream Signaling Pathways Regulate the Motility and Proliferation of Cultured Oral Keratinocyte, FEBS Open Bio, 13, 1469–1484 (2023).
• S. Ishihara, H. Kurosawa, and H. Haga: Stiffness-Modulation of Collagen Gels by Genipin-Crosslinking for Cell Culture, Gels, 9, 148 (2023).
• K. Onishi, S. Ishihara, M. Takahashi, A. Sakai, A. Enomoto, K. Suzuki, and H. Haga: Substrate Stiffness Induces Nuclear Localization of Myosin Regulatory Light Chain to Suppress Apoptosis, FEBS Letters, 597, 643–656 (2023).

2022年

• S. Ishida-Ishihara, R. Takada, K. Furusawa, S. Ishihara, and H. Haga: Improvement of the Cell Viability of Hepatocytes Cultured in Three-dimensional Collagen Gels using Pump-free Perfusion Driven by Water Level Difference, Scientific Reports, 12, 20269, 1-11 (2022).
• R. Kusumastuti, Y. Kumagai, S. Ishihara, A. Enomoto, T. Murakami, M. Yasuda, and H. Haga: Mammaglobin 1 Mediates Progression of Trastuzumab-resistant Breast Cancer Cells through Regulation of Cyclins and NF-κB, FEBS Open Bio, 12, 1797–1813 (2022).
• R. Ichijo, K. Maki, M. Kabata, T. Murata, A. Nagasaka, S. Ishihara, H. Haga, T. Honda, T. Adachi, T. Yamamoto, and F. Toyoshima: Vasculature Atrophy Causes a Stiffened Microenvironment that Augments Epidermal Stem Cell Differentiation in Aged Skin, Nature Aging, 1-9 (2022).
• Y. Kumagai, J. Nio-Kobayashi, S. Ishihara, A. Enomoto, M. Akiyama, R. Ichihara, and H. Haga: The Interferon-β/STAT1 Axis Drives the Collective Invasion of Skin Squamous Cell Carcinoma with Sealed Intercellular Spaces, Oncogenesis, 11, 27 (2022).
• T. Iida, Y. Mizutani, N. Esaki, S. Ponik, B. Burkel, L. Weng, K. Kuwata, A. Masamune, S. Ishihara, H. Haga, K. Kataoka, S. Mii, Y. Shiraki, T. Ishikawa, E. Ohno, H. Kawashima, Y. Hirooka, M. Fujishiro, M. Takahashi, and A. Enomoto: Pharmacologic Conversion of Cancer-associated Fibroblasts from a Protumor Phenotype to an Antitumor Phenotype Improves the Sensitivity of Pancreatic Cancer to Chemotherapeutics, Oncogene, 41, 2764–2777 (2022).
• S. Ishihara and H. Haga: Matrix Stiffness Contributes to Cancer Progression by Regulating Transcription Factors, Cancers, 14, 1049 (2022).

2021年

• H. Oyama, A. Nukuda, S. Ishihara, and H. Haga: Soft Surfaces Promote Astrocytic Differentiation of Mouse Embryonic Neural Stem Cells via Dephosphorylation of MRLC in the Absence of Serum, Scientific Reports, 11, 19574, 1-11 (2021).

2020年

• S. Ishida-Ishihara, M. Akiyama, K. Furusawa, I. Naguro, H. Ryuno, T. Sushida, S. Ishihara, and H. Haga: Osmotic Gradients Induce Stable Dome Morphogenesis on Extracellular Matrix, Journal of Cell Science, 133, jcs243865 (2020).
• 石原 誠一郎、芳賀 永: 実験医学 増刊 『線維化 慢性疾患のキープロセス』, 組織の「硬さ」から迫る線維症研究の新展開, 第4章 第4節 (2020).
• T. Aizawa, M. Demura, K. Gohara, H. Haga, K. Ishimori, M. Kinjo, T. Komatsuzaki, K. Maenaka, and M. Yao: Biophysical Research in Hokkaido University, Biophysical Reviews, 12, 233–236 (2020).

