創薬科学基盤イノベーションハブ:2010年度

  1. Mitsutake S., Kumada H., Soga M., Hurue Y., Asanuma F., Nagira M., Deguchi M., Date T., Yokose U., Inagaki Y., Sugiura M., Kohama T., Igarashi Y.
    Ceramide kinase is not essential but might act as an Ca2+-sensor for mast cell-activation
    Prostaglandins & Other Lipid Mediators, 93, 109-12(2010)

  2. Mitsutake S., Suzuki C., Akiyama M., Tsuji K., Yanagi T., Shimizu H., Igarashi Y.
    ABCA12 dysfunction causes a disorder in glucosylceramide accumulation during keratinocyte differentiation
    J. Dermatol. Sci, 60, 128-9( 2010)

  3. Ohno Y., Suto S., Yamanakaa M., Mizutani Y., Mitsutake S.,Igarashi Y., Sassa T., Kihara A.
    ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis
    Proc. Natl. Acad. Sci. 107, 18439-44( 2010)

  4. Fujiwara K., Kitatani K., Yazama H., Umehara H., Kikuchi M., Igarashi Y., Kitano H., Okazaki T.
    Inhibitory effects of dietary glucosylceramides on squamous cell carcinoma of the head and neck in NOD/SCID mice
    Int J Clin Oncol, Nov 6,( 2010)

  5. Okuda A.,1 Naganuma T., Ohno Y., Abe K., Yamagata M., Igarashi Y., Kihara A.
    Hetero-oligomeric interactions of an ELOVL4 mutant protein: implications in the molecular mechanism of Stargardt-3 macular dystrophy
    Molecular Vision, 16, 2438-45( 2010)

  6. Naruchi K., and Nishimura S-I., “Membrane-Bound Stable Glycosyltransferases: Highly Oriented Protein
    Immobilization by a C-Terminal Cationic Amphipathic Peptide”, Angew. Chem. Int. Ed., 80, 1328-1331( 2011)

  7. Hashimoto R., Fujitani N., Takegawa Y., Kurogochi M., Matsushita T., Naruchi K., Ohyabu N., Hinou H., Gao X-D., Manri N., Satake H., Kaneko A., Sakamoto T., and Nishimura S-I., “An efficient approach for the characterization of mucin type glycopeptides: The effect of O-glycosylation on the conformation of synthetic mucin peptides”, Chem. Eur. J., 17, 2393-2404( 2011)
  8. Irie T., Majima T., Sawaguchi N., Funakoshi T., Nishimura S.-I., and Minami A., “Biomechanical and histologic evaluation of tissue enjinnered ligaments using chitosan and hyaluronan hybrid polymer fibers: A rabbit medial collateral ligament reconstruction model”, J. Biomed. Mater. Res. Part A., 97A, 111-117( 2010)
  9. Miura Y., Kato K., Takegawa Y., Kurogochi M., Furukawa J.-i., Shinohara Y., Nagahori N., Amano M., Hinou H.,
    and Nishimura S-I., “Glycoblotting-assisted O-glycomics: Ammonium carbamate allows for highly efficient O-glycan release from glycoproteins”, Anal. Chem., 82, 10021-10029(2010)

