Laboratory of Biological Information Analysis Science
Study on structure-function relationship of biological molecules in the biomembrane system based on experimental and information sciences
Membrane protein, Silk, Peptide, Biomimicry, Folding, NMR, Photobiology, Rhodopsin, Apoptosis, Bioinformatics
- Structure and function of rhodopsin family
The seven transmembrane (7TM) receptors have a remarkable similarity to bacteriorhodopsin and halorhodopsin (NpHR). These are a light driven proton pump and a light driven chloride pump from the archaebacteria. The 7TM segments enclose a retinal attached to a lysine residue of the last helix by a Schiffs base. This retinal undergoes an all-trans to 13-cis isomerization when it absorbs a photon. The conformational change is transmitted to the protein and it is used to pump protons or chloride and form a gradient. In vertebrate eyes, the protein rhodopsin is also a 7TM segment protein with retinal attached to a lysine also in the last helix. In this protein the photo-conformational change activates a signal transducer. We are investigated mechanism of ion pumping and signal transduction of archaeal rhodopsins using a transgenic technology and various spectroscopies including CD, flash-photolysis, and NMR.
- Protein folding and induction of apoptosis
Water soluble protein from milk changes 3D-structure from native to denatured via structural intermediates such as molten globule state when denaturant is added or temperature is changed. Does the unfolded protein from native state have biological function? We found that conformational trap of the milk protein at the molten globule state induced an apoptosis activity although the original enzymatic function of milk protein is lost at the molten globule state. The molecular mechanism of this apoptosis of tumor cell with the milk protein is investigating.
- Determination and prediction of protein structure using NMR and bioinformatics
The lifescience in recent years has been transfigured to the science that handles huge information by the result of the genome analysis project. It is very important to develop how to process these huge information and apply to an individual research of biomolecules such as protein, nucleic acid and biomembrane. On the other hand, advanced solution and solid state NMR techniques for determination of protein structure have been developed. Based on these experimental and information sciences, the methodology for determination and simulation of protein structure is developed.
- Industrial application of protein and peptide to regenerative medicine
Based on biological function and structural information, industrial application of protein and peptide to regenerative medicine such as nerve and skin are in progress.