2019年

• Y. Mizutani, H. Kobayashi, T. Iida, N. Asai, A. Masamune, A. Hara, N. Esaki, K. Ushida, S. Mii, Y. Shiraki, K. Ando, L. Weng, S. Ishihara, S. M. Ponik, M. W. Conklin, H. Haga, A. Nagasaka, T. Miyata, M. Matsuyama, T. Kobayashi, T. Fujii, S. Yamada, J. Yamaguchi, T. Wang, S. L. Woods, D. Worthley, T. Shimamura, M. Fujishiro, Y. Hirooka, A. Enomoto, and M. Takahashi: Meflin-positive Cancer-associated Fibroblasts Inhibit Pancreatic Carcinogenesis, Cancer Research, 79, 5367-5381 (2019).
• M. Frauenlob, D. R. King, H. Guo, S. Ishihara, M. Tsuda, T. Kurokawa, H. Haga, S. Tanaka, and J. P. Gong: Modulation and Characterization of the Double Network Hydrogel Surface-Bulk Transition, Macromolecules, 52, 6704-6713 (2019).
• Y. Kumagai, J. Nio-Kobayashi, S. Ishida-Ishihara, H. Tachibana, R. Omori, A. Enomoto, S. Ishihara, and H. Haga: The Intercellular Expression of Type-XVII Collagen, Laminin-332, and Integrin-β1 Promote Contact Following during the Collective Invasion of a Cancer Cell Population, Biochem. Biophys. Res. Commun., 514, 1115-1121 (2019).
• 石原 誠一郎、芳賀 永: 三次元培養法（ハイドロゲル法）, 『三次元培養における培養手法と周辺技術動向』 第2章 第2節 (2019).
• A. R. Acebedo, K. Suzuki, S. Hino, M. C. Alcantara, Y. Sato, H. Haga, K. Matsumoto, M. Nakao, K. Shimamura, T. Takeo, N. Nakagata, S. Miyagawa, R. Nishinakamura, R. S. Adelstein, and G. Yamada: Mesenchymal Actomyosin Contractility is Required for Androgen-driven Urethral Masculinization in Mice, Communications Biology, 2, 95 (2019).
• K. Yamamoto, K. Otomo, T. Nemoto, S. Ishihara, H. Haga, A. Nagasaki, Y. Murakami, and M. Takahashi: Differential Contributions of Nonmuscle Myosin IIA and IIB to Cytokinesis in Human Immortalized Fibroblasts, Exp. Cell Res., 376, 67-76 (2019).
• 芳賀 永: 上皮細胞シートの折り返しによる管腔形成と細胞極性, 『医学のあゆみ』 268巻、6号 507-511 (2019).

2018年

• S. Ishihara, K. Aoki, T. Mizutani, M. Amano, S. Nishimura, and H. Haga: Glycosphingolipid GM2 Induces Invasiveness in Irradiation-tolerant Lung Cancer Cells, Cell Structure and Function, 43, 177-185 (2018).
• X. Wang, A. Enomoto, L. Weng, Y. Mizutani, S. Abudureyimu, N. Esaki, Y. Tsuyuki, C. Chen, S. Mii, N. Asai, H. Haga, S. Ishida, K. Yokota, M. Akiyama, and M. Takahashi: Girdin/GIV Regulates Collective Cancer Cell Migration by Controlling Cell Adhesion and Cytoskeletal Organization, Cancer Science, 109, 3643-3656 (2018).
• I. Koh, K. Furusawa, and H. Haga: Anisotropic Multi-channel Collagen Gel (MCCG) Guides the Growth Direction of the Neurite-like Processes of PC12 Cells, Scientific Reports, 8, 13901, 1-10 (2018).
• H. Oyama, K. Takahashi, Y. Tanaka, H. Takemoto, and H. Haga: Long-term Culture of Human iPS Cell-derived Telencephalic Neuron Aggregates on Collagen Gel, Cell Structure and Function, 43, 85-94 (2018).
• 芳賀 永: 基質の軟らかさが誘引する上皮細胞の集団運動と3次元形態形成, 『生物物理』 58巻、4号 196-199 (2018).

2017年

• S. Ishihara, S. M. Ponik, and H. Haga: Mesenchymal Stem Cells in Breast Cancer: Response to Chemical and Mechanical Stimuli, Oncoscience (Editorial), 4, 158-159 (2017).
• M. Akiyama, T. Sushida, S. Ishida, and H. Haga: Conformational Mathematical Model of Collective Cell Migrations Based on Cell Polarity, Dev. Growth Differ., 59, 471-490 (2017).
• S. Imai, M. Shimamura, A. Ravankar, H. Haga, Y. Kobayashi, and Y. Yamanaka: Utilization of the Student E-Portfolio in the Graduate Education: The Case Report of “Nitobe School Program” in Hokkaido University, 6th IIAI International Congress on Advanced Applied Informatics, 147-152 (2017).