  10.  Matsushita T,. Nagashima I., Fumoto M., Ohta T., Yamada K., Shimizu H., Hinou H., Naruchi K., Ito T., Kondo H., and Nishimura S-I., “Artificial Golgi apparatus: Globular protein-like dendrimer facilitates fully automated enzymatic glycan synthesis”, J. Am. Chem. Soc., 132: 16651-16656(2010)
  11. Ueda T., Ito T., Tomita K., Togame H., Fumoto M., Asakura K., Oshima T., Nishimura S-I., and Hanasaki K., “Identification of glycosylated exendin-4 analogue with prolonged blood glucose-lowering activity through glycosylation scanning substitution”, Bioorg. Med. Chem. Lett., 20, 4631-4634( 2010)
  12. Shimizu H. K., Hosoguchi K., Liu Y., Fujitani N., Ohta T., Hinou H., Matsushita T., Shimizu H., Feizi T., and Nishimura S.-I.“, Chemical synthesis, folding and structural insights into O-fucosylated epidermal growth factor-like repeat 12 of mouse Notch-1 receptor”, J. Am. Chem. Soc., 132, 14857-14865( 2010)
  13. Hosoguchi K., Maeda T., Furukawa J.-i., Shinohara Y., Hinou H., Sekiguchi M., Togame H., Takemoto H., Kondo H., and Nishimura S.-I., “An Efficient Approach to the Discovery of Potent Inhibitors against Glycosyltransferases”, J. Med. Chem., 53, 5607-5619( 2010)
  14. Hashimoto R., Hirose K., Sato T., Fukushima N., Miura N., and Nishimura S.-I.,“ Functional network of glycan-related molecules: Glyco-Net in Glycoconjugate Data Bank”, BMC. Syst. Biol., 4:91( 2010)
  15. Kurogochi M., Matsushita T., Amano M., Furukawa J.-i., Shinohara Y., Aoshima M., and Nishimura S.-I., “Sialic acid-focused quantitative mouse serum glycoproteomics by multiple reaction monitoring assay”, Mol. Cell. Proteomics., 9, 2354-2368( 2010)
  16. Yoshimura Y., Matsushita T., Fujitani N., Takegawa Y., Fujihira H., Naruchi K., Gao, X-D., Manri N., Sakamoto T., Kato K., Hinou H., and Nishimura S.-I., “Unexpected Tolerance of Glycosylation by UDP-GalNAc:Polypeptide alpha-N-Acetylgalactosaminyltransferase Revealed by Electron Capture Dissociation Mass Spectrometry: Carbohydrate as Potential Protective Groups”, Biochemistry, 49, 5929-5941( 2010)
  17. Carvalho S., Sola-Penna M., Oliveira I., Pita S., Goncalves A., Neves B., Sousa F., Freire-de-Lima., Kurogochi M., Hinou H., Nishimura S.-I., Previato J., and Todeschini A., “A new class of mechanism-based inhibitors for Trypanosama cruzi trans-sialidase and their influence on parasite virulence”, Glycobiology, 20, 1034-1045( 2010)
  18.  Izumikawa T, Kanagawa N, Watamoto Y, Okada M, Saeki M, Sakano M, Sugahara K., Sugihara K, Asano M, Kitagawa H. Impairment of embryonic cell division and glycosaminoglycan biosynthesis in glucuronyltransferase-I-deficient mice. J. Biol. Chem., 285, 12190-12196( 2010)
  19. Akatsu C, Fongmoon D, Mizumoto S, Jacquinet J-C, Kongtawelert P, Yamada S, Sugahara K.
    Development of a mouse monoclonal antibody against the chondroitin sulfate-protein linkage region derived from shark cartilage.
    Glycoconjugate J., 27, 387-399( 2010).

  20. *Miyake N, *Kosho T, *Mizumoto S, Furuichi T, Hatamochi A, Nagashima Y, Arai E, Takahashi K, Kawamura R, Wakui K, Takahashi J, Kato H, Yasui H, Ishida T, Ohashi H, Nishimura G, Shiina M, Saitsu H, Tsurusaki Y, Doi H, Fukushima Y, Ikegawa S, Yamada S, Sugahara K, Matsumoto N(. *Equal contribution)
    Loss-of-function mutations of CHST14 in a new type of Ehlers-Danlos syndrome.
    Hum. Mutat., 31, 966-974( 2010).

  21. Adamczyk P, Zenkert C, Balasubramanian PG, Yamada S, Murakoshi S, Sugahara K, Hwang JS, Gojobori T, Holstein TW, Özbek S.
    A non-sulfated chondroitin stabilizes membrane tabulation in cnidarian organelles.
    J. Biol. Chem., 285, 25613-25623( 2010).

  22. Basappa, Murugan S, Kavitha CV, Purushothaman A, Nevin KG, Sugahara K, Rangappa KS.
    A small oxazine compound as an anti-tumor agent: A novel pyranoside mimetic that binds to VEGF, HB-EGF, and TNF-α.
    Cancer Lett., 297, 231-243( 2010).

