9th Japan-Korea Bilateral Symposium on Biological NMR

 

Date: March 16, 2012

Place: Hokkaido University Conference Hall

Registration fee: Free

Organizers: 9th Japan-Korea Bilateral Symposium on Biological NMR Executive Committee

Contact Address:

 

Program DOWNLOAD (PDF)

Poster DOWNLOAD (PDF)

 

Program

 

9:00-9:10             Opening remarks

 

9:10-9:30             Tomoyasu Aizawa (Hokkaido University)

Development of novel expression systems for NMR structural analysis of antimicrobial peptides

9:30-9:50             Atsushi Matsuura (Kookmin University)

Functional Identification of Toxin-Antitoxin Molecules from Helicobacter pylori 26695 and Structural Elucidation of the Molecular Interactions

9:50-10:10           Bong-Jin Lee (Seoul National University)

Structural Insights into the Binding of AIP4 WW2 domain with LMP2A PY motifs of Epstein-Barr Virus

10:10-10:30         Takahisa Ikegami (Osaka University)

Is the interaction site with SH2 domains really well-known several residues including phosphotyrosine alone?

 

10:30-10:50         Coffee break

 

10:50-11:10         Chojiro Kojima (Osaka University)

Discovery of flowering hormone receptor by NMR

11:10-11:30         Tadayasu Ohkubo (Osaka University)

NMR Solution Structure of Lipocalin-type Prostaglandin D Synthase

11:30-12:10         Mitsuhiko Ikura (University of Toronto)

Probing cancer cell signaling by NMR: structure, interaction, and enzymatic kinetics of small GTPases

 

12:10-13:30         Poster session / Lunch

 

13:30-13:50         Hiroshi Matsuo (University of Minnesota)

Studying HIV-1restriction factor APOBEC3G using NMR

13:50-14:10         Masato Katahira (Kyoto University)

Sliding-direction-dependent activity of anti-HIV enzyme, and structural basis of anti-prion activity of RNA aptamer

14:10-14:30         Suhkmann Kim (Busan National University)

Metabolomics and its Application using High-Resolution Magic Angle Spinning NMR

14:30-14:50         Hidehito Tochio (Kyoto University)

In situ analysis of proteins in eukaryotic cells by magnetic resonance

 

14:50-15:10         Coffee break

 

15:10-15:30         Makoto Demura (Hokkaido University)

Solid-State NMR Study of Seven-Transmembrane Microbial Rhodopsin

15:30-15:50         Toshimichi Fujiwara (Osaka University)

Solid-State NMR Structural Analysis of Membrane Protein and Sensitivity Enhancement by Dynamic Nuclear Polarization

15:50-16:10         Su-Jin Kang (Seoul National University)

Solid-state NMR analysis of H+-ATP synthase subunit c-ring

from Thermophilic Bacillus

16:10-16:30         Hideo Akutsu (Osaka University, Seoul National University)

Molecular Basis of the Energy Conversion by Proton ATPsynthase Elucidated by NMR

 

16:30-16:50         Coffee break

 

16:50-17:10         Sung-Jean Park (Gachon University)

Dynamic Interaction of Hsp90 with its Client Protein p53

17:10-17:30         Mineyuki Mizuguchi (University of Toyama)

Structural changes of the intrinsically disordered protein PQBP-1

17:30-17:50         Jeong-Yong Suh (Seoul National University)

Impact of Charge Reversal Mutation on Equilibrium Binding and Transient Encounter Complex Formation of a Phosphotransferase Protein Complex

17:50-18:10         Keiichi Kawano (Hokkaido University)

Surveying Structures of Biological Macromolecules by NMR

 

18:10-18:20         Closing remarks

 

 

Poster session  (12:10-13:30)

 

P01  Towards evaluation of wood cell wall structures and those biodegradation using solution NMR

Hiroshi Nishimura1, Yu Kozawa1, Hideyasu Okamura1, Takashi Watanabe2, Masato Katahira1

1Institute of Advanced Energy, Kyoto University, 2Res. Inst. for Sustainable Humanosphere, Kyoto Univ.

 

P02  Recognition of Prion Protein by the RNA Aptamer

Tsukasa Mashima1, Hiromichi Fujiwara1, Fumiko Nishikawa2, Masayuki Saimura1, Yuji O. Kamatari3, Kazuo Kuwata3, Morikazu Imamura4, Takashi Yokoyama4, Satoshi Nishikawa3 and Masato Katahira1

1Institute of Advanced Energy, Kyoto University, 2National Institute of Advanced Industrial Science and Technology, 3Center for Emerging Infectious Diseases, Gifu University, 4Prion Disease Research Center, National Institute of Animal Health

 

P03  Solution Structure of Hypothetical Protein HP1423 reveals the Presence of alpha L motif related to RNA binding

Ye-Ji Bae

Seoul National University

 

P04  Backbone Resonance Assignment of 19 KDa CD1 domain of Human Mitotic Check Point Serine/Threonine-Protein Kinase, Bub1