2016年

• A. Sakane, S. Yoshizawa, M. Nishimura, Y. Tsuchiya, N. Matsushita, K. Miyake, K. Horikawa, I. Imoto, C. Mizuguchi, H. Saito, T. Ueno, S. Matsushita, H. Haga, S. Deguchi, K. Mizuguchi, and H. Yokota: Conformational Plasticity of JRAB/MICAL-L2 Provides ‘Law and Order’ in Collective Cell Migration, Molecular Biology of the Cell, 27, 3095-3108 (2016).
• A. Nukuda, H. Endoh, M. Yasuda, T. Mizutani, K. Kawabata, and H. Haga: Role of ATF5 in the Invasive Potential of Diverse Human Cancer Cell Lines, Biochem. Biophys. Res. Commun., 474, 509–514 (2016).
• T. Mizutani, K. Furusawa, H. Haga, K. Kawabata: Heterogeneous Filament Network Formation by Myosin Light Chain Isoforms Effects on Contractile Energy Output of Single Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells, Regenerative Therapy, 3, 90-96 (2016).
• T. Yamada, M. Tsuda, T. Wagatsuma, Y. Fujioka, M. Fujioka, A. O. Satoh, K. Horiuchi, S. Nishide, A. Nanbo, Y. Totsuka, H. Haga, S. Tanaka, M. Shindoh, and Y. Ohba: Receptor Activator of NF-κB Ligand Induces Cell Adhesion and Integrin α2 Expression via NF-κB in Head and Neck Cancers, Scientific Reports, 6, 23545, 1-16 (2016).
• T. Mizutani, H. Haga, and K. Kawabata: Data Set for Comparison of Cellular Dynamics between Human AAVS1 Locus-modified and Wild-type Cells, Data in Brief, 6, 793–798 (2016).
• S. Ishihara, T. Mizutani, K. Kawabata, and H. Haga: An Improved Method for Western Blotting When Extracting Proteins from Mammalian Cells Cultured on a Collagen Gel under Serum-free Conditions, Cytotechnology, 68, 25-32 (2016).

2015年

• M. Imai, K. Furusawa, T. Mizutani, K. Kawabata, and H. Haga: Three-dimensional Morphogenesis of MDCK Cells Induced by Cellular Contractile Forces on a Viscous Substrate, Scientific Reports, 5, 14208, 1-10 (2015).
• T. Mizutani, R. Li, H. Haga, and K. Kawabata: Transgene Integration into the Human AAVS1 Locus Enhances Myosin II-Dependent Contractile Force by Reducing Expression of Myosin Binding Subunit 85, Biochem. Biophys. Res. Commun., 465, 270-274 (2015).
• A. Nukuda, C. Sasaki, S. Ishihara, T. Mizutani, K. Nakamura, T. Ayabe, K. Kawabata, and H. Haga: Stiff Substrates Increase YAP-Signaling-Mediated Matrix Metalloproteinase-7 Expression, Oncogenesis, 4, e165 (2015).
• Y. Iguchi, S. Ishihara, Y. Uchida, K. Tajima, T. Mizutani, K. Kawabata, and H. Haga: Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices, Cell Structure and Function, 40, 61-67 (2015).
• K. Takemoto, S. Ishihara, T. Mizutani, K. Kawabata, and H. Haga: Compressive Stress Induces Dephosphorylation of the Myosin Regulatory Light Chain via RhoA Phosphorylation by the Adenylyl Cyclase/Protein Kinase A Signaling Pathway, PLOS ONE, 10, e0117937 (2015).
• S. Ishihara, M. Yasuda, A. Ishizu, M. Ishikawa, H. Shirato, and H. Haga: Activating Transcription Factor 5 Enhances Radioresistance and Malignancy in Cancer Cells, Oncotarget, 6, 4602-4614 (2015).
• N. Yamaguchi, T. Mizutani, K. Kawabata, and H. Haga: Leader Cells Regulate Collective Cell Migration via Rac Activation in the Downstream Signaling of Integrin Beta1 and PI3K, Scientific Reports, 5, 7656, 1-8 (2015).

2014年

• S. Ishida, R. Tanaka, N. Yamaguchi, G. Ogata, T. Mizutani, K. Kawabata, and H. Haga: Epithelial Sheet Folding Induces Lumen Formation by Madin-Darby Canine Kidney Cells in a Collagen Gel, PLOS ONE, 9, e99655 (2014).
• T. Kato, A. Enomoto, T. Watanabe, H. Haga, S. Ishida, Y. Kondo, K. Furukawa, S. Mii, L. Weng, M. Ishida-Takagishi, M. Asai, N. Asai, K. Kaibuchi, Y. Murakumo, and M. Takahashi: TRIM27/MRTF-B-dependent Integrin β1 Expression Defines Leading Cells in Cancer Cell Collectives, Cell Reports, 7, 1156-1167 (2014).
• H. Masuda, S. Ishihara, I. Harada, T. Mizutani, M. Ishikawa, K. Kawabata, and H. Haga: Coating Extracellular Matrix Proteins on a (3-Aminopropyl)Triethoxysilane-treated Glass Substrate for Improved Cell Culture, BioTechniques, 56, 172-179 (2014).
• T. Mizutani, K. Takeda, H. Haga, M. Todo, and K. Kawabata: Modulation of Extracellular Conditions Prevents the Multilayering of the Simple Epithelium, Histochem. Cell Biol., 141, 473-481 (2014).