  23. Tamura J, Nakamura-Yamamoto T, Nishimura Y, Mizumoto S, Takahashi J, Sugahara K.
    Synthesis of the glycosaminoglycan-protein linkage tetraosyl peptide moieties of betaglycan, which serve as a hexosamine acceptor for enzymatic glycosyl transfer.
    Carbohydr. Res., 345, 2115-2123( 2010).

  24. Li F, Nandini CD, Hattori T, Bao X, Murayama D, Nakamura T, Fukushima N, Sugahara K.
    Structure of pleiotrophin- and hepatocyte growth factorbinding sulfated hexasaccharide determined by biochemical and computational approaches.
    J. Biol. Chem., 285, 27673-27685( 2010).

  25. Nakahashi A., Yaguchi Y., Miura N., Emura M., Monde K.
    A Vibrational Circular Dichroism Approach to the Determination of the Absolute Configurations of Flavorous 5-Substituted-2( 5H) -furanones
    J. Nat. Prod., 74, 707-711( 2011).

  26. Aoyagi Y., Yamazaki A., Kato R., Tobe F., Fukaya H., Nishikawa T., Nakahashi A., Miura N., Monde K., Takeya K.
    Salvileucalin C, a novel rearranged neoclerodane diterpene from Salvia leucantha
    Tetrahedron Lett., 52, 1851-1853( 2011).

  27. Kuwahara S., Obata K., Fujita T., Miura N., Nakahashi A., Monde K., Harada N.
    (R)-(+)-[VCD(–)984]-4-Ethyl-4-methyloctane: A Cryptochiral Hydrocarbon with a Quaternary Chiral Center.
    (2) Vibrational CD Spectra of Both Enantiomers and Absolute Configurational Assignment
    Eur. J. Org. Chem., 33, 6385–6392( 2010).

  28. Fujita T., Obata K., Kuwahara S., Nakahashi A., Monde K., Decatur J., Harada N.
    (R)-(+)-[VCD(–)984]-4-Ethyl-4-methyloctane: A Cryptochiral Hydrocarbon with a Quaternary Chiral Center.
    (1) Synthesis of the Enantiopure Compound and Unambiguous Determination of Absolute Configuration
    Eur. J. Org. Chem., 33, 6372–6384( 2010)

  29. Hinou H., Miyoshi R., Takasu Y., Kai H, Kurogochi M., Arioka S., Gao X.-D., Miura N., Fujitani N., Shinya Otomo, Yoshinaga T., Fujiwara T., Noshi T., Togame H., Takemoto H., Nishimura S.-I.
    Novel Strategy for Neuraminidase Inhibitors Using Mechanism-based Labeling Information
    Chem. Asian J., 2011, 1048-1056( 2011)

  30. Feng F.†, Miura N.†, Isoda N., Sakoda Y., Okamastu M., Kida H., Nishimura S.-I.
    Novel trivalent anti-influenza reagent
    Bioorganic & Medicinal Chemistry, 20, 3772-3776( 2010)
    †These authors are equally contributed.

  31. Arioka S., Sakagami S., Uematsu R., Yamaguchi H., Togame H., Takemoto H., Hinou H., Nishimura S.-I.
    Potent inhibitor scaffold against Trypanosoma cruzi transsialidase
    Bioorg. Med. Chem., 18, 1633-1640( 2010).

  32. Hashimoto R., Fujitani N., Takegawa T., Kurogochi M., Matsushita T., Naruchi K., Ohyabu N., Hinou H., Gao X.D., Manri N., Satake H., Kaneko A., Sakamoto T., Nishimura S.-I.
    An efficient approach for the characterization of mucin type glycopeptides: The effect of O-glycosylation on the conformation of synthetic mucin peptides
    Chem. Eur. J., 17, 2393-2404( 2011).

  33. Yuyama K., Yanagisawa K.
    Sphingomyelin accumulation provides a favorable milieu for GM1 ganglioside-induced assembly of amyloid betaprotein.
    Neurosci. Lett., 481, 168-172( 2010).

  34. Yuyama K., Sekino-Suzuki N., Yamamoto N., Kasahara K.
    Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.
    J. Neurochem. 116, 845-850( 2011).