Jeong-Hwa Han

Seoul National University

 

P05  Solution Structure of AIP4 WW2 domain and its interaction with LMP2A PY motifs of Epstein-Barr Virus

Jin-Young Park

Seoul National University

 

P06  Physicochemical study of polyubiquitin-attached proteins

Erik Walinda

Department of Molecular Engineering, Kyoto University

 

P07  Crystal Structure of CofA, the Major Subunit of CFA/III Pili from Enterotoxigenic Escherichia coli

Shunsuke Fukakusa

Graduate School of Pharmaceutical Sciences, Osaka University

 

P08  Identification of Metabolic Differences between Hepatocellular Carcinoma and Hepatic Metastases from Colorectal Cancer using HR-MAS 1H NMR

Siwon Kim, Minji Lee, Dahye Yoon, and Suhkmann Kim

Busan National University

 

P09  NMR analysis of the carbohydrate binding domains of galectin-9, a modulator of the immune system

Yasuhiro Nonaka1, Takashi Ogawa1, Souichi Oomizu1, Shin-ichi Nakakita2, Nozomu Nishi2, Shigehiro Kamitori2, Mitsuomi Hirashima1, and Takanori Nakamura1

1Faculty of Medicine, Kagawa University, 2Life Science Research Center, Kagawa University

 

P10  Observations of amyloid aggregation at lipid bilayers and membrane disruption

Kenji Sasahara

Division of Structural Biology, Graduate School of Medicine, Kobe University

 

P11  Structure and biological function of anti-tumor complexes between oleic acid and proteins in the molten globule state

Takashi Nakamura1, Koki Makabe1, Tomoyasu Aizawa2, Keiichi Kawano2, Makoto Demura2 , and Kunihiro Kuwajima1

1Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, 2Division of Life Science, Graduate School of Life Science, Hokkaido University

 

P12  Structural and functional analyses of the ZFAT protein involved in auto-immune thyroid disease (AITD)

Naoya Tochio1, Takashi Umehara1, Kazuhiko Nakabayashi2, Takehiko Sasazuki3, Takanori Kigawa1,4, Senji Shirasawa5, Shigeyuki Yokoyama1,6

1RIKEN Systems and Structural Biology Center, 2Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development, 3Institute for Advanced Studies, Kyushu University, 4Department of Computational Intelligence and Systems Science, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, 5Department of Cell Biology, Faculty of Medicine, Fukuoka University, 6Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo

 

P13  Expression and Structural Analysis of a Recombinant Antibacterial Peptide, Cecropin P1

Taichi Nakazumi1, Chihiro Sakai1, Shiori Maehana1, Tomoyasu Aizawa1, Eri Hojo2, Takashi Matsumoto2, Masakatsu Kamiya1, Yasuhiro Kumaki1, Takashi Kikukawa1, Makoto Demura1, Keiichi Kawano1

1Faculty of Advanced Life Science, Hokkaido University, 2R&D Center, Nippon Meat Packers, Inc.

 

P14  Does Escherichia coli Thioredoxin Improve Expression Efficiency of Recombinant Peptide in Pichia pastoris?

Yuki Kitamura, Tomoyasu Aizawa, Masakastu Kamiya, Takashi Kikukawa, Makoto Demura, Keiichi Kawano

Graduate School of Life Science, Hokkaido University, Sapporo

 

P15  NMR Structural Analysis of Potato Antimicrobial Peptide Snakin-1

Rika Takahashi, Tomoyasu Aizawa, Masakatsu Kamiya, Takashi Kikukawa, Makoto Demura, Keiichi Kawano

Graduate School of Life Science, Hokkaido University,

 

P16  Expression, Purification, and NMR Characterization of the Cyclic Recombinant Form the First Extracellular Loop of the Transmembrane Protein Halorhodopsin

Yoshihiko Takanashi1, Masakatsu Kamiya1, Yasuhiro Kumaki2, Takashi Kikukawa1, Tomoyasu Aizawa1, Keiichi Kawano1, Makoto Demura1

1Graduate School of Life Science, Hokkaido University, Sapporo, 2Graduate School of Science, Hokkaido University

 

P17  Construction of a Novel Expression System for Cryptdin-4 by Using Inclusion Body Formation

Yuji Sato, Satoshi Tomisawa, Tomoyasu Aizawa, Naoki Sakai, Masakatsu Kamiya, Takashi Kikukawa, Yasuhiro Kumaki, Makoto Demura, Tokiyoshi Ayabe, Keiichi Kawano

Graduate School of Life Science, Hokkaido University

 

P18  NMR Structure and Interaction of Antimicrobial Peptide Tachyplesin I with Lipopolysaccharide

Takahiro Kushibiki1, Masakatsu Kamiya1, Tomoyasu Aizawa1, Yasuhiro Kumaki2, Takashi Kikukawa1, Makoto Demura1, Shunichiro Kawabata3, Keiichi Kawano1

1Graduate School of Life Science, Hokkaido University, 2Graduate School of Science, Hokkaido University, 3Department of Biology, Faculty of Sciences, Kyushu University