2013年

• S. Ishihara, M. Yasuda, I. Harada, T. Mizutani, K. Kawabata, and H. Haga: Substrate Stiffness Regulates Temporary NF-κB Activation via Actomyosin Contractions, Exp. Cell Res., 319, 2916-2927 (2013).
• X. Li, S. Ishihara, M. Yasuda, T. Nishioka, T. Mizutani, M. Ishikawa, K. Kawabata, H. Shirato, and H. Haga: Lung Cancer Cells that Survive Ionizing Radiation Show Increased Integrin α2β1- and EGFR-dependent Invasiveness, PLOS ONE, 8, e70905 (2013).
• S. Ishihara, M. Yasuda, T. Nishioka, T. Mizutani, K. Kawabata, H. Shirato, and H. Haga: Irradiation-tolerant Lung Cancer Cells Acquire Invasive Ability Dependent on Dephosphorylation of the Myosin Regulatory Light Chain, FEBS Letters, 587, 732-736 (2013).
• R. Tanaka, T. Mizutani, H. Haga, and K. Kawabata: Tempo-Spatial Change of Cellular Stiffness and Geometry in the Process of Developing Epithelial Cell-Cell Adhesion Measured by Atomic Force Microscopy, Jpn. J. Appl. Phys. Conf. Proc., 1, 011004 (2013).

2012年

• K. Takemoto, T. Mizutani, K. Tamura, K. Takeda, H. Haga, and K. Kawabata: The number of cyclic stretch regulates cellular elasticity in C2C12 myoblasts, Open Journal of Cell Biology, 2, 1-10 (2012).
• N. Seito, T. Yamashita, Y. Tsukuda, Y. Matsui, A. Urita, T. Onodera, T. Mizutani, H. Haga, N. Fujitani, Y. Shinohara, A. Minami, and N. Iwasaki: Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice, Arthritis & Rheumatism, 6, 2579-2588 (2012).

2011年

• K. Tamura, T. Mizutani, H. Haga, and K. Kawabata: Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy, Acta Biomater., 7, 3766-3772 (2011).
• T. Nishioka, M. Yasuda, T. Takeshima, H. Haga, Y. Miyai, K. Shibata, R. Yamazaki, H. Shirato, M. Teduka, and H. Date: Radiation-induced Cancer Cell Repopulation: A Possible Mechanism Implied by Experiments Using Transplantable Mouse-derived Sarcoma Cell Line, Cell Structure and Function, 36, 13-20 (2011).

2010年

• K. Tamura, T. Mizutani, H. Haga, and K. Kawabata: Nano-mechanical Properties of Living Cells Expressing Constitutively Active RhoA Effectors, Biochem. Biophys. Res. Commun., 403, 363-367 (2010).
• H. Suzuki, H. M. Nurul, T. Seki, T. Kawamoto, H. Haga, K. Kawabata, and Y. Takeoka: Precise Synthesis and Physicochemical Properties of High-Density Polymer Brushes Designed with Poly(N-isopropylacrylamide), Macromolecules, 43, 9945-9956 (2010).
• K. Ikeda, T. Mizutani, O. Hoshi, T. Ushiki, H. Haga, and K. Kawabata: Intermediate Structure Between Chromatin Fibers and Chromosome Revealed by Mechanical Stretching and SPM Measurement, Biochem. Biophys. Res. Commun., 400, 181-186 (2010).
• S. Ishihara, H. Haga, M. Yasuda, T. Mizutani, K. Kawabata, H. Shirato, and T. Nishioka: Integrin β1-dependent Invasive Migration of Irradiation-tolerant Human Lung Adenocarcinoma Cells in 3D Collagen Matrix, Biochem. Biophys. Res. Commun., 396, 651-655 (2010).
• K. Tamura, T. Mizutani, H. Haga, and K. Kawabata: Dynamics of Leading Lamellae of Living Fibroblasts Visualized by High-speed Scanning Probe Microscopy, Histochem. Cell Biol., 133, 59-67 (2010).

2009年

• K. Tsutsumi, M. Tsuda, N. Yazawa, H. Nakamura, S. Ishihara, H. Haga, M. Yasuda, R. Yamazaki, H. Shirato, H. Kawaguchi, T. Nishioka, and Y. Ohba: Increased Motility and Invasiveness of Tumor Cells that Survived 10 Gy Irradiation, Cell Structure and Function, 34, 89-96 (2009).
• T. Mizutani, K. Kawabata, Y. Koyama, M. Takahashi, and H. Haga: Regulation of Cellular Contractile Force in Response to Mechanical Stretch by Diphosphorylation of Myosin Regulatory Light Chain via RhoA Signaling Cascade, Cell Motil. Cytoskeleton, 66, 389-397 (2009).
• T. Nishioka, Y. Miyai, H. Haga, K. Kawabata, H. Shirato, A. Homma, K. Shibata, and Motoaki Yasuda: Novel Function of Transcription Factor ATF5: Blockade of p53-dependent Apoptosis Induced by Ionizing Irradiation, Cell Structure and Function, 34, 17-22 (2009).

