The SELEX (Systematic Evolution of Ligands by EXponential enrichment) procedure explores the consensus sequence for nucleic acid binding protein through repeated cycles of binding selection and PCR amplification. The result of SELEX analysis provides significant information into the functions of putative transcriptional regulators. In the standard SELEX procedure, the original random sequence pool for selection is generated by PCR with synthesized oligonucleotides containing a random sequence region as a template. In genomic SELEX, however, the sequence library is derived from the chromosomal DNA of the target organism. Hence, genomic SELEX can directly determine the binding position of the target protein on its genome sequence. We have determined several operator elements of transcriptional regulators by SELEX and genomic SELEX analyses.
References