  1. 西村紳一郎
    糖鎖研究と創薬:バイオマーカーとしての糖鎖とその可能性
    中国・日本科学最前線-研究の現場から-2011年版,独立行政法人 科学技術振興機構(JST)中国総合研究センター, 335-338( 2011)

  2. 西村紳一郎
    全自動糖鎖解析システムの開発-臨床検査バイオマーカーとしての糖鎖の可能性-
    医療と検査機器・試薬, ラボサービス/宇宙堂八木書店, 34( 1), 53-60( 2011)

  3.  Iwasaki N., Kasahara Y., Yamane S., Igarashi T., Minami A., and Nishimura S-I.
    Chitosan-Based Hyaluronic Acid Hybrid Polymer Fibers as a Scaffold Biomaterial for Cartilage Tissue Engineering
    Polymers, 3, 100-113( 2011)

  4. 天野麻穂、三浦嘉晃、西村紳一郎
    グライコブロッティング法が明らかにする翻訳後修飾の意義と大規模糖鎖解析への展開
    生化学、日本生化学会, 第83巻 第1号, 5-12( 2011)

  5. 古川潤一、西村紳一郎
    グライコブロッティング法と糖鎖自動抽出装置(SweetBlotⓇ)による一滴の血清から糖鎖バイオマーカー探索
    未来材料、株式会社エヌ・ティー・エス, 10, 48-54(2010)

  6. Amano M., Nishimura S-I.
    Large-Scale Glycomics for Discovering Cancer-Associated N-Glycans by Integrating Glycoblotting and Mass Spectrometry
    Methods in Enzymology, 478, 109-125( 2010)

  7. Matsushita T., Nishimura S-I.
    Novel Synthesis of Functional Mucin Glycopeptides Containing Both N- and O-Glycans
    Methods in Enzymology, 478, 485-502(2010)

  8. Mizumoto S.
    Roles of Dermatan Sulfate Epimerase.
    Trend. Glycosci. Glycotechnol., 22, 256-258(2010).

  9. Taniguchi T., Monde K.
    Electronic Circular Dichroism and Vibrational Circular Dichroism of Glycoconjugates,
    Ed. Nina Berova, Prasad Polavarapu, Koji Nakanishi, Robert Woody,
    Comprehensive Chiroptical Spectroscopy,
    John Wiley & Sons, in press.( 2011)

  10. Hinou H., Kai H., Nishimura S.-I.
    Mechanism-based Probing, Characterization, and Inhibitor Design of Glycosidases and Glycosyltransferases
    Methods in Molecular Biology, in press.

  (招待講演)

  1. June 2010
    Sapporo, Japan
    27th Naito Conference
    Through lipid signaling to lipid dynamics: a new challenge in sphingolipid biology.
    Mitsutake S., Mizutani Y. Yuyama K. & Igarashi Y.

  2. September 2010
    Bilbao, Spain
    51st ICBL Conference
    Ceramide metabolism and membrane dynamics
    Igarashi Y

  3. June 2010
    Tsukuba, JAPAN
    第58回質量分析総合討論会、第1回アジア・オセアニア
    質量分析会議
    Glycoblotting and mass spectrometry for the discovery of new biomaekers
    Nishimura S-I.

  4. June 2010
    Singapore
    BIT’s 3rd World Cancer Congress 2010
    Large-scale glycomics for the discovery of cancer markers: Diagnostic mass spectrometry based on glycoblotting technology
    Nishimura S-I.

  5. August 2010
    Boston, USA
    The 240th ACS National Meeting
    Cancer relevant epitopes uncovered by synthetic mucin glycopeptides
    Nishimura S-I.

  6. September 2010
    Sapporo, JAPAN
    JAACT2010 (Animal Cell Technology from Asis to World)
    Glycoblotting method reveals new promising biomarkers during cell differentiation.
    Nishimura S-I.

  7. Hawaii, USA
    2010 International Chemical Congress of Pacific Basin Societies( PACIFICHEM2010)
    Glycoblotting-assisted high throughput glycomics and glycoproteomics
    Nishimura S-I.