2008年

• 水谷 武臣、芳賀 永、加藤 幸作、川端 和重: 走査型プローブ顕微鏡による細胞機能の解析, 『細胞』 40巻、6月号 32-35 (2008).
• T. Kawamoto, H. Haga, K. Tamura, T. Mizutani, and K. Kawabata: Mechanical Response to Isotropic Shrinkage of Fibroblasts Measured by Scanning Probe Microscopy, Jpn. J. Appl. Phys., 47, 6173-6176 (2008).
• 芳賀 永、水谷 武臣、川端 和重: 走査型プローブ顕微鏡を用いた生細胞表面の弾性分布測定, 『表面科学』 29巻、4号 235-238 (2008).

2007年

• T. Nishioka, H. Haga, Y. Miyai, M. Yasuda, K. Tsutsumi, R. Yamazaki, H. Shirato, and K. Kawabata: Three-Dimensional Tumor Cell Movement in Collagen Gel: Mesenchymal-to-Amoeboid Transition in a Sub-Clone that Survived 10 Gy Irradiation, Int. J. Radiat. Oncol. Biol. Physics, 69(S), 148 (2007).
• K. Tamura, T. Mizutani, H. Haga, and K. Kawabata: Visualization of stretch-induced intracellular tensional response of single fibroblasts by mechanical scanning probe microscopy, Jpn. J. Appl. Phys., 46, 5631-5635 (2007).
• H. Haga and K. Kawabata: Collective Movement and Morphogenesis of Epithelial Cells, Proceedings of the international symposium on topological aspects of critical systems and networks, 82-85 (2007).
• T. Mizutani, H. Haga, and K. Kawabata: Development of a Wielding Device to Stretch Tissue-like Materials for Mechanical Measurements of Cell Sizes by Using Scanning Probe Microscopy, Acta Biomater., 3, 485-493 (2007).
• 芳賀 永: 細胞内部の力を可視化するナノフォース走査型プローブ顕微鏡の開発, 『バイオとナノの融合 －新生命科学の基礎－』第9章 115-125 (2007).
• H. Haga, M. Nagayama, and K. Kawabata: Imaging Mechanical Properties of Living Cells by Scanning Probe Microscopy (Short Review), Curr. Nanosci., 3, 97-103 (2007).
• 芳賀 永: 細胞が生み出す「力」を見る, 月刊『バイオニクス』 2月号 70-73 (2007).

2006年

• T. Mizutani, H. Haga, Y. Koyama, M. Takahashi, and K. Kawabata: Diphosphorylation of the Myosin Regulatory Light Chain Enhances the Tension Acting on Stress Fibers in Fibroblasts, J. Cell. Physiol., 209, 726-731 (2006).
• K. Kato, Y. Ohmori, T. Mizutani, H. Haga, K. Ohashi, T. Ito, and K. Kawabata: The Role of Actin-Binding Protein Filamin A in Cellular Stiffness and Morphology Studied by Wide-Range Scanning Probe Microscopy, Jpn. J. Appl. Phys., 45, 2328-2332 (2006).
• T. Mizutani, H. Haga, K. Kato, K. Matsuda, and K. Kawabata: Observation of Stiffer Domain Structure on Collagen Gels Using Wide-Range Scanning Probe Microscopy, Jpn. J. Appl. Phys., 45, 2535-2356 (2006).

2005年

• T. Nitta, H. Kato, H. Haga, K. Nemoto, and K. Kawabata: Static Friction of Agar Gels: Formation of Contact Junctions at Frictional Interface, J. Phys. Soc. Jpn., 74, 2875-2879 (2005).
• K. Nomura, O. Hoshi, D. Fukushi, T. Ushiki, H. Haga, and K. Kawabata: Visualization of Elasticity Distribution of Single Human Chromosomes by Scanning Probe Microscopy, Jpn. J. Appl. Phys., 44, 5421-5424 (2005).
• S. Toko, T. Mizutani, H. Haga, and K. Kawabata: Spatiotemporal Variation in Cellular Stiffness Corresponding to Development of Morphological Polarity, Jpn. J. Appl. Phys., 44, 5451-5454 (2005).
• 水谷 武臣、芳賀 永、川端 和重: 広域走査型プローブ顕微鏡の開発と細胞生物学への応用, 『生物物理』 45巻、2号 105-108 (2005).
• H. Haga, C. Irahara, R. Kobayashi, T. Nakagaki, and K. Kawabata: Collective Movement of Epithelial Cells on a Collagen Gel Substrate, Biophys. J., 88, 2250-2256 (2005).