 

P19  Functional analysis of FMBP-1 molecular binding dynamics in vivo by Fluorescence Correlation Spectroscopy

Motosuke Tsutsumi1, Shohei Myoba1, Hideki Muto1, Mai Kimoto2, Tomoyasu Aizawa1,

Shigeharu Takiya2, Masataka Kinjo1, Makoto Demura1, Keiichi Kawano1

1Faculty of Advanced Life Science, Hokkaido University, 2Faculty of Science, Hokkaido University

 

P20  Volume change during the Cl- transport of Natronomonas pharaonis Halorhodopsin

Kousuke Shibasaki1, Takashi Kikukawa1 , Masakatsu Kamiya1, Tomoyasu Aizawa1, Keiichi Kawano1, Naoki Kamo2, Makoto Demura1

1Faculty of Advanced Life Science, Hokkaido University, 2College of Pharmaceutical Science, Matsuyama University

 

P21  Why does Anabaena sensory rhodopsin D217E mutant function as inwardly directed proton pump?

Takatoshi Hasemi1, Takashi Kikukawa1, Masakatsu Kamiya1, Tomoyasu Aizawa1, Keiichi Kawano1, Kwang-Hwan Jung2, Naoki Kamo3, Makoto Demura1

1Faculty of Advanced Life Science,Hokkaido University, 2Department of Life Science and Institute of Biological Interfaces, Sogang University, 3College of Pharmaceutical Sciences, Matsuyama University

 

P22  The study of novel thioredoxin derived from Pichia pastoris

Suguru Ichihashi1, Tsukasa Hamadate2, Yoshitaka Umetsu2, Hajime Tamaki1, Masakatsu Kamiya1, Takashi Kikukawa1, Yasuhiro Kumaki2, Tomoyasu Aizawa1, Makoto Demura1, Keiichi Kawano1

1Graduate school of life science Hokkaido University, 2Graduate school of science Hokkaido University

 

P23  Study of the oligomeric assembly of Gloeobacter rhodopsin in DDM solution

Takashi Tsukamoto1, Takashi Kikukawa1, Masakatsu Kamiya1, Tomoyasu Aizawa1, Keiichi Kawano1, Kwang-Hwan Jung2, Naoki Kamo3, Makoto Demura1

1Hokkaido Univ., 2Sogang Univ., 3Matsuyama Univ.

 

P24  Study of 7-TM membrane protein halorhodopsin using solid-state NMR

Hajime Tamaki1, Ayako Egawa2, Masakatsu Kamiya1, Takashi, Kikukawa1, Tomoyasu Aizawa1, Keiichi Kawano1, Toshimichi Fujiwara2, Makoto Demura1

1Graduate School of Life Science, Hokkaido University, 2Institute for Protein Research, Osaka University

 

P25  Solid State NMR Analysis of Gloeobacter Rhodopsin

Kouki Kido1, Hajime Tamaki2, Ayako Egawa3, Masakatsu Kamiya2, Takashi Kikukawa2, Tomoyasu Aizawa2, Keiichi Kawano2, Toshimichi Fujiwara3, Makoto Demura2

1School of Science, Hokkaido University, 2Graduate School of Life Science, Hokkaido University, 3Institute for Protein Research, Osaka University

 

P26  Structural Analysis of Bovine Osteocalcin in Acidic Condition

Takumi Ogasawara1, Keichi Kawano1, Takahisa Ikegami2

1Faculty of Advanced Life Science, Hokkaido University, 2Institute for Protein Research, Osaka University

 

P27  Aromatic-amide interactions in tripeptides Ar-Gly-Xaa as revealed by NMR

Yasuhiro Kumaki1, Manabu Nakano2, Masakatsu Kamiya2, Tomoyasu Aizawa2, Makoto Demura2, Keiichi Kawano2, Norio Matsushima3

1High-Resolution NMR Laboratory, 2Division of Biological Sciences, Graduate School of Science, Hokkaido University, 3School of Health Sciences, Sapporo Medical University

 

P28  Analysis of photo-induced proton transfer during the photocycle of Xanthorhodopsin

Takashi Kikukawa1, Yumi Sato1, Jun Tamogami1, Kazumi Shimono2, Masakatsu Kamiya1, Tomoyasu Aizawa1, Keiichi Kawano1, Makoto Demura1, Naoki Kamo2

1Grad. Sch. Life Sci., Hokkaido Univ., 2College Pharm. Sci., Matsuyama Univ.

 

P29  Structural and functional analysis of HCP

Masakatsu Kamiya1,2, Suguru Ichihashi1, Tomoyasu Aizawa1,2, Yasuhiro Kumaki3, Takashi Kikukawa1,2, Makoto Demura1,2, Yoichi Hayakawa4, and Keiichi Kawano1,2

1Graduate School of Life Science, Hokkaido University, 2Faculty of Advanced Life Science, Hokkaido University, 3Graduate School of Science, Hokkaido University, 4Department of Applied Biological Sciences, Saga University