  8. February 2011
    Mysore, INDIA
    International Symposium on Challenges in Drug Discovery Programme 2011( ISCDDP-2011)
    Fully automated glycan analyzer: Importance of Indian glycan database both for early stage diagnosis of Indian and for discovery research of Indian-responsible therapeutic reagents
    Nishimura S-I.

  9. January, 2011
    Trivandrum, Kerala, INDIA
    9th International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules
    Chondroitin sulfate-specific novel hydrolase in human.
    Yamada S.

  10. January, 2011
    Trivandrum, Kerala, INDIA
    9th International Symposium on Biochemical Roles of Eukaryotic Cell Surface Macromolecules
    Chondroitin sulfate E type structure at tumor cell surface is involved in the experimental metastasis of mouse tumor cell lines.
    Sugahara K.

  11. Sep. 2nd, 2010.
    Barcelona, Spain
    Barcelona Biomed Seminer-Chemistry and Molecular Pharmacology Programme
    Novel Strategies for Neuraminidase Inhibitors using Suicide Substrates
    Hinou H.

  12. Sep. 17th, 2010.
    Barcelona, Spain
    Seminario Excepcional de Grupo-Chemistry and MolecularPharmacology Programme
    Peptide Cyclization in Fluorinated Alcohol: Toward Cyclic AFGP
    Hinou H.

    (口頭発表)

  13. August, 2010
    Chiba, JAPAN
    The 25th International Carbohydrate Symposium( ICS2010)
    Identification of a chondroitin sulfate-specific novel hydrolase in human.
    Kaneiwa T, Mizumoto S, Sugahara K, Yamada S.

  14. March, 2011
    Sapporo, JAPAN
    International Fusion-Bioscience Symposium for young researchers
    Identification of chondroitin sulfate-specific novel hydrolases.
    Kaneiwa T, Mizumoto S, Sugahara K, Yamada S.

  15. July 2010
    Sapporo, JAPAN
    21st International Symposium on Chirality, Chirality 2010
    Vibrational Circular Dichroism & Its Application in Bimolecular Science
    Monde K., Nakahashi A., Shibata M., Taniguchi T., Saito S., Hammam M. A. S., Miura N.

  16. August, 2010
    Tokyo (Chiba), JAPAN
    The 25th International Carbohydrate Symposium(ICS2010)
    Vibrational Circular Dichroism (VCD) Studies of Carbohydrates and Glycoconjugates
    Monde K., Nakahashi A., Taniguchi T., Shibata M., Fukuzawa M., Miura N.

  17. March 2011
    New York, U.S.A.
    Hunter Collage Special Organic Lectures
    Vibrational Circular Dichroism & Its Application in Bimolecular Science
    Monde K.

  18. Mar. 21th-25th, 2010.
    San Francisco (CA), USA
    American Chemical Society Spring 2010 National Meeting & Exposition
    Novel Strategy for Neuraminidase Inhibitors Using Mechanism-based probe
    Hinou H., Miyoshi R., Takasu Y., Kai H., Kurogochi M., Gao X.-D., Nishimura S.-I.

  1. 2010年4月3日
    札幌市
    JSTプラザ成果発表会
    スキンケアチップを利用したスフィンゴ脂質 セラミド等の皮膚機能改善への応用
    五十嵐靖之

  2. 2010年4月12日
    札幌市
    北海道科学技術会議
    スキンケアチップを利用したスフィンゴ脂質 セラミド等の皮膚機能改善への応用
    五十嵐靖之

  3. 2010年5月28日
    赤穂市
    アース製薬研究所
    生命の音楽、遺伝子絶対主義に対する反論
    五十嵐靖之

  4. 2010年6月
    札幌市
    The 27th Naito Conference on Membrane Dynamics and Lipid Biology
    SMS2 deficiency prevents diet-induced obesity.
    光武 進、横田はづき、座間宏太、山下 匡、岡崎俊朗、渡辺 研、五十嵐靖之

  5. 2010年6月
    札幌市
    The 27th Naito Conference on Membrane Dynamics and Lipid Biology
    Ceramide biosynthesis in keratinocyte and its regulation by PPAR activators.
    Mizutani Y., Kihara A. & Igarashi Y.