2004年

• T. Mizutani, H. Haga, and K. Kawabata: Cellular Stiffness Response to External Deformation: Tensional Homeostasis in a Single Fibroblast, Cell Motil. Cytoskeleton, 52, 242-248 (2004).
• M. Nagayama, H. Haga, M. Takahashi, T. Saitoh, and K. Kawabata: Contribution of Cellular Contractility to Spatial and Temporal Variations in Cellular Stiffness, Exp. Cell Res., 300, 396-405 (2004).
• T. Mizutani, H. Haga, K. Nemoto, and K. Kawabata: Wide-Range Scanning Probe Microscopy for Visualizing Biomaterials in a Sub-Millimeter Range, Jpn. J. Appl. Phys., 43, 4525-4528 (2004).
• 水谷 武臣、芳賀 永、川端 和重: 細胞のダイナミクスと集団運動, 『物性研究』 83巻、3号 333-334 (2004).

2003年

• T. Nitta, Y. Endo, H. Haga, and K. Kawabata: Microdomain Structure of Agar Gels Observed by Mechanical-Scanning Probe Microscopy, J. Electron. Microsc., 52, 277-281 (2003).
• K. Kawabata, Y. Sado, M. Nagayama, T. Nitta, K. Nemoto, Y. Koyama, and H. Haga: Visualization of Dynamic Organization of Cytoskeleton Gels in Living Cells by Hybrid-SPM, Chinese J. Polym. Sci., 21, 169-174 (2003).
• 永山 昌史, 芳賀 永、田中　芳雄、平井　義彦、川端　和重: 走査プローブ顕微鏡の生物学への応用・細胞のマイクロ粘弾性, 『電子顕微鏡』 38巻、2号 90-93 (2003).

2002年

• M. Nagayama, H. Haga, Y. Tanaka, Y. Hirai, M. Kabuto, and K. Kawabata: Improvement of Force Modulation Mode with Scanning Probe Microscopy for Imaging Viscoelasticity of Living Cells, Jpn. J. Appl. Phys., 41, 4952-4955 (2002).
• T. Nitta, H. Haga, and K. Kawabata: Time Dependent Static Friction Force of Agar Gel-on-Glass Plate Immersed in Water, J. Phys. IV, 12, 319-320 (2002).
• 川端 和重, 佐戸 康洋, 永山 昌史, 芳賀 永: ハイブリット走査型プローブ顕微鏡による生細胞の力学的観察, 『光学 (Jpn. J. Optics) 』 31巻、6号 483-488 (2002).

2001年

• K. Kawabata, M. Nagayama, H. Haga, and T. Sambongi: Mechanical Effects on Collective Phenomena of Biological Systems: Cell Locomotion, Curr. Appl. Phys., 1, 66-71 (2001).
• M. Nagayama, H. Haga, and K. Kawabata: Drastic Change of Local Stiffness Distribution Correlating to Cell Migration in Living Fibroblasts, Cell Motil. Cytoskeleton, 50, 173-179 (2001).
• T. Saitoh, S. Takemura, K. Ueda, H. Hosoya, M. Nagayama, H. Haga, K. Kawabata, A. Yamagishi, and M. Takahashi: Differential Localization of Non-muscle Myosin II Isoforms and Phosphorylated Regulatory Light Chains in Human MRC-5 Fibroblasts, FEBS Letters, 509, 365-369 (2001).

2000年

• H. Haga, S. Sasaki, K. Kawabata, E. Ito, T. Ushiki, and T. Sambongi: Elasticity Mapping of Living Fibroblasts by AFM and Immunofluorescence Observation of Cytoskeleton, Ultramicroscopy, 82, 253-258 (2000).
• T. Nitta, H. Haga, K. Kawabata, K. Abe, and T. Sambongi: Comparing Microscopic with Macroscopic Elastic Properties of Polymer Gel, Ultramicroscopy, 82, 223-226 (2000).
• H. Shiga, Y. Yamane, E. Ito, K. Abe, K. Kawabata, and H. Haga: Mechanical Properties of Membrane Surface of Cultured Astrocyte Revealed by Atomic Force Microscopy, Jpn. J. Appl. Phys., 39, 3711-3716 (2000).
• K. Kawabata, H. Haga, T. Nitta, Y. Endo, M. Nagayama, E. Ito, and T. Sambongi: Big Softer Hole on Living Cell: Elasticity Imaging with AFM, Scanning and Force Microscopies for Biomedical Applications II, Proceedings of SPIE, 3922, 91-98 (2000).
• Y. Yamane, H. Shiga, H. Haga, K. Kawabata, K. Abe, and E. Ito: Quantitative Analyses of Topography and Elasticity of Living and Fixed Astrocytes, J. Electron. Microsc., 49, 463-471 (2000).
• H. Haga, M. Nagayama, K. Kawabata, E. Ito, T. Ushiki, and T. Sambongi: Time-Lapse Viscoelastic Imaging of Living Fibroblasts Using Force Modulation Mode in AFM, J. Electron. Microsc., 49, 473-481 (2000).
• T. Tojima, Y. Yamane, H. Takagi, T. Takeshita, T. Sugiyama, H. Haga, K. Kawabata, T. Ushiki, K. Abe, T. Yoshioka, and E. Ito: Three-dimensional characterization of interior structures of exocytotic apertures of nerve cells using atomic force microscopy, Neuroscience, 101, 471-481 (2000).
• 芳賀 永、川端 和重: 細胞運動に伴う力学構造のAFM粘弾性測定, 『電子顕微鏡』 35巻、3号 276-278 (2000).

1999年

• Y. Yamane, D. Hatakeyama, H. Haga, K. Abe, and E. Ito: Structures of Cultured Type 2 Astrocytes Revealed by Atomic Force Microscopy, Zoological Science, 16, 1-7 (1999).
• 川端 和重、芳賀 永: 原子間力顕微鏡をもちいた培養細胞の粘弾性測定, 『生物物理』 39巻、3号 179-181 (1999).
• H. Haga, S. Sasaki, K. Kawabata, E. Ito, K. Abe, and T. Sambongi: Spatial and Temporal Viscoelastic Imaging of Living Fibroblasts by Atomic Force Microscopy, Biological Physics: Third International Syposium, CP 487, 229-234 (1999).
• K. Kawabata, M. Morimoto, H. Ishizuka, T. Nitta, H. Haga, E. Ito, K. Abe, T. Ushiki, and T. Sambongi: Time Dependent Viscoelastic Image of Living Nerve Cells Using AFM, Biological Physics: Third International Syposium, CP 487, 235-239 (1999).
• Y. Yamane, H. Shiga, H. Asou, H. Haga, K. Kawabata, K. Abe, and E. Ito: Dynamics of Adhesion of Astrocytes Analyzed by a Combination of Atomic Force Microscopy and Immunocytochemistry, Arch. Histol. Cytol., 62, 355-361 (1999).
• P. Gorria, P. Barois, H. T. Nguyen, G. Sigaud, L. Navailles, C. W. Garland, and H. Haga: Thermal and Structural Study Close to a SmecticA-SmecticA Critical Point, Molecular Crystals & Liquid Crystals 330, 1419-1426 (1999).
• T. Hosono, M. Yamanaka, T. Tojima, Y. Yamane, H. Sadamoto, D. Hatakeyama, H. Haga, K. Kawabata, K. Abe, and E. Ito: Atomic Force Microscopic Observation of Three-Dimensional Morphological Changes of Neurons when Stimulated by a Neurotransmitter, Jpn. J. Appl. Phys., 38, 3940-3945 (1999).
• 川端 和重、芳賀 永、三本木 孝: 原子間力顕微鏡による生きた細胞の粘弾性マッピング, 『日本バイオレオロジー学会誌』 13巻、3号 1-9 (1999).

1998年

• H. Haga and C. W. Garland: Calorimetric study of the smectic-A－hexatic-B transition in 3(10)OBC, Phys. Rev., E57, 603-609 (1998).
• S. Sasaki, M. Morimoto, H. Haga, K. Kawabata, E. Ito, T. Ushiki, K. Abe, and T. Sambongi: Elastic Properties of Living Fibroblasts as Imaged Using Force Modulation Mode in Atomic Force Microscopy, Arch. Histol. Cytol., 61, 57-63 (1998).
• H. Haga, S. Sasaki, M. Morimoto, K. Kawabata, E. Ito, K. Abe, and T. Sambongi: Imaging Elastic Properties of Soft Materials Immersed in Water Using Force Modulation Mode in Atomic Force Microscopy, Jpn. J. Appl. Phys., 37, 3860-3863 (1998).

1997年

• H. Haga and C. W. Garland: Calorimetric Study of Heptyloxybenzylidene Butylaniline in Silica Aerogels, Liquid Crystals, 22, 275-277 (1997).
• H. Haga, Z. Kutnjak, G. S. Iannacchione, S. Qian, D. Finotello, and C. W. Garland: Quasicritical Heat Capacity at a Smectic-A－Hexatic-B Phase Transition, Phys. Rev., E56, 1808-1818 (1997).
• H. Haga and C. W. Garland: Effect of Silica Aerosil Particles on Liquid Crystal Phase Transition, Phys. Rev., E56, 3044-3052 (1997).
• F. Beaubois, T. Claverie, J. P. Marcerou, J. C. Rouillon, H. T. Nguyen, C. W. Garland, and H. Haga: Influence of Nematic Range on Birefringence, Heat Capacity, and Elastic Modulus near a Nematic－Smectic-A Phase Transition, Phys. Rev., E56, 5566-5574 (1997).
• H. Haga and C. W. Garland: Thermal Study of the Influence of Aerosils on the Phase Transitions of Heptyloxybenzylidene Butylaniline, Liquid Crystals, 23, 645-652 (1997).