  6.  2010年6月
    札幌市
    The 27th Naito Conference on Membrane Dynamics and Lipid Biology
    Spfingolipids as a possible signature of microbial communities in actyivated sluge and potential of fungi to wastewater treatment under cold conditions.
    Kushi Y., Tsuge J., Kanemura Y., Yanagida M., Shiotani K., Okamoto M. & Igarashi Y.

  7. 2010年7月26日
    東京都
    SSKセミナー「セラミドとキノコ」
    セラミドの生理機能から食の科学へー 特に皮膚組織におけるセラミド合成とその機能
    五十嵐靖之

  8. 2010年8月
    札幌市
    札幌エスティック研究会講演会
    セラミド合成と皮膚機能
    五十嵐靖之

  9. 2010年11月
    東京
    平成22年度 秋季有機合成化学講習会
    疾患マーカー探索研究を加速する有機合成化学
    西村紳一郎

  10. 2010年11月
    札幌市
    第42回 未来創薬・医療イノベーションセミナー
    プロテオグリカンの多糖側鎖の生物活性と創薬の可能性
    菅原一幸

  11. 2011年1月20日
    桐生市
    群馬大学工学部特別講演会
    O-結合型糖ペプチド合成法の開発と機能解析
    比能 洋

  1.  2010/6/21(特願2010-140991)
    「糖鎖修飾剤、糖鎖を製造する方法および薬剤の品質を評価する方法」
    発明者:西村紳一郎、天野麻穂、黒河内政樹、松下隆彦

  2. 2010/9/14(PCT/JP2010/065842)
    「腎細胞癌の判定法」
    発明者:西村紳一郎、三浦嘉晃、中原拓、天野麻穂、大山 力、畠山真吾

  3.  2010/9/29(PCT/JP2010/005856)
    「糖鎖分析による婦人科がんの診断方法」
    発明者:西村紳一郎、三浦嘉晃、中原 拓、天野麻穂、青木大輔、鈴木 淳

  4. 2010/5/6(特許第4451036号)
    新規コンドロイチン合成酵素
    発明者:菅原一幸、北川裕之

  5. 2010/5/21(特許第4514708号)
    コンドロイチン重合化因子の同定と、その利用
    発明者:菅原一幸、北川裕之

  6. 2010/10/29(特願2010-244708号)
    硫酸化多糖または硫酸化オリゴ糖の分析方法、硫酸化多糖または硫酸化オリゴ糖を含む薬学的組成物および医薬、薬学的組成物および医薬の製造方法、疾患の治療、診断、症状の軽減および予防方法
    発明者:菅原一幸

  7. 2010/12/13(特願2006-084323 手続き補正書提出)
    糖鎖マーカー認識プローブ、それを用いた神経突起伸長阻害剤、ニューロン染色剤、腫瘍細胞染色剤、免疫組織染色剤、薬学的組成物および医薬
    発明者:菅原一幸

  8. 2011/1/14(特許第4662385号)
    硫酸化多糖または硫酸化オリゴ糖の分析方法、硫酸化多糖または硫酸化オリゴ糖を含む薬学的組成物および医薬、薬学的組成物および医薬の製造方法
    発明者:菅原一幸

  1. Very Important Paper (VIP): Hiroshi Hinou*, Risho Miyoshi, Yasuaki Takasu, Hirokazu Kai, Masaki Kurogochi, Shingo Arioka, Xiao-Dong Gao, Nobuaki Miura, Naoki Fujitani, Shinya Otomo, Tomokazu Yoshinaga, Tamio Fujiwara, Takeshi Noshi, Hiroko Togame, Hiroshi Takemoto, Shin-Ichiro Nishimura* “Novel Strategy for Neuraminidase Inhibitors Using Mechanism-based Labeling Information” Chem. Asian J(. 2011).
  2. Best Poster Award: “Synthesis and Evaluation of Novel Cyclic Sialidase Suicide Substrate” International Symposium on Challenges in Drug Discovery Programme, February 16-17th, 2011, Mysore, India.