1996年

• N. Noda, H. Haga, R. Nozaki, and Y. Shiozaki: A Calorimetric Study of [Rochelle Salt](1-x)-[Ammonium Rochelle Salt](x) Mixed Crystal System, Ferroelectrics, 184, 293-296 (1996).
• 芳賀 永、江間 健司: Rb2ZnCl4系強誘電体の正常－不整合相転移における３次元ＸＹ的臨界熱異常, 『固体物理』 31巻、8号 687-695 (1996).
• N. Noda, H. Nakano, H. Haga, R. Nozaki, and Y. Shiozaki: Evaluation of the Specific Heat Anomaly of RS(1-x)-ARS(x), J. Korean Phys. Soc., 29, S513-S516 (1996).
• R. Nozaki, H. Nakano, N. Noda, H. Haga, and Y. Shiozaki: A Study of Dynamic Heat Capacity with AC Calorimetry, J. Korean Phys. Soc., 29, S736-S739 (1996).

1995年

• N. Noda, H. Haga, T. Kikuta, R. Nozaki, and Y.Shiozaki: A Calorimetric Study of RS(1-x)-ARS(x) Mixedcrystalsystem(0.9<=x<=1.0), Ferroelectrics, 168, 169-175 (1995).
• H. Haga, A. Onodera, Y. Shiozaki, K. Ema, and H. Sakata: Three-Dimensional XY Critical Behavior of Heat Capacity at Normal-Incommensurate Phase Transition in Rb2ZnCl4, J. Phys. Soc. Japan, 64 (3), 822-829 (1995).
• H. Haga, R. Nozaki, Y. Shiozaki, and K. Ema: Preasymptotic 3D XY Critical Behavior of Heat Capacity at Normal Incommensurate Phase Transition in K2ZnCl4 and K2SeO4, J. Phys. Soc. Japan, 64 (11), 4258-4264 (1995).

1994年

• A. Onodera, H. Haga, F. Denoyer, J. Godart, and Y. Shiozaki: Specific Heat and Critical Behavior in Thiourea at Normal-INC Phase Transition, Ferroelectrics, 155, 305-310 (1994).
• H. Haga, A. Onodera, H. Yamashita, and Y. Shiozaki: Thermal and Dielectric Studies on Ferroelectric (CH3NHCH2COOH)3CaCl2 (TSCC), Ferroelectrics, 159, 55-60 (1994).
• A. Onodera, O. Watanabe, H. Yamashita, H. Haga, and Y. Shiozaki: Kinetic Evolution in the Incommensurate Phase in K2ZnCl4, Ferroelectrics, 155, 299-304 (1994).
• A. Onodera, O. Watanabe, H. Yamashita, H. Haga, and Y. Shiozaki: Dielectric Behavior and Relaxation Process in Modulated Phases in Thiourea, Ferroelectrics, 155, 311-316 (1994).

1993年

• H. Haga, A. Onodera, M. Tokunaga, and Y. Shiozaki: Critical Behaviour of Specific Heat in Ferroelectric K2ZnCl4 and (NH4)2BeF4 at Normal-Incommensurate Phase Transition, J. Phys. Soc. Japan, 62 (5), 1597-1602 (1993).
• H. Haga, A. Onodera, H. Yamashita, and Y. Shiozaki: New phase Transition in Ferroelectric (CH3NHCH2COOH)3CaCl2 at Low Temperatures, J. Phys. Soc. Japan, 62 (6), 1857-1859 (1993).
• A. Onodera, B. A. Strukov, A. A. Belov, S. A. Taraskin, H. Haga, H. Yamashita, and Y. Uesu: Thermal and Dielectric Properties of a New Ferroelectric LaBGeO5, J. Phys. Soc. Japan, 62 (12), 4311-4315 (1993).

1992年

• H. Haga, A. Onodera, and Y. Shiozaki: Specific Heat and Critical Indices Associated with Normal-Incommensurate Phase Transition in K2ZnCl4 and (NH4)2BeF4, Ferroelectrics, 125, 123-128 (1992).
• A. Onodera, O. Watanabe, H. Haga, T. Kikuta, E. Suzuki, H. Yamashita, and Y. Shiozaki: Dielectric Properties and Phase Transition in Cesium Tetrachlorozincate Cs2ZnCl4, Ferroelectrics, 125, 141-146 